摘 要:分别对黄山店欧李进行25 d的高浓度NaHCO 3溶液处理、干旱处理以及碳酸盐与干旱共同处理,测定胁迫期间该物种各理化指标的变化,研究欧李对不同环境胁迫的抗逆机制。结果表明: 欧李的叶绿素a、丙二醛和脯氨酸含量在不同的逆境处理间差异显著; 碳酸盐处理的电解质外渗率、POD活性、脯氨酸含量等生理指标变化平缓,与对照差异不明显; 干旱处理和盐与干旱协同处理的各项指标波动很大。相关分析表明: 不论是碳酸盐胁迫还是干旱胁迫,欧李的电解质外渗率、MDA含量和POD活性存在一定程度的正相关,碳酸盐处理下POD活性与电解质外渗率和MDA含量间达到显著的正相关。
Abstract: In this paper, Cerasus humilises in Huangshandian was used to investigate effects of NaHCO\-3 and drought stress. Several physiological during the trgatment with NaHCO\-3, drought as well as NaHCO\-3/drought
combination for 25 days. The results showed that there existed significant difference in chlorophyll a, MDA and proline content among different treatments. No significant physiological variation was found in electrolyte exosmosis percentage,POD activity and proline content between the salt treatment and the control during the treatment while the parameters in drought and salt/drought treatment fluctuated remarkably. The correlation analysis indicated that positive correlations existed between electrolyte exosmosis percentage,MDA content and POD activity in both salt and drought treatments, and significant correlations were also found between electrolyte exosmosis percentage,POD activity and MDA content in NaHCO3 stress.
全 文 :! !"" ! ## d5{}‘7EF;eZ GHEd44/.f4^LL
$ % % &$ # %
& ’ ( )
’()*+,)- ’)./-* ’)+)(-*
/012!"*+02#
3456*
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
$ % % &
*+,Zt’-.u/4&474Uvv"
JKL7M$ NO6# -PQ7 RS6$
972 $J&’c) 0tI 7"%%!%L$2 JKB&’[bKB=4qO JK 7%%$%&L
#2 }tm1’$O)> }tm !8$%%%Q
! "# XEuT-2/4<= $" 9U‘3_ +4:(;# 45T4‘t’T4h3*+,Gt’?hT4*|:-
.#4qf[;4{C"U={*<=/4ughbc-.U67K@g5hijk/4UrrI 4‘8?9Z:
;+;efghU7cT44y)BNL*+,T4U
SVt’-.*/4U
$%&k /4Lt’-.L*+,-.L&4C"
’()*+#’87> ,-./0#- ,12+#7%%7 ? 8!>>3$%%&4%# ? %%7& ? %"
wxy#k$%%> ? %> ? $>g
|}z{k;@&’AEF($z{9$%%@ ? #>Qg
" lQ}s,-./g
!""#$% &" ’()*+# (,- ./&012% 3%/#44 &, 52647&8&17$(8 *2(/($%#/74%7$4
&" !"#$%&% ’&()*)%"%
AB4CD EBCDFGH7*$ (GIC ’GHJBCD7 K4CD LBHMH7 .H N40JBCD$
972 !"#$%&’($ )"#&($#* +,-.(-$* /’#0-, 7"%%!%L $2 1&,’2 345-,-($#’$-", "6 )"#&($ 7&&4( ’,4 7&&42-,8( "6 9&-:-,8 9&-:-,8 7%%%$&L
#2 7’,5&,;-’ <#"6&((-",’2 =&>%,"2"8* ?,($-$@$& 7’,5&,;-’ !8$%%%Q
9:4%/($%k )C OGBF P4PI5*A’(@( %@5-2-(&( BC :H4CDFG4C9B4C Q4F HFI9 O0 BCRIFOBD4OI ISSITOF 0S +4:(;# 4C9 950HDGO FO5IFF 6
’IRI541 PGMFB010DBT41 9H5BCD OGI O5D4OJICO QBOG +4:(;#*950HDGO 4F QI11 4F +4:(;# U950HDGO T0JVBC4OB0C S05 $" 94MF 6 ,GI
5IFH1OF FG0QI9 OG4O OGI5I IWBFOI9 FBDCBSBT4CO 9BSSI5ICTI BC TG1050PGM11 4*3=- 4C9 P501BCI T0COICO 4J0CD 9BSSI5ICO O5I4OJICOF 6 +0
FBDCBSBT4CO PGMFB010DBT41 R45B4OB0C Q4F S0HC9 BC I1ITO501MOI IW0FJ0FBF PI5TICO4DI*<;= 4TOBRBOM 4C9 P501BCI T0COICO VIOQIIC OGI F41O
