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,2016,36(4):0667-0673
犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
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犪狀犱犕犪犻狀狋犪犻狀犲狉犔犻狀犲狊
ZHANGSini1,MAXiaofei2,ZHANGHongxia1,WANGZhen3,ZHANGZigang1,GUODong1,
ZHANGYongpeng1,MALingjian1
(1ColegeofAgronomy,NorthwestA&FUniversity,Yangling,Shaanxi712100,China;2WheatResearchInstituteofAgricul
turalSciences,Linfen,Shanxi041000,China;3NanyangAgriculturalScienceResearchInstitute,Nanyang,Henan473000,China)
犃犫狊狋狉犪犮狋:Ftypewheatmalesterilelineisanew “threelines”withkaryoplasminteraction.Forstudythe
polencharacteristicsofFtypesterilelineandscreeningitsrestorersandmaintainers,theI2KIstaining
methodisusedtoobservepolensofF,BNS,T,KandVtypewheatmalesterilelinesatbloomingstage,
andusing98hybridcombinationsthatFtypesterilelinewasfemaleand98qualitywheatlinesweremale
todetecttheselfseedsetratiosonF1ofcombinations.Resultsshowedthat:(1)polensterilityrateofF
typesterilelinereached95.63%includingthehighest67.66%stainedabortedrate,19.32%sphericala
bortedrateandonly8.73%typicalaborted.(2)ThepolensterilitycharacteristicsofFtypewasmostsim
ilartoKtype,thesecondwasVtypein5kindsofsterilelines.(3)In98hybridizedcombinations,11self
seedsetratios(international)weremorethan100%and18at0%-10%.(4)‘Zhoumai16’,M510,‘Xinong
815’and‘Xinong585’wereexcelentrestorerlinestoFtypesterileline.‘Tianmai989’,‘Cunmai4’,
CY5475,M460,‘12Luo1’and11GB02weregoodmaintainersforFtypesterilelinebybackcrosscultiva
tion.ResearchsuggeststhatFtypemalesterilelinehasthoroughandstablepolenabortionandeasily
soughtitsmaintainerresourcesandexcelentrestorersonfertility.
犓犲狔狑狅狉犱狊:wheat;Ftypesterileline;polenabortion;restorerandmaintainerline
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Differentlettersabovecolumnsindicatesignificantdifferenceamongsttrilelines(犘<0.05)
Fig.1 Polenfertilityanalysisoffivewheatsterilelines
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Table1 Variationonselfseedsetrateandpolentypes
offivewheatsterilelines
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½MvwFertilepolenrate 3.99 0.006 0.152
Selfseedsetratio 3.59 0.009 0.247
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Table2 Selfseedsetratioof31F1hybridizedcombinations(internationalalgorithm>100%ordomesticalgorithm<10%)
¯°
F1hybrid
/%
Domestic
±
/%
International
F1MK
F1fertility
¯°
F1hybrid
/%
Domestic
±
/%
International
F1MK
F1fertility
F(A)/´ I16Zhoumai16 83.82 134.15 Cf F(A)/M326 1.14 2.27 HS
F(A)/M510 92.17 149.41 Cf F(A)/M243 2.51 4.89 HS
F(A)/!a815Xinong815 83.35 115.32 Cf F(A)/ú 55Huagao55 7.95 10.23 HS
F(A)/!a585Xinong585 86.03 125.63 Cf F(A)/N0381 2.50 2.50 HS
F(A)/ºI2»Cunmai2 76.06 109.23 Hf F(A)/´ I24Zhoumai24 2.50 6.00 HS
F(A)/ºI8»Cunmai8 76.90 107.32 Hf F(A)/Hû18Xiaoyan18 3.41 3.41 HS
F(A)/13k|513Hexuan5 72.76 103.50 Hf F(A)/üI1» Guanmai1 6.67 11.94 HS
F(A)/13k|613Hexuan6 70.00 103.19 Hf F(A)/¹I989Tianmai989 0.00 0.00 CS
F(A)/07(52)098 63.16 107.24 Hf F(A)/ºI4»Cunmai4 0.00 0.00 CS
F(A)/M288 74.30 119.43 Hf F(A)/CY5475 0.00 0.00 CS
F(A)/!a165Xinong165 69.51 126.45 Hf F(A)/M396 0.00 6.25 CS
F(A)/ýI22Jimai22 1.09 2.17 HS F(A)/M460 0.00 0.00 CS
F(A)/!a223Xinong223 1.19 1.19 HS F(A)/11GC01 0.00 1.14 CS
F(A)/10ZB05 4.65 8.17 HS F(A)/11GB02 0.00 0.00 CS
F(A)/¢I895Zhongmai895 2.50 6.14 HS F(A)/12¼112Luo1 0.00 0.00 CS
F(A)/!a2208Xinong2208 1.04 2.08 HS
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Note:Relatedcoefficientbetweennationalandinternationalselfseedsetratioissignificantat0.01level;F(A)representsFtypesterile
line;Cf.Completefertility;Hf.Highfertility;HS.Highsterility;CS.Completesterility.
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:
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