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作 者 ：丁 可，汪晓峰*

期 刊 ：西北植物学报 2012年 3期 页码：439~446

关键词：甘蓝型油菜；水苏糖合成酶；基因克隆；表达分析；

Keywords:Brassica napus L., stachyose synthase, gene clone, gene expression,

摘 要 ：以甘蓝型油菜品种‘德油5号’为试材，采用RT-PCR和RACE方法首次从油菜中获得水苏糖合成酶基因cDNA全长序列，命名为BnSTS，GenBank登录号为HQ285880.1。该cDNA全长3 210 bp，包含一个长为2 619 bp的开放阅读框，编码873个氨基酸。通过构建原核表达载体，成功表达出预期蛋白。同源性比对结果显示，该基因编码蛋白与拟南芥STS相似性最高为82.08%。荧光定量PCR结果显示，基因表达水平与油菜种子发育过程中水苏糖含量变化具有相关性，与种子脱水耐性的获得具有时间一致性。该基因可能在种子脱水耐性形成机制中具有一定的作用。

Abstract:In this study,a full-length cDNA sequence of stachyose synthase gene was obtained from Brassica napus cultivar ‘Deyou 5’ for the first time using RT-PCR and RACE,named BnSTS (GenBank accession No.HQ285880.1).The full length of BnSTS is 3 210 bp,and the ORF is 2 619 bp,encoding 873 amino acids.Prokaryotic expression showed the recombinant plasmid can express the prospective proteins.Homology analysis of amino acid sequence of BnSTS showed the highest 82.08% similarity with STS from Arabidopsis thaliana.The Real-Time PCR indicated that gene expression level and the concentration of stachyose in different development stage of seeds were associated.Gene expression and the acquirement of desiccation tolerance of seeds had a time consistency trend,suggesting that the gene may play an important role in seed desiccation tolerance mechanisms.

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