Abstract:In this study,the comparative fluorescent quantitative real-time PCR is used to study the expression of CCR gene in ovules,testas（from 10 to 60 days after pollination）,leaves,stems,chapiters and other organs of hull and hull-less Cucurbita pepo.The results indicated that:(1)The expression of CCR gene in both samples showed very similar pattern:raise-decline-raise-decline in hull and hull-less testa during the stage of ovule to 60 days after pollination and the expression levels of CCR gene have significant and highly significant difference between periods of pumpkins.(2)The expression level of CCR in hull-less was 1.2 fold higher than that in hull pumpkin ovules.(3)Follow the developmental stages,the expression levels of CCR gene in hull pumpkin’s testas were always higher than that of hull-less pumpkin.During the period of 10 to 60 days after pollination,the expression levels in the hull pumpkin testas were from 1.2 to 5.1 folds higher than those of the hull-less pumpkin.It suggested that the CCR gene was not only take part in the development process of testa but also played roles on the formation of the seed coat.The expression levels of CCR gene in hull-less pumpkin leaves,stems,chapiters and other organs are higher than those of the hull pumpkin,and the results also showed that the expression pattern of CCR gene has tissue-specific characteristics.The expression of CCR gene positively associated with the lignified degree of organs.