作 者 ：李 俊，李建明，胡晓辉*，李之凯，王诗梦
期 刊 ：西北植物学报 2012年 9期 页码：1788~1795
Keywords:spermidine, tomato, salinity-alkalinity stress,
摘 要 ：以番茄耐盐碱品种‘金鹏一号’和盐碱敏感品种‘中杂9号’为试验材料，采用营养液水培的方法，设置正常营养液栽培（对照CK）、100 mmol/L盐碱溶液胁迫、100 mmol/L盐碱溶液胁迫+0.1 mmol/L亚精胺（Spd）浸种、100 mmol/L盐碱溶液胁迫+0.25 mmol/L Spd浸种4种处理，研究外源Spd对番茄幼苗株高和茎粗的相对生长率、叶片和根系含水量、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性以及丙二醛（MDA）、脯氨酸（Pro）、叶绿素含量的影响。结果显示：（1）单独盐碱胁迫下，两品种番茄幼苗叶绿素含量、株高和茎粗的相对生长率、叶片和根系相对含水量较对照均降低，而其MDA、Pro含量、SOD和POD活性显著增加。（2）外源Spd浸种处理较盐碱胁迫下能够增加番茄幼苗的株高和茎粗的相对生长率、叶片和根系的相对含水量、Pro及叶绿素含量，进一步提高SOD和POD的活性，降低其MDA含量，能有效缓解盐碱胁迫对番茄幼苗的伤害。（3）在100 mmol/L盐碱胁迫条件下，0.25 mmol/L Spd浸种处理对番茄幼苗受到的盐碱胁迫伤害的缓解效果更佳，并且这种缓解作用对盐碱敏感品种‘中杂9号’幼苗的效果更加明显。研究表明，在盐碱胁迫下，番茄幼苗的生长受到一定程度的抑制，但外源Spd浸种能够通过调节渗透调节物质含量、增强抗氧化酶活性来减轻盐碱胁迫的伤害，维持植物体的正常生理代谢功能，从而提高番茄幼苗抵抗盐碱胁迫能力，有效缓解盐碱胁迫的伤害，并以0.25 mmol/L Spd浸种处理缓解效果更佳，且这种缓解作用对盐碱敏感品种‘中杂9号’幼苗效果更明显。
Abstract:A hydroponic experiment was carried out with two different tomato cultivars in salinity-alkalinity tolerance to explore the effects of exogenous spermidine (Spd) soaking seeds on relative growth rates of plant height and stem diameter,contents of leaf water and root water,malondialdehyde (MDA),proline (Pro),chlorophyll,activities of superoxide dismutase (SOD) and peroxidase (POD).There are four processing:normal nutrient solution cultivation (CK),100 mmol/L salinity-alkalinity stress,100 mmol/L salinity-alkalinity stress+pre-soaked seeds with 0.1 mmol/L spermidine (Spd),100 mmol/L salinity-alkalinity stress+pre-soaked seeds with 0.25 mmol/L Spd.The results showed that:(1)The chlorophyll content,relative growth rate of plant height and stem diameter,water content in leaf and root reduced;the contents of MDA and Pro increased;the activities of SOD and POD enhanced under salinity-alkalinity stress.(2)Pre-soaked seeds with exogenous Spd could alleviate damage of salinity-alkalinity stress on tomato seedlings via increasing relative growth rates of plant height and stem diameter,water contents in leaf and root,reducing content of MDA,increasing contents of Pro and chlorophyll,enhanceing the activities of SOD and POD in tomato seedlings under stress condition.(3)Under 100 mmol/L salinity-alkalinity stress,0.25 mmol/L Spd has conspicuous impact on alleviating the harm of tomato seedlings,which was more significant for sensitive salinity-alkalinity ‘Zhongza No.9’ seedlings.In summary,the growth of tomato seedlings by a certain degree of inhibition under salinity alkalinity stress,but exogenous Spd could keep tomato seedlings maintain the normal physiological metabolism and improve the seedling resistance to salinity-alkalinity stress ability by adjusting osmotic adjustment material contents and increasing antioxidant enzyme activities to alleviate damage caused by salinity-alkalinity stress.0.25 mmol/L exogenous Spd could enhanced tolerance of tomato plants to salinity-alkalinity stress and this relieve effects on ‘Zhongza No.9’ seedlings is obvious.