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作 者 ：尹中明  周永国  徐申中  李建粤

期 刊 ：西北植物学报 2007年 10期

关键词：水稻；Anti-Waxy基因；外源基因拷贝数；杂交；糙米直链淀粉含量；

Keywords:rice, Anti-Waxy gene, the transgenic copy number, hybrid, amylose content,

摘 要 ：将含单拷贝Anti-Waxy基因的纯合植株分别作为父本或母本进行正反交,获得两组含双拷贝Anti-Waxy基因的杂交后代,分析了未转基因对照、转基因亲本及两组杂交后代糙米直链淀粉含量以揭示不同Anti-Waxy基因拷贝数对降低稻米直链淀粉含量程度的影响.结果显示,2个单拷贝转基因水稻糙米直链淀粉平均含量分别为10.72%和11.13%,比对照分别降低17.98%和14.84%;两组正交和反交杂交后代糙米直链淀粉平均含量分别为8.96%和8.23%,其平均值为8.60%,比2个转基因杂交亲本糙米直链淀粉含量分别降低19.78%和22.73%,比对照降低了34.20%.结果表明,增加转基因水稻基因组中Anti-Waxy基因拷贝数在一定程度上能够进一步降低稻米直链淀粉含量,通过将独立转化获得同品种转基因植株之间杂交可以成为获得高表达转基因植物的途径.

Abstract:Experiments were conducted to investigate the reductiong of amylose contents in transgenic rice with different copies of Anti-Waxy.In this study,two different homozygotes with single copy of Anti-Waxy were used as male or female parent to conduct reciproc

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