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作 者 ：詹园凤  吴震  金潇潇  王广东

期 刊 ：西北植物学报 2006年 9期

关键词：大蒜(AlliumsativumL.)；体细胞胚胎发生；超氧化物歧化酶；过氧化物酶；过氧化氢酶；

Keywords:garlic(Allium sativum L.), somatic embryogenesis, superoxide dismutase, peroxidase, catalase,

摘 要 ：以大蒜的发芽叶基(鳞茎)为外植体诱导体细胞胚胎发生,研究大蒜体胚发生过程中SOD、POD和CAT 3种抗氧化酶的活性以及可溶性糖和可溶性蛋白质含量变化.结果表明:在大蒜体胚发生过程中,SOD、POD和CAT活性变化与胚性愈伤组织的诱导及体胚的发育密切相关,POD对体胚的诱导起主导作用,而SOD和CAT在体胚的发育和成熟中起主导作用.可溶性糖和可溶性蛋白质累积与大蒜体细胞胚胎发生密切相关.

Abstract:As the explants,the leaf bases of germinating buds of garlic were induced to produce somatic embryos and SOD,POD and CAT activities and soluble sugar and protein contents in somatic embryogenesis were examined.It was shown that in somatic embryogenesis of

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