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作 者 ：刘珠琴1，2，吴巨友1，姜雪婷1，徐国华1，3，张绍铃1*

期 刊 ：西北植物学报 2012年 9期 页码：1763~1767

关键词：梨；花粉管；微丝骨架；钙离子通道；细胞松弛素B；鬼笔环肽；

Keywords:pear, pollen tube, microfilament cytoskeleton, calcium ion channels, cytochalasin B, phalloidin,

摘 要 ：应用激光共聚焦显微镜和全细胞膜片钳技术研究了微丝骨架解聚剂细胞松弛素B（CB）和稳定剂鬼笔环肽（PD）对梨花粉管细胞内钙离子浓度动态变化和尖端质膜上钙离子通道的影响。结果显示：CB处理能促进花粉管内胞质钙离子[Ca²⁺]_i浓度增加，同时还能激活质膜上的钙离子通道；而PD处理对花粉管内[Ca²⁺]_i浓度及钙离子通道几乎没有影响。研究表明，微丝骨架的解聚激活了花粉管质膜上的钙离子通道，使得胞外钙离子大量流入，胞内钙离子浓度升高，从而抑制花粉管生长。

Abstract:Laser confocal scanning microscope (LCSM) and whole-cell patch clamp were used to reveal the influence of actin cytoskeleton deploymerizing agent cytochalasin B （CB）and stabile reagent phalloidin (PD) on the dynamic of cytosolic free calcium concentration ([Ca²⁺]_i) and calcium ion channels in the protoplasm membrane of Pyrus pyrifolia pollen tube cutting-edge.The results showed that CB could promote [Ca²⁺]_i increase and activate Ca²⁺ channel in plasma membrane;while phalloidin had no effect on [Ca²⁺]_i and did not activate Ca²⁺ channel.The results indicated that disruption of actin cytoskeleton might inhibit pollen germination and tube growth by activating Ca²⁺ channel in plasma membrane and increasing the concentration of [Ca²⁺]_i.

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