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,2016,36(6):1091-1097
犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
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犆犾狅狀犻狀犵狅犳犪犖犲狑犌犲狀犲犜犪犇犚犔犲犪32犳狉狅犿犠犺犲犪狋犪狀犱
犐狋狊犈狓狆狉犲狊狊犻狅狀狌狀犱犲狉犇犻犳犳犲狉犲狀狋犛狋狉犲狊狊犲狊犜狉犲犪狋犿犲狀狋狊
LIANGYajun1,YUZhengyang1,QIANGZhiquan1,DUYa1,ZHU Weining2,
ZHANGDapeng1,ZHANGLinsheng1
(1ColegeofLifeSciences,NorthwestA&FUniversity,Yangling,Shaanxi712100,China;2ColegeofLifeSciences,North
westUniversity,Xian710069,China)
犃犫狊狋狉犪犮狋:Lateembryogenesisabundantprotein(LEA)areasuiteofimportantfamilyproteinsfoundinor
ganisms,whicharegeneralyinvolvedinosmoregulationandareexpressedabundantlywhensubjectedto
environmentalstresses.Inthisstudy,weisolatedanovel犔犈犃3gene犜犪犇犚犔犲犪32fromwheat‘Zhengyin
No.1’.Thefullengthof犜犪犇犚犔犲犪32is668bp,whoseORFis570bp,encoding189aminoacids,and
consistsof9conserved11merrepeatingmotifs;Proteinpredictionshowsthattheproteinishighlyhydro
philicandlocatedinthecytoplasm,withthreephosphorylationsitesandhasnotransmembranedomainor
signalpeptide;Secondarystructureanalysisshowsthatthemainstructureisalphahelix;Homologycom
parisonandregeneratingphylogenetictreewerecarriedoutbyMEGA5.1.Expressionanalysesrevealed
that犜犪犇犚犔犲犪32genewasinducedbydrought,lowtemperature,highsaltstressandalsomoreover,can
beinducedbyABAtreatment.Theseresultssuggestedthat犜犪犇犚犔犲犪32isanABAdependent犔犈犃3
gene,participatingintheresponseprocessofwheatundertheabioticstressesindifferentmechanismsand
canlayafoundationforelucidating犔犈犃3geneprotectionmechanismunderabioticstressesinwheat.
犓犲狔狑狅狉犱狊:wheat;犜犪犇犚犔犲犪32;homologicalcloning;sequenceanalysis;realtimePCR
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M.DNAMarker;1,2.PCRproductsof犜犪犇犔犲犪32gene;
3.PCRidentificationofpositiveplasmidpMD19T犜犪犇犚犔犲犪32
Fig.1 Cloneandidentifyof犜犪犇犚犔犲犪32
9µ11¤1¥¶·v¸¹äåé:8òK;<=
42 犜犪犇犚犔犲犪321¡¢v¤1¥äå
Ninerepeating11meraminoacidmotifsareunderlined
andnumbered
Fig.2 Theaminoacidsequenceof犜犪犇犚犔犲犪32
21¡¢vnoäå,®Í¨,犜犪犇犚犔犲犪321
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Fig.3 SequencealignmentofwheatTaDRLea32proteinandotherLEAproteins
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Fig.4 PhylogentictreeofwheatTaDRLea32proteinand
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A.SecondarystructureofwheatTaDRLea32protein.Theblue,red,greenandpurplelinesrepresentthealphahelix,
extendedstrand,betaturnandrandomcoil,respectively;B.TertiarystructureofwheatTaDRLea32protein.
Fig.7 Secondarystructure(A)andtertiarystructure(B)predictionofwheatTaDRLea32protein
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