Abstract:This study aimed to evaluate the effects of longterm addition of organic and inorganic amendments on relationship between soil microbial and biochemical properties varying microbial indicators based on Fu’an experimental field site in Fujian, China. Under half rate of chemical fertilizer + half rate of organic fertilizer, whole rate of organic fertilizer, whole rate of chemical fertilizer + leguminous green manure and half rate of chemical fertilizer + half rate of organic fertilizer + leguminous green manure treatments, soil organic contents, total numbers of culturable microbes, microbial biomass C, microbial biomass N and soil enzyme activities were significant enhancement, except inorganic fertilize treatment compared with control treatment. As compared to other treatments, “inorganic fertilizer + organic manure + leguminous green manure” treatment observed the highest number of culturable microbes, microbial biomass and enzyme activities, whereas treatment with inorganic fertilizer decreased the number of soil organisms, enzyme activities and the stability of tea orchard soil ecosystem. Furthermore, microbial community was determined by signature phopholipid fatty acids (PLFAs), showed obvious change under various fertilization strategies. The correlation between microbial biomass with phenotypic groups of organisms and cultural microbes was higher than those of culturable microbes and enzyme activities, indicating that the size of the microbial community showed intensify impact on its composition rather than on enzyme functional capacity in tea orchard soil ecosystem. It is suggested that soil microbial indicators could reflect different soil fertility levels. Thus, taking various measurements were considered as a good technique for evaluating tea orchard soil quality more objectively at eastern Fujian district.