作 者 ：张艳玉，张卫东，高庆荣*，张保雷，李楠楠，高建华，王慧娜，赵兰飞
期 刊 ：西北植物学报 2013年 6期 页码：1165~1170
Keywords:wheat, thermo-sensitive male sterility (TGMS), endogenous plant hormones,
摘 要 ：为探讨温光敏雄性不育小麦BNS育性与内源激素的关系，该研究以BNS不育株和可育株的叶片、幼穗和花药为研究材料(以鲜重计)，在雌雄蕊分化期(Ⅰ)到三核期(Ⅵ)之间分期取材。采用酶联免疫法测定生长素（IAA）、赤霉素（GA）和脱落酸（ABA）的含量。结果显示：（1）在发育过程中，不育株IAA含量（33~95 ng/g）显著低于可育株（57~112 ng/g）；不育株ABA、GA含量分别为48~129 ng/g、3~14 ng/g，可育株分别为77~132 ng/g、4~11 ng/g，大部分时期不育株显著低于可育株。（2）在雌雄蕊分化期，幼穗不育株IAA和GA含量分别为33.85 ng/g和7.13 ng/g，可育株分别为50.55 ng/g和11.84 ng/g，不育株IAA和GA含量均显著低于可育株。（3）在幼穗发育期内，不育株IAA/ABA、IAA/GA和ABA/GA分别为0.4~1.1、4.3~15和7~20，可育株为0.5~0.9、4.3~11.5和7.3~13，与可育株相比，不育株中激素比例浮动较大，表现比例失调。研究表明，BNS幼穗发育中激素的比例失调是雄性不育的形成原因之一，而雌雄蕊分化期的IAA、GA的含量下降可能促进BNS雄性不育的发生。
Abstract:In order to study elucidate the relationship between fertility development and endogenous hormones in thermo-photo-sensitive male sterile wheat line BNS,we toke leaves,young panicles and anthers from BNS fertile plants and sterile plants as experimental materials (fresh weight) in this study and they were collected through the stage of pistil and stamen differentiation (Ⅰ) to the trinuclear stage (Ⅵ).And the contents of auxin,abscisic acid and gibberellin in leaves,young panicles and anthers from sterile and fertile lines were detected using indirect ELISA methods.The results are as follows:(1)As the sterile line development,the hormones changes were different from those in fertile line.The IAA contents in sterile and sterile lines were 33~95 ng/g and 57~112 ng/g respectively through the development stages.The IAA contents in ferile line were lower than those in fertile line (P<0.05).The contents of ABA and GA in sterile line displayed irregular changes compared with those in fertile line.The contents of ABA and GA in sterile line were 48~129 ng/g and 3~14 ng/g respectively in sterile line,and those in fertile were 77~132 ng/g and 4~11 ng/g,respectively.(2)At pistil and stamen differentiation stage,the contents of IAA and GA in young panicles from sterile line were 33.85 ng/g and 7.13 ng/g,and those from the fertile line were 50.55 ng/g and 11.84 ng/g,respectively.The contents of the respective hormones from different lines were highly significant differences (P<0.01).(3)Through the development stages,the ratios of IAA/ABA,IAA/GA and ABA/GA in sterile line were 0.4~1.1,4.3~15 and 7~20 respectively,and those in fertile were 0.5~0.9,4.3~11.5 and 7.3~13 respectively.Compared with those in fertile line,the ratios of hormones in sterile line displayed more changeable and were be out of proportion through the developmental stages.These results indicated the unbalanced ratios of hormones might be one of the causes that lead to male sterility,more specially,the decreased levels of IAA and GA in pistil and stamen differentiation stage might promote the occurrence of male sterility in BNS.