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作 者 ：余朝阁 黄欣阳 李天来 刘志恒

期 刊 ：植物营养与肥料学报 2013年 19卷 6期 页码：1445-1449

关键词：番茄；钙；化学诱抗剂；木质素；

Keywords:tomato, Calcium, chemical elicitor, lignin,

摘 要 ：为探索钙对化学诱抗剂水杨酸（SA）、 茉莉酸甲酯（MeJA）、 龙胆酸（GA）和 β-氨基丁酸（BABA）诱导番茄木质素合成的影响，在番茄六叶期用上述诱抗剂分别处理第3 叶片，然后喷施CaCl₂、 蒸馏水、 乙二醇-双-(2-氨基乙醚)四乙酸（EGTA）或LaCl₃，处理后0、 1、 2、 3、 5 d 测定各处理番茄第3 叶片（诱导叶）和第5 叶片（非诱导叶）中木质素含量的变化。结果表明, （1）用上述化学诱抗剂处理番茄第3 叶片，诱导叶及其上位非诱导叶中木质素含量均升高；（2）外源Ca²⁺ 进一步促进上述诱抗剂诱导番茄叶片中木质素含量的增加；而Ca²⁺ 螯合剂EGTA和质膜钙通道抑制剂LaCl₃，则不同程度地抑制这些诱抗剂对木质素含量的诱导作用。这些结果表明，钙对上述4 种诱抗剂诱导番茄叶片中木质素的合成具有正调控作用。

Abstract:The effects of calcium on synthesis of lignin induced by chemical elicitors in tomato were investigated in this study. The 3rd leaves were smeared with chemical elicitors:   salicylic acid(SA),  Methyl jasmonate(MeJA),  β-aminobutyric acid(BABA) and gentisic acid(GA) separately at the sixleaf stage of tomato. After the third leaves dried under room temperature,  all the tomato plants were sprayed with CaCl₂,  distilled water,  EGTA and LaCl₃ in turn.  The contents of lignin in the third leaves(induced) and the fifth leaves(un-induced) were determined in 0,  1,  2,  3 and 5 days after treatments. The results show that:  (1) The lignin content in both the induced leaves and un-induced leaves increased after treated with chemical elicitors. (2) The lignin synthesis in treated tomato leaves were further promoted by exterior Ca²⁺,  but inhibited by Ca²⁺ chelator EGTA and the plasmalemma Ca²⁺ channel blocker La^{3 +} in different levels. These results indicated that Ca²⁺ play a positive regulation role in synthesis of lignin in tomato leaves induced by the above four chemical elicitors.

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