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作 者 ：樊怀福1, 2, 杜长霞1, 郭世荣1*, 李 娟1

期 刊 ：植物营养与肥料学报 2010年 16卷 5期 页码：1224-1231

关键词：一氧化氮；NaCl胁迫；黄瓜幼苗；活性氧；氯化镧；

Keywords:nitric oxide, NaCl stress, cucumber seedling, active oxygen, LaCl3,

摘 要 ：采用营养液培养的方法，研究了Ca ²⁺对外源一氧化氮（Nitric oxide, NO）所诱导的NaCl胁迫下黄瓜幼苗生长、活性氧代谢的影响。结果表明，添加外源NO或Ca ²⁺显著缓解了NaCl胁迫对黄瓜幼苗生长的抑制，叶片和根系超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性较单独NaCl胁迫处理显著提高，丙二醛（MDA）和过氧化氢（H₂O₂）的含量、超氧阴离子（O₂^-•）产生速率明显下降; 添加NO的同时添加Ca²⁺通道抑制剂La ³⁺抑制了NO的这些调节作用。结果表明Ca ²⁺对NO诱导的NaCl胁迫下黄瓜幼苗植株活性氧清除能力的提高起重要作用，NO的作用可能依赖于胞浆Ca ²⁺ 。

Abstract:The study was conducted in a nutrient solution to investigate the effects of Ca ²⁺ on growth and active oxygen metabolism of cucumber seedlings induced by exogenous nitric oxide under the 50 mmol/L NaCl stress. The results show that plasma membrane Ca ²⁺ channel blockers La³⁺ block the effects on growth and active oxygen metabolism induced by exogenous nitric oxide. Exogenous nitric oxide or Ca2+ alleviate the injury to seedlings under the 50 mmol/L NaCl stress significantly, and the seedling growth, activities of SOD, POD and CAT are increased and the rate of O₂^-• production, contents of MDA and H₂O₂ are decreased. These results indicate the La³⁺ blocks those effects induced by exogenous nitric oxide under the NaCl stress, and Ca ²⁺ might play an important role in growth and active oxygen metabolism induced by exogenous nitric oxide. The effect of nitric oxide here depends on the content of Ca ²⁺ in cytoplasm.

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