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Influence of Simulated Warming to the Carbon, Nitrogen and Their Stability Isotope (δ13C,δ15N) Contents in Alpine Meadow Plant Leaves

模拟增温对高寒草甸植物叶片碳氮及其同位素δ13C和δ15N含量的影响



全 文 :书!"#$%&
,2016,36(4):0777-0783
犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
  !"#$:10004025(2016)04077707               犱狅犻:10.7606/j.issn.10004025.2016.04.0777
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:20151230;)*&%+(:20160407
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(OTC1)‘(OTC2)’“st‘=,”•–—˜™š›œ—•žš
Ÿ ¡
(δ13C、δ15N)¢£¤Gst3¥¦zXY。§¨©ª:«~TG¬‘st‘=(OTC2)d C/N­®­w¯
°±–14.1%,—²$C/NG’³st´µ¶·¸gs¹,º»¼½¾¿À(犘>0.05)。4#$˜™δ13C®
G-24.12‰~-28.34‰ÁÂ,~T˜™δ13C®ÃstÄÅR,†‡、ˆ‰ŠÃstÄ°±,Æ~TGOTC2
zδ13C®XYÇÈ¿ÀÉÊ(犘<0.05)。~Tˆ‰Šz˜™δ15N®GOTC1OTC2d½­w¯s¹,ƈ
‰Šs¹¬¿À
(犘<0.05)。‚ƒ„…TGOTC1OTC2z˜™δ15N®­w¯ËÌÍÎ18.7%26.9%,»
¼·¾¿À
(犘>0.05);†‡˜™δ15N®GOTC2Ï­w¯±11.0%(犘>0.05),GOTC1zÏ­w¯R2.8%(犘
>0.05)。ÐÑ,RS~TT€¾ŸÒÓÔ#$$š›Õ֜•žš›Ÿ ¡ÕÖw×Øst¸¾Ÿzy
vqِÚÛ

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qrst

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;C/N­;δ13C;δ15N
STUV$
:Q948.112+.2 !WXYZ:A
犐狀犳犾狌犲狀犮犲狅犳犛犻犿狌犾犪狋犲犱犠犪狉犿犻狀犵狋狅狋犺犲犆犪狉犫狅狀,犖犻狋狉狅犵犲狀犪狀犱犜犺犲犻狉
犛狋犪犫犻犾犻狋狔犐狊狅狋狅狆犲(δ13犆,δ15犖)犆狅狀狋犲狀狋狊犻狀犃犾狆犻狀犲犕犲犪犱狅狑犘犾犪狀狋犔犲犪狏犲狊
ZHAOYanyan1,3,XULonghua1,3,YAOBuqing1,2,MAZhen1,2,ZHANGChunhui1,2,
WANGFangping1,3,ZHOUHuakun1,2
(1.NorthwestPlateauInstituteofBiology,ChineseAcademyofSciences,Xining810008,China;2.KeyLaboratoryofRestora
tionEcologyforColdRegionsinQinghai,Xining810008,China;3.UniversityofChineseAcademyofSciences,Beijing100049
China).
犃犫狊狋狉犪犮狋:Basedontheresearchmethodsthatsimulatedwarmingeffectonplantsoftundraplan(ITEX),
weusedfourkindsofplantsatalpine犓狅犫狉犲狊犻犪犺狌犿犻犾犻狊meadow:犓.犺狌犿犻犾犻狊,犈犾狔犿狌狊狀狌狋犪狀狊,犗狓狔狋狉狅狆犻狊
狅犮犺狉狅犮犲狆犺犪犾犪,犌犲狀狋犻犪狀犪狊狋狉犪犿犻狀犲犪,setabig(OTC1)andasmal(OTC2)warmingroom,todeterminethe
carbon,nitrogenandtheirstabilityisotopes(δ13C,δ15N)contentchangesafterthreeyearsofwarming.The
resultshowedthat:inadditiontotheC/Nratioof犓.犺狌犿犻犾犻狊wasreducedby14.1%insmalwarming
room(OTC2),theC/Nratiosoftheotherthreespecieswerealincreasedinthetwowarmingroomsthan
thatinthecontrol,whilethedifferenceswerealnotsignificant(犘>0.05).Theδ13Cofthefourkindsof
plantleaveswasbetween-24.12‰ ~ -28.34‰,theδ13Cof犓.犺狌犿犻犾犻狊leafwasincreasedwithraising
temperature,whiletheδ13Cof犗.狅犮犺狉狅犮犲狆犺犪犾犪and犌.狊狋狉犪犿犻狀犲犪wasreducedwiththeraisingtempera
ture.Theδ13Cchangeof犓.犺狌犿犻犾犻狊leafreachedsignificantlevel(犘<0.05).Theδ15Nof犓.犺狌犿犻犾犻狊and
犌.狊狋狉犪犿犻狀犲犪werealincreasedinthetwowarmingroomsthanthatinthecontrol,theδ15Nchangeof犌.
狊狋狉犪犿犻狀犲犪reachedsignificantlevel(犘<0.05);theδ15Nchangeof犈.狀狌狋犪狀狊reduced18.7%and26.9%in
OTC1andOTC2respectively,whilethedifferenceswerealnotsignificant(犘>0.05);theδ15Nchangeof
犗.狅犮犺狉狅犮犲狆犺犪犾犪wasreducedby11.0%insmal warmingroom (OTC2)thanthatincontrol,whilein
creasedby2.8%inbigwarmingroom(OTC1)thanthatincontrol.Itisobservedthatthecarbon,nitro
genandtheirstabilityisotopes(δ13C,δ15N)ofdifferentfunctionalgroupofplantspecieshavedifferentre
sponsepatternsandrulestoshorttermwarming.
犓犲狔狑狅狉犱狊:simulatedwarming;犓狅犫狉犲狊犻犪犺狌犿犻犾犻狊meadow;representativeplants;C/Nratio;δ13C;δ15N
  šÝ9OzMÞß¡,)*àdš}12C、13C、
14C¢áŸ ¡zâÙãG,—d12C、13Cäwå
æË̋98.89%1.11%,ç*$èzšŸ ¡
éêë
13C/12C­®ì•zδ(13C)©í。#$îï
]ðÝ)*àšŸ ¡Ëñz9Oòó

