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作 者 ：徐 菲，宣继萍，郑玉红，刘永芝，郭忠仁*

期 刊 ：西北植物学报 2012年 3期 页码：471~476

关键词：秋海棠属；ISSR；种质资源；亲缘关系；

Keywords:Begonia, ISSR, germplasm resourse, genetic relationship,

摘 要 ：应用ISSR标记方法对秋海棠属（Begonia L.） 植物33个种的种质亲缘关系进行分析，从70条引物中筛选出13个多态性引物，并通过POPgen 32软件以UPGMA法分析其亲缘关系。结果表明：33份秋海棠属植物的遗传距离在0.188 9~0.932 8之间，多态性百分率达到97.54％，Shannon信息指数为0.551 9，Nei基因多样性指数为0.376 0。秋海棠属植物具有丰富的遗传变异，UPGMA聚类分析结果与现有属下分类学表现较为一致，且生态地理条件相似的种群优先聚集。

Abstract:ISSR (inter-simple sequence repeat) molecular markers were employed to investigate the genetic relationship among 33 species of Begonia.We chose 13 polymorphic primers from 70 primers to analyze their genetic relationships with popgen32 software.UPGMA results showed that the genetic distances among the 33 Begonia species were between 0.188 9 and 0.932 8,the polymorphism percentage is 97.54% and the Shannon diversity index was 0.551 9,the Nei’s gene diversity index was 0.376 0.With their rich genetic variations,the UPGMA analysis goes well with the recent classifications.With similar ecological and geographical conditions,the populations clusters firstly.

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