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Expression of SbPRP3 in Soybean and Resistance Ability in Transgenic Tobacco to Abiotic Stress

大豆SbPRP3基因表达及转基因烟草非生物胁迫鉴定



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,2016,36(7):1331-1336
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ZHAIYing1,ZHANGJun2,YANGXiaojie1,ZHAOYan1,CHENYang1
(1ColegeofLifeScienceandAgroForestry,QiqiharUnivesity,Qiqihar,Heilongjiang161006,China;2HeilongjiangInstitute
ofVeterinaryScience,Qiqihar,Heilongjiang161005,China)
犃犫狊狋狉犪犮狋:Plantprolinerichproteins(PRPs)areputativecel walproteins,whichareusualyassociated
withdifferentabioticstresses.Theexpressionof犛犫犘犚犘3underabioticstresstreatmentsinsoybeanwas
detectedbyrealtimefluorescencequantitativePCR.Theexpressionof犛犫犘犚犘3increasedundersalttreat
ment,whileitincreasedfirstlyandthereafterdeclinedunderdroughtandcoldtreatments.Furthermore,
theORFof犛犫犘犚犘3wasclonedintotheplantexpressionvectorofpRI101ANandthenintroducedintoto
baccobyAgrobacteriummediatedtransformation.Threepositivetransgenictobaccoplantswereobtained.
Thetransgenictobaccoplantsweretreatedwithsalt,droughtandcoldstresses.Theresultsshowedthat
thetransgenicplantsmaintainedhigherlevelsofprolineandlowerlevelofmalondialdehydecomparedto
wildtypeplantsundersaltandcoldstresses.Whilethelevelsofprolineandmalondialdehydeintransgenic
plantshadnosignificantdifferencescomparedtowildtypeplantsunderdroughtstress.Thesedataindica
tedthatthetransgenictobaccoplantsconstitutivelyexpressing犛犫犘犚犘3showedanincreasedtoleranceto
saltandcoldcomparedtowildtypeplants.
犓犲狔狑狅狉犱狊:soybean;prolinerichprotein;犛犫犘犚犘3;expressionanalysis;stresstolerance
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treatments
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transgenictobaccoplantsaftersalttreatmentfor5h
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