作 者 :郭卫东 郑学勤
期 刊 :西北植物学报 1999年 3期
关键词:抗旱;LEA;cDNA;植物表达载体;
Keywords:drought tolerance, LEA cDNA, plant expression vector,
摘 要 :在克隆了二棱大麦第3组LEAcDNA,抗旱基因HDCS1的基因上,将其连接于pB1121的CaMV35S启动子和NOS终止子之间,,构建了HDCS1的植物表达载体pBHC,并进行了PCR和酶切鉴定,为进行植物抗旱基因工程研究创造了条件。
Abstract:Based on the cloning of a drought tolerant gene HDCS1 ,which is one of the group 3 LEA cDNA,a plant expression vector pBHC was constructed for this gene.In pBHC the fragment of HDCS1 was ligated downstream to CaMV