Gîïò
ód

#$˜™ôõnŸYöáz
12CO2 Ä÷ø
13CO2,ùúû#$éüd13Cz)*åæý±nl
VCO2,GVþÿË!"#ù$%&©%ûö¹ª
¿
[1]。
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13CzËñKLê‹#$MZ%(
)š*+KLz,-.7Á$

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(ITEX)qrstuvw#$xyzKL{|[10],
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ðâãäå

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=

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1.15m,Ûïì[ží‹1.00、0.70m,ÙÚRæ½
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T€]w¯
(CK)。
`KL˜™~TT€dz~T
(犓狅犫狉犲狊犻犪
犺狌犿犻犾犻狊)、†‡(犗狓狔狋狉狅狆犻狊狅犮犺狉狅犮犲狆犺犪犾犪)、‚ƒ„
…T
(犈犾狔犿狌狊狀狌狋犪狀狊)、ˆ ‰ Š (犌犲狀狋犻犪狀犪狊狋狉犪
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Analyzer)}œ DELTAVAdvantageŸ ¡è.
/ð*
(IsotopeRatioMassSpectrometer)wx”
•
,δ13Cz”á0»‘n0.1‰,δ15Nz”•0»‘
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δ13C(‰,VPDB)=[(犚 /ý/犚 ¤2)1]×
1000‰
δ15N(‰,atair)=[(犚/ý/犚¤2)1]×1000‰
t1 uvw67xyK67z{
Table1 Warmingamplitudesofopentopchamber

Height
st‘=
Warmingchamber

T/℃
0Vdæ
Airhumidity/%
U¶5cm
Underground5cm
CK
OTC1
OTC2
10.75a
11.55b
11.78c
U©5cm
Aboveground5cm
CK
OTC1
OTC2
10.41a
11.45b
11.63c
72.31a
74.7a
76.56a
U©20cm
Aboveground20cm
CK
OTC1
OTC2
10.20a
11.13b
11.32c
70.15a
73.27a
76.01a
  3:¾Ÿ‘456©í´µÂG0.05ÉÊãG¿Àž»¼。
  Note:Thedifferentnormallettersindicatesignificantdifference
amongtreatmentsat0.05level.
Ùd
,犚‹13C/12C715N/14N)*åæ­。
2 §¨NË-
2.1 犗犜犆K67|}
st‘=stÝënt=z89]ð

st:
=Ϲ;°±

0V<=͇

ú>Ö¾?@o

¹
Áèéê›VABCDdEpFGHzÓ]¬
I
[8],
g}=ÏtæÅR

ë©1Ð}JK,GU¶
5cm、U15cm U120cm,CK、OTC1、OTC2
ztæäwdæžíÅR

"ÛÙMS=LȖ
stz]ð

Æst‘=M‘

stzu¨Mª¿
(犘<0.05);OTCÏtæÅR,NùUO1Å>z
ÉËs¹

g}0Vdæi¸s¹
(犘>0.05)。I
J

stút=ϑVWôn¿ÀY>?

