Abstract:In order to study the effect of different sulfur and nitrogen fertilizer combined application on N transfer and leaf senescence of winter wheat,N content、water and chlorophyll content of 3 winter wheat varieties (‘Yumai 66’ and ‘Weimai 8’ are stay green wheat,‘Xiaoyan 6’ is non stay green wheat) were measured.The results showed that under N120 treatment,N content of stem and leaf,chlorophyll content of leaf with S60 treatment was lower than that of other two S treatment at anthesis;Leaf water content was lower than that of other two S treatment at the late grain filling stage.Under N220 treatment,leaf N content at anthesis,total N accumulation at harvest,N Harvest Index,water content and chlorophyll content of leaves were all higher than that of other two S treatment.The effect of sulphur on wheat depended on the amount of nitrogen application,and S demonstrated a dual character in all 3 winter wheat varieties.On the one hand,under the condition of lower level nitrogen,sulphur had negative effect on the N uptake and senescence of leaf;On the other hand,under the condition of higher level nitrogen,sulphur had positive effect on the N uptake,senescence of leaf,grain yield and total biomass.The appropriate amount of N application for high yield was about 220 kg/hm2,and the appropriate amount of S application for high yield was about 60 kg/hm2.High level N and S fertilizer delay senescence more in non stay green wheat than that in stay green wheat.