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作 者 ：艾育芳1，陈观水1，周以飞2，张 梅1,3，潘大仁1*

期 刊 ：西北植物学报 2012年 10期 页码：1965~1970

关键词：油菜；早熟；成花；BnLFY基因；克隆；序列分析；

Keywords:Brassica napus L., early maturity, flowering, BnLFY gene, cloning, sequence analysis,

摘 要 ：以甘蓝型油菜晚熟品种RG-8的早熟突变体RG-8M为材料，通过同源克隆法得到1个LEAFY（LFY）同源基因，命名为BnLFY。该基因cDNA全长为1 310 bp，包含1个长为1 248 bp的开放阅读框，编码415个氨基酸。序列分析表明，推测的氨基酸序列含双子叶植物LFY类蛋白特有的N端脯氨酸富集区、中央酸性区、亮氨酸拉链结构以及富含赖氨酸与精氨酸的碱性区；且与几种十字花科植物LFY类蛋白的氨基酸序列一致性均在84%以上。转录表达分析表明，BnLFY基因在油菜中为组成型表达。

Abstract:RG-8M,early-maturing mutant of late-maturing rape RG-8,was used in the present study.A LEAFY（LFY） homologue gene named BnLFY was cloned from RG-8M with the method of homologous cloning.The full length of BnLFY cDNA was 1 310 bp and contained a complete open reading frame of 1 248 bp,which encoded 415 amino acids residues.As other dicotyledon LFY-like proteins,the deduced amino acid sequence of BnLFY gene had several special regions such as N terminal proline rich region,the central acidic domain,the leucine zipper structure and the lysine and arginine-rich basic region.The result of sequencing indicated that the BnLFY protein shared more than 84% amino acid sequence identity with LFY-like proteins of several cruciferous plants.Transcriptional analysis showed the BnLFY gene expressed constitutively in rape.

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