Abstract:Antigenic membrane proteins(AMPs) encoded by phytoplasma play important roles in recognition between pathogen and host.To study the function of AMP of Paulownia witches′broom(PaWB) phytoplasma and detect the pahogen,specific primers were designed and used for amplification of the amp gene.The sequencing results showed that the open reading frame(ORF) of amp gene consisted of 696 nt and encoded 231 amino acid residues,which were 100% identical to two other sequenced amp genes of PaWB phytoplasma.For antibody preparation,the partial sequence of amp from nt 91 to 604 was cloned into the expression vector pGEX-4T-3.After induction by IPTG,the total protein was separated by SDS-PAGE,which showed that the fusion protein was overexpressed in E.coli.The antiserum against the fusion protein was raised in rabbit and applied specifically for detection of PaWB phytoplasma in Western blot,dot blot,ELISA,indirect immunofluorescence and immunocapture PCR assays.