2.2 ~678=>?@犆/犖€KLM
š›ÜPÝ#$?,`zÜPòó

ëØ2Ð
JK

~T˜™zC/N­®GOTC1d­w¯°
±–14.1%,ÄGOTC2dQ­w¯s¹–8.3%;
‚ƒ„…T

†‡ˆ‰Š˜™z C/N ­®G
OTC1 OTC2z C/N ­®ËÌ­w¯s¹–
4.2%、19.4%1.1%,ÄG OTC2dRËÌ­w
¯s¹–8.1%、11.1%13.5%,º»¼½¾¿À
(犘>0.05)。ÐÑ,;$$˜™zC/N­®¸¿Àxy。
2.3 ~678=>?@i‚AEFGδ13犆€
KLM
  SØ3gí,G;#$˜™•žšŸ ¡δ13C®G-24.12‰~
-28.34‰ÁÂXY,ʽ®‹-26.78‰,(}~
Tδ13CÕÖ?±,ˆ‰Šδ13CÕÖ?R;~T˜™
9774Ø       DEE,¢:qrstwRST€#$˜™š›œ—Ÿ ¡δ13Cδ15NÕÖzxy
Ÿ$$Ï¾Ÿ‘456©í´µÂG0.05ÉÊãG
¿Àž»¼

¶Ÿ

Ⅰ.~T;Ⅱ.‚ƒ„…T;Ⅲ.†‡;Ⅳ.ˆ‰Š
Ø2 ¾Ÿ#$˜™C/N­®TstšæzXY
Thedifferentnormalletterswithinthesamespeciesindicate
significantdifferenceamongtreatmentsat0.05level;
Thesameasbelow.
Ⅰ.犓.犺狌犿犻犾犻狊;Ⅱ.犈.狀狌狋犪狀狊;Ⅲ.犗.狅犮犺狉狅犮犲狆犺犪犾犪;
Ⅳ.犌.狊狋狉犪犿犻狀犲犪
Fig.2 ThechangesofC/Nratioindifferentplantleaves
withthetemperaturegradients
Ø3 ¾Ÿ#$˜™δ13C®TstšæzXY
Fig.3 Thechangesofδ13Cindifferentplantleaveswiththe
temperaturegradients
δ13C®ÃstŠæÅRĞís¹,†‡、ˆ‰Š˜
™δ13C®ÃstŠæÅRÄží°±,ą
T˜™δ13C®R©%‹OTC1?±,—í‹OTC2,
º¾—d~TGOTC2z˜™δ13C®N—w¯Â
z»¼ÇÈ¿ÀÉÊ
(犘 <0.05)。—UNw¯»¼
½¾¿À
(犘>0.05)。IJ,;ÝÞ#$$˜™zδ13CÕÖ~láÌ¿Àxy。
2.4 ~678=>?@i‚BEFGδ15犖€
KLM
  Ø4¿í,G;$˜™•ž›Ÿ ¡δ15N ®G-4.67‰~
Ø4 ¾Ÿ#$˜™δ15N®TstšæzXY
Fig.4 Thechangesofδ15Nindifferentplantleaveswith
thetemperaturegradients
0.32‰ÁÂXY,ʽ®‹-1.95‰,(}~T
δ15NÕÖ?±,Ĉ‰Šδ15NÕÖ?R。—d,~
T˜™δ15N®GOTC1OTC2dz˜™δ15N
®ËÌ­w¯:R40.2%8.0%,ÆG OTC1d
ÇȖ¿ÀÉÊ
(犘<0.05);‚ƒ„…TG OTC1
OTC2dz˜™δ15N®­w¯ží°±,Ë̶
°–18.7%26.9%(犘>0.05);ˆ‰ŠGOTC1
OTC2dz˜™δ15N®­w¯žíÅR,ËÌs
¹–31.1%240.9%,ÆGOTC2dXY¿À(犘
<0.05);†‡GOTC1z˜™δ15N®­w¯°±
11.0%,ÄG OTC2z˜™δ15N ®­w¯:R
2.8%(犘>0.05)。ÐÑ,4RST€#$˜™
•ž›Ÿ ¡δ15N®wstzyv©%K¾Ÿz
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S

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>%st‘=Ïz0V
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NOën
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