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作 者 ：吕世博,毛昱力,姜廷波,马旭俊*

期 刊 ：西北植物学报 2015年 3期 页码：434~439

关键词：毛果杨；组蛋白去乙酰化酶；克隆；盐胁迫；基因表达；

Keywords:Populus trichocarpa, histone deacetylase, clone, salt stress, gene expression,

摘 要 ：该实验克隆了毛果杨组蛋白去乙酰化酶基因HDA901的编码序列,并进行生物信息学、亚细胞定位和盐胁迫表达分析。序列分析表明,HDA901开放阅读框为1 245 bp,编码1个由414个氨基酸残基组成的蛋白质,等电点为5.77;毛果杨HDA901与其他植物同源蛋白具有一段保守序列,在进化上与拟南芥AtHDA14亲缘关系较近。启动子分析表明,毛果杨HDA901基因启动子序列包含ACE、ABRE、HSE 和TC-rich repeats 等多个与逆境相关的顺式作用元件。亚细胞定位分析表明,毛果杨HDA901蛋白在细胞核和细胞质中无分布,可能位于线粒体或穿梭于线粒体和叶绿体之间。实时荧光定量PCR结果显示,毛果杨HDA901基因表达受盐胁迫调节,在盐胁迫下,根和茎中HDA901基因表达受抑制;叶中HDA901基因表达受诱导。研究表明,毛果杨HDA901基因参与盐胁迫应答反应。

Abstract:The Open Reading Frame(ORF) sequence of a histone deacetylase gene,named HDA901,was cloned from Populus trichocarpa and its sequence,sub-cellular localization and expression under salt stress were analyzed.The ORF of HDA901 is 1 245 bp,encoding a protein consisting of 414 amino acids.The isoelectric point of HDA901 protein is 5.77.Sequence alignment and phylogenetic analysis showed that HDA901 shared a conserved domain with the HDACs in other plants and was relatively close to Arabidopsis AtHDA14 in evolution.Promoter sequence analysis showed that the promoter of HDA901 gene contained multiple stress-related cis-acting elements including ACE,ABRE,HSE and TC-rich repeats.Sub-cellular localization analysis showed that HDA901 was localized neither in nucleus nor in cytoplasm,but might be localized in mitochondria and(or) shuttle between mitochondria and chloroplasts.Real-time PCR analysis showed that the expression of HDA901 gene was in response to salt stress.Under the salt stress,the expression of HDA901 was down-regulated in stem and root,while was up-regulated in leaf.These findings indicated that HDA901 was involved in salt stress response.

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