---

作 者 ：于月华，王莉萍,高文伟,陈全家,耿洪伟,张巨松,曲延英*

期 刊 ：西北植物学报 2012年 6期 页码：1073~1078

关键词：小麦；MYB类转录因子；基因克隆；表达分析；

Keywords:wheat, MYB transcription factor, gene cloning, expression analysis,

摘 要 ：采用RT PCR方法,从小麦中克隆获得1个盐诱导小麦MYB类转录因子基因TaSIM（Triticum aestivum salt induced MYB），该基因cDNA全长1 213 bp，具有1个831 bp的开放阅读框，编码276个氨基酸，预测分子量约为29.903 kD，等电点为10.12，推测的氨基酸序列中含有2个高度保守的SANT结构域。系统发生树分析表明，TaSIM与二穗短柄草XP_003576185亲缘关系最近。半定量RT PCR检测结果显示，TaSIM基因受盐胁迫诱导表达。亚细胞定位结果显示，TaSIM hGFP融合蛋白定位于细胞核中。研究结果表明，小麦TaSIM基因编码的蛋白可能在细胞核内参与小麦对盐胁迫的应答反应。

Abstract:In this study,a salt induced MYB transcription factor gene,TaSIM,was isolated from cDNAs library of wheat leaves under salt stress.The full length TaSIM cDNA is 1 213 bp,including an ORF of 831 bp.This cDNA sequence encoded a polypepide of 276 amino acid residues with a predicted molecular mass of 29.903 kD and a basic isoelectric point of 10.12,and two highly conserved SANT domain in the encoded putative protein.The phylogenetic analysis showed that TaSIM has the highest similarity with XP_003576185 from Brachypodium distachyon.Semi quantitative RT PCR analysis revealed that TaSIM gene was obviously induced by salt stress.Subcellular localization assay indicated the TaSIM hGFP fusion protein was located in the nucleus.The results suggested that TaSIM transcription factor might be involved in salt stress response of wheat.

---