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作 者 ：张英 姚拓 朱颖

期 刊 ：植物营养与肥料学报 2012年 18卷 5期 页码：1286-1294

关键词：复合接种剂；三叶草；生长特性；品质；

Keywords:Compound inoculant, Trifolium pretense L., growth characteristic, quality,

摘 要 ：利用从兰州地区红三叶 (Trifolium pratense L.) 和白三叶（Trifolium repens L.）根际分离、 筛选获得的4株植物根际促生细菌（代号：A-Hsg、 B-ls13、 C-lhs11、 D-lhs14) 制作微生物接种剂，采用盆栽法测定不同接种剂处理对红三叶草生长特性及品质的影响。结果表明，本研究制作的接种剂符合《农用微生物菌剂》质量标准（GB 20287-2006）。复合接种剂处理AD对红三叶草株高、 根长促进作用显著 (P<0.05)，分别较对照增加33.62%和46.20%。AB和AD处理红三叶草地上植物量增重显著，较对照增加了101.69%和94.98%。BC和AD处理对地下植物量增加显著，增加了197.63%和151.18%。AC、 AD和BCD处理后红三叶草整株磷含量均高于对照，其中BCD处理较对照差异显著（P<0.01），磷含量较对照增加了15.38%。ABCD处理红三叶草整株的含氮量较对照达到了显著水平（P<0.05），较对照增加了14.77%。ABD、 BCD和ABCD处理均可提高红三叶草地上部分粗蛋白含量，其中处理ABCD粗蛋白含量较对照增加最多，增加了14.62%，达到了显著水平（P<0.05）。复合接种剂AD、 AB、 BC、 AC、 ABD、 BCD和ABCD接种后，对红三叶草生长特性和品质的提高表现出良好的促进效果，可作为复合菌肥材料作进一步研究。

Abstract:Four PGPR(Strain No.: A-Hsg， B-ls13， C-lhs11， D-lhs14) strains isolated and screened from the red clover (Trifolium pratense) and the white clover (Trifolium repens) in rhizosphere in Lanzhou were used to produce single or compound inoculum. A pot experiment was carried out to determine effects of the inoculum on growth character and quality of red clover. The results indicate that the produced inoculants are accorded with the quality criterion of agricultural microbial fertilizer ofChina(GB 20287-2006). The compound inoculant treatment, AD, has a significant effect on plant height and root length which are enhanced 3362% and 4620% respectively compared to the control (P<0.05). The compound inoculant treatments, AB and AD, could improve the aboveground biomass by 101.69% and 94.98% respectively comparing to the control (P<0.05), while the compound inoculant treatment, BC and AD, could increase underground biomass by 197.63% and 151.18% (P<0.05) respectively. The phosphorous contents of the plants treated by the compound inoculants (AC, AD and BCD) are increased compared to control. Moreover, the compound inoculants (ABCD) enhances nitrogen content significantly (14.77%) compared to the control (P<0.05). Three treatments, ABD, BCD and ABCD, could improve the crude protein contents, and the crude protein content of the treatment of ABCD is improved by 14.26% compared to the control (P<0.05). Thus, the compound inoculants (AD, AB, BC, AC, ABD, BCD and ABCD) show good promoting effects on the growth and the quality of the red clover, and can be used as compound biofertilizer for the further study.

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