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The Effective Components Extraction and Antifungal Test for the Heartwood of Platycladus orientalis

侧柏心材活性成分的提取及其抑菌性能


摘 要: 研究侧柏心材不同部位及正己烷和二氯甲烷依次提取的心材活性成分对白腐菌、褐腐菌、黑曲霉菌的抑菌作用,并采用GC/MS联用技术对提取物中的活性成分进行鉴定和分析。结果表明:侧柏心材从髓心向边材根据年轮所划分的3个部位对3种真菌都具有较好的抑菌效果,并且靠近边材的2部分抑菌效果更明显些。气质联机技术对正己烷和二氯甲烷提取物分析鉴定表明:侧柏心材正己烷提取物中有14种化合物,大部分化合物属于萜烯类物质及其衍生物,最高含量化合物为罗汉柏烯 (25.564%)和雪松醇(19.743%); 侧柏心材二氯甲烷提取物中鉴定出24种化合物,最高含量化合物也为罗汉柏烯(15.429%)。侧柏心材正己烷提取物对褐腐菌、白腐菌和黑曲霉菌第14 d时的抑菌圈直径分别为10.0,93和 8.3 mm,大于二氯甲烷提取物抑菌圈直径(0,2.7和1.3 mm),表明正己烷提取物对3种真菌的抑制生长作用明显优于二氯甲烷提取物的抑菌作用。

Abstract: The antifungal effects on different position of Platycladus orientali heartwood and the sequent extractives of hexane and dichloromethane heartwood were carried out with whiterot fungi, brownrot fungi and aspergillus niger, respectively. The chemical components of the two kinds of heartwood extractives were also identified and analyzed by use of GC/MS. The antifungal tests showed that the heartwood powder from pith to sapwood according to the growth rings had notable antifungal effects on the three kinds of fungi mentioned above.Furthermore, the antifungal effect of the two\|part heartwoods nearby sapwood was better than that of the heartwood nearby the pith. 14 kinds of chemical components were identified from the heartwood extractives by GC/MS, most of which are terpenoids and their derivatives, and the content of thujopsene is the highest (25.564%), cedrol (19.743%) is second only to that of thujopsene. 24 kinds of chemical components were identified from the dichloromethane extractives, and the highest component is also thujopsene (15.429%). The antifungal growth diameter of the hexane heartwood extractives on the 14th days was respectively 10.0, 9.3 and 8.3 mm, which was larger than the dichloromethane ones for brownrot fungi, whiterot fungi and aspergillus niger. It is proved that hexane extractives have a good inhibition effect on the three fungi growth than dichloromethane extractives.


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