Abstract:The kinetic parameters of chlorophyll fluorescence and the photosynthetic rate of the different genetic background flue-cured tobacco varieties (‘K326’,‘Yunyan 87’,and ‘Zhongyan 103’) were studied under different altitude conditions,and their influencing factors Gs,Ci,Tr,WUE were investigated.The result showed that:(1)There were positive correlation between the average daily value of Pn,Gs,WUE and altitude and negative correlation between the average daily values of intercellular CO2 concentration (Ci),transpiration rate (Tr) and altitude.(2)The diurnal variation of Pn,Ci,Gs,and Tr in the leaves presented a one-peak distribution and an unobvious midday depression occurred at altitude of 1300 m,and the diurnal variation of Pn,Ci,Gs,and Tr presented a bimodal pattern and an obvious midday depression occurred in the sunny noon.(3)The primary light energy conversion of PSⅡ (Photo-systemⅡ) (Fv/Fm) and potential activities of PSⅡ(Fv/F0) of the three tobacco varieties increased with increasing altitude.(4)The daily photosynthesis (∑Pn) of the three tobacco varieties increased with increasing altitude,and the daily photosynthesis of ‘Yunyan 87’ varieties were significantly higher than the other two varieties.At altitude of 900 m,flue-cured tobacco’s leaves had the highest daily transpiration (∑Tr),and the lowest in 1 300 m.Obviously,the photosynthetic characteristics and photosynthetic capacity of flue-cured were affected and restricted by altitude factors,and the photosynthetic characteristics of tobacco varieties should be considered during distribution of varieties.