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作 者 ：罗成科  彭正松  陈卫民

期 刊 ：西北植物学报 2006年 4期

关键词：半夏；悬浮培养；体细胞胚胎发生；植株再生；

Keywords:Pinellia ternata, suspension tissue culture, plant regeneration,

摘 要 ：用三叶半夏幼嫩叶片诱导产生的胚性愈伤组织建立了胚性细胞悬浮系,研究了悬浮培养下体细胞胚胎的发生及植株的再生。结果表明,胚性悬浮细胞在附加1．0mg／L2,4-D、0．2 mg／LBA和300mg／L LH的MS液体培养基中产生大量的球形胚,转入液体分化培养基(MS 0．1 mg／LNAA 0．2 mg／L BA 300 mg／L LH)中进一步发育成心形胚、鱼雷形胚和子叶形胚。收集成熟胚转移到MS固体分化培养基上培养获得了再生植株。另外还观察到某些成熟胚上产生了许多次生胚。

Abstract:The embryonic callus that young and tender Pinellia ternata leaves were induced to produce were used to establish embryonic suspension lines and the embryogensis and plant regeneration of somatic cells in suspension tissue culture were studied with the li

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