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作 者 ：张红  张艳敏  陈晓流  陈学森

期 刊 ：西北植物学报 2008年 5期

关键词：欧李；S基因；李属分类；

Keywords:Prunus humilis, S gene, taxonomy of Prunus,

摘 要 ：以6株欧李优选株系为实验材料,利用引物EM-PC2consFD和EM-PC3consRD对欧李进行S等位基因的专一性PCR扩增、克隆测序,获得了9个大小不同的序列,经BLAST分析确定为欧李的9个新S基因,分别命名为S1~S9,GenBank登录号依次为EF569602、EF569603、EF577404、EF577405、EF595836、EF595837、EF601047、EF653137、EF653138,并确定了6株欧李优选株系的S基因型。依据欧李的9个新S基因和李属其它S基因构建系统树,聚类结果显示李、杏、梅、欧李、扁桃及甜樱桃种间相互交叉,表明李属果树的S基因起源于共同的祖先;李、杏、梅、扁桃及甜樱桃应归为一个属,即李属;并初步探讨了欧李的分类地位。

Abstract:S-genotypes of six Prunus humilis accessions native to China were detected using S-allele-specific PCR with primer combinations EM-PC2consFD EM-PC3consRD.Nine S-RNase alleles were identified and were defined as S1~S9.S1~S9 were aligned in GenBank by BLAST

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