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作 者 ：安洪周,胡 梅,郑会敏,张素勤*,张庆勤

期 刊 ：西北植物学报 2013年 2期 页码：295~300

关键词：小麦；野燕麦；减数分裂；染色体行为；基因组原位杂交；

Keywords:Triticum timopheevi, Avena fatua L., meiosis, chromosome behavior, GISH,

摘 要 ：通过普通细胞学和基因组原位杂交（GISH）研究提莫菲维小麦（Triticum timopheevi）与光稃野燕麦（Avena fatua L.var.glabrata Pat）远缘杂交后代花粉母细胞染色体减数分裂行为。结果表明,该F₃株系花粉母细胞的减数分裂指数为87.46,表现出一定的遗传不稳定性。GISH分析发现该F₃株系中期I细胞中有4对红色杂交信号,且游离的单价体上各有1个杂交信号;后期Ⅰ、后期Ⅱ和末期Ⅱ出现落后染色体、染色体桥、微核等现象,分别占观察数的10.58%、1.92%和12.36%,这些异常现象可能是由于光稃野燕麦遗传物质干扰了小麦同源染色体或部分同源染色体的正常配对造成的。

Abstract:The chromosomic meiotic behavior of pollen mother cells (PMCs) of the hybrid progenies from Triticum timopheevi×Avena fatua L.var.glabrata Pat was researched by general cytology and genomic in situ hybridization (GISH) in the present study.The results showed that the PMCs meiotic index of the F₃ line was 87.46,indicating some genetic instability.4 pairs of red hybridization signals were found in the PMCs at the metaphase I by GISH analysis,and there was a red signal in each dissociative univalent.Some lagging chromosome,chromosome bridges and micronuclei were discovered at the anaphase Ⅰ,anaphase Ⅱ and telophase Ⅱ,with the percentage of 10.58%,1.92% and 12.36%,respectively.Wheat homologues or homoeologues normally pairing was interfered by the wild oat chromatin might lead to these abnormal phenomena.

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