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作 者 ：侯晓婉1,2,胡 伟2,颜 彦2,徐碧玉2,金志强2*

期 刊 ：西北植物学报 2015年 3期 页码：427~433

关键词：水通道蛋白；PIP；香蕉；克隆；荧光定量PCR；亚细胞定位；

Keywords:aquaporins(AQP), PIP, banana, cloning, quantitative RT-PCR, subcellular localization,

摘 要 ：该研究从香蕉中克隆了一个水通道蛋白(AQP)基因MaPIP2-6。序列分析表明,MaPIP2-6基因开放阅读框(ORF)为849 bp,编码282个氨基酸。多序列比对和进化树分析表明,MaPIP2-6基因所编码的蛋白与其它植物中AQP蛋白具有较高的一致性,并且与水稻OsPIP2-6的亲缘关系最近。亚细胞定位表明,MaPIP2-6基因定位在细胞膜上。实时荧光定量PCR分析表明,甘露醇和高盐胁迫处理下,MaPIP2-6基因在巴西蕉和粉蕉中的表达趋势基本一致,在处理早期表达量轻微下降,随后被诱导并达到最大值,然后下降;在低温和ABA处理下,MaPIP2-6基因在巴西蕉和粉蕉的表达趋势相反,低温处理47 h时,巴西蕉的MaPIP2-6表达量显著降低,而粉蕉无显著变化,但在其他时间点,巴西蕉的表达量无显著变化,粉蕉显著降低。ABA处理下,MaPIP2-6基因在巴西蕉被诱导,而在粉蕉被抑制。研究认为,MaPIP2-6可能参与了非生物逆境胁迫应答,为进一步研究MaPIP2-6基因的功能鉴定了基础。

Abstract:In the present study,we isolated an aquaporins gene designated MaPIP2-6 from banana.MaPIP2-6 ORF is 849 bp,which encodes 282 amino acids.Comparison of amino acid sequences and phylogenetic analysis indicated that MaPIP2-6 was closely related with OsPIP2-6.Subcellular localization assays showed that the MaPIP2-6 protein was present in the plasma membrane.Real-time quantitative polymerase chain reaction (QPCR) assay revealed that the expression of MaPIP2-6 was consistent after treatment of mannitol and NaCl,a slight decline in the early treatment,and then was induced and reach the maximum,then decreased,but was inverse after treatment of cold and ABA,and that MaPIP2-6 participated in the response of abiotic stresses at transcriptional level.These results indicated that MaPIP2-6 may be involved in abiotic stress response,which lays a foundation for further investigating the function of MaPIP2-6.

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