---

作 者 ：曾维英  杨守萍  喻德跃  盖钧镒

期 刊 ：西北植物学报 2008年 8期

关键词：大豆；质核互作雄性不育；双向凝胶电泳(2-DE)；蛋白质；

Keywords:soybean, cytoplasmic-nuclear male-sterility, two-dimensional gel electrophoresis(2-DE), protein,

摘 要 ：采用双向凝胶电泳技术对大豆质核互作雄性不育系NJCMS2A及其保持系NJCMS2B的种子、叶片和花药等不同器官蛋白质进行比较分析.结果显示,不育系NJCMS2A与其保持系NJCMS2B的花药2-DE图谱间存在较多差异表达蛋白点,种子2-DE图谱间仅有少量差异表达蛋白点,而叶片2-DE图谱间基本没有差异表达蛋白点.结果表明,不育基因表达具有时空性和器官特异性,与育性有关的蛋白主要在花药中表达.

Abstract:Two-dimensional gel electrophoresis(2-DE) technique was used to conduct the comparative protome study of different organs such as seeds,leaves,and anthers between the cytoplasmic-nuclear male-sterile line NJCMS2A and its maintainer NJCMS2B in soybeans.The

---