O5I4OJICO 4C9 OGI T0CO501 9H5BCD OGI O5I4OJICO QGB1I OGI P454JIOI5F BC 950HDGO 4C9 F41OU950HDGO O5I4OJICO S1HTOH4OI9 5IJ45X4V1M 6
,GI T055I14OB0C 4C41MFBF BC9BT4OI9 OG4O P0FBOBRI T055I14OB0CF IWBFOI9 VIOQIIC I1ITO501MOI IW0FJ0FBF PI5TICO4DI*3=- T0COICO 4C9
<;= 4TOBRBOM BC V0OG F41O 4C9 950HDGO O5I4OJICOF*4C9 FBDCBSBT4CO T055I14OB0CF QI5I 41F0 S0HC9 VIOQIIC I1ITO501MOI IW0FJ0FBF
PI5TICO4DI*<;= 4TOBRBOM 4C9 3=- T0COICO BC +4:(;# FO5IFF 6
;#6 <&/-4k A’(@( %@5-2-(&(L950HDGO FO5IFFL+4:(;# FO5IFFLPGMFB010DBT41 TG454TOI5BFOBTF
/49A’(@( %@5-2-( QVW;7AUs[=>
(9Y0F4TI4IQABI9A’(@(Q?+]BhZ*hM@
b*}’‘6}‘}@*RAceRr>SU]u*V
gmo*Ur{A-‘d>]u‘AB{C43Df
P]%rUZ[g$% Ub >% $nh.*W;(<
>./u/4<=2ce<=*?@[dLXTU
Gwp9lAcq*$%%"Q‘&f)749E+?*
$%%@Q‘J^D[GF[CD9lAcq*$%%8Q‘z>
F[GHG|C$O9IJKq*$%%8LL_Mq*
$%%"Q‘hGFdG2>9+A.q*$%%>L4(;q*
$%%"Qqg+u/4674<=Uv->w9{N
q*$%%#LOA?q*$%%>Qg
x<=u/4<=‘3_U*+,T4‘t’
T4h3,Gt’?hT4*=BffP,Zt’
HIQUoR4{C"U={78*QR/4},P
4Z}’4U4{K@*s/4F[sDS:|^g
7 ,EG7K
=>= @AZBa
$%%@$q3 $$&T-2/4G&K9jJK
B&’[bKB=4qOa|Q[|j$J&’c
)&BD[|{TEcUF*:1UVW #% TJ‘‘
!" #$UlV:*QV %tg^HU !&&’$ ’#(%
fcUF<=*,Es )$&G&Kg?#F * [
T4k%Qu] lKBWNPtXZ*h!Q1f4BWNPL!Q,T4 lKBWNPtXZ*
h!Q\ ! +W %% )&& $$,-I./ %U 01234) 45
)&& $.*hiQ1WGeNPL)Qt’T4 lK
BWNPtXZ*h!Q\ ! +W]NP )&& $.L*Q
,Gt’?hT4 lKBWNPtXZ*2!Q
\ ! +W %% )&& $$,-I./ %U 01234) 45 )&& $.g
QRT4 *c1t*)%E$*?T4 !" +*! !51
Z[$PPl-.*Y4BWPtXZgXEfT
49^:U! )*5*6*%!*%"*!&*!"Z )%1u^,X
T4ZKt+rfgF<=4{C"|^*! )%f
\’s$P!\’g
!"# C*n+
|:UC"A
S‘YZIGqrrI;ed-‘8?9989:Q;
e‘[\{f]{ 9^;49QZ9\{f 9^<49Qd4
Z:;+;eg
aKa+rrIGS‘YZILc}dd@K9{
(>q*!&&*Q|::;+;eg
!"$ LDBa
u|*\’U?’-<=7yXeZ^LX
e*mX\’<=_4eFfg.o 8>#?,@,AB CD#E-
DI.Vg
! 5hGXe
#"! EFx;GH7%&’Iu
,‘t’-.u/4
T4U
jBUiaLft’Z,Gt’?h-.HIQ*
Qgh{|}JT4i4%s^sR,^u?:#
BgHl‘!QlUmn*QghiG4jR#U
^uiGB!#UjkUtF=*QgikiU^
_fT4!#Z[2Zg
V % 7c-.u
#!# u] 3,KB?,,-L#"# ,T4 0123,) @B?E@@L### ,Gt’#T4 01234) 1K+ +?,NHJB @B?E@@L#O# t’T4 9?,NHJB @B?E@@I
Qh PJE @1$E 7E-,QI
1fUQghrGjlmZn5d6F*lm
fd4\.fUr"QZ29\{*
m9\{UEGif*89:,h|Qo!*mhG
n5d‘p+_J*d=i/cXbU6T*6Ka
i&^q_J*,orrm+*SmKs^IXb4
Utgeu*6v@n5dU3FgH@*89: U
qPm+uhZQg,Fs:Uia*?h;eU
={mh_0Qghik^_gV % SBT*/4
89:;efGRT4#4i(sku]T4$eg
cL,Gt’?h-.ZKst’-.fT4UR
51c>Ql*Ks*+,-.T4kA|l*! 5
F !& 1;T4’7l‘!lb*! !" 13$P!
={d0?@<’bUu]L,T41tU 89:
;efT4:#‘Uu]‘t’T4Z,Gt’+
hT4*i/.j/4UQghu,T41FwL
&! & ’ ( ) *""
o-.!#*,T4QghU
T4’jBbUu]*i.j/4m+SA0>U
*+,GJ4g
!"! JKLMN
V $BT*,T4Q/4U %&"d4fGRT
4#4={d0?@R<’k‘Uu]L,Gt’
+hT4Zt’T4^7l‘!lbmn*t’T
4UlbmncU,Gt’+hT4*!’bUu]g.jKsU*+,-.‘M_Ut
’-.Z,Gt’+hT46+a@ %&"d4*+
VJl-.i4U%s*d4\3fifv@2[
_^U3F‘2n2^UXP*,o#t %&"d4
Ql*7EVt’T4Z,Gt’?hT4g
V $ 7c-.u %&"G (&"d4Uvv
)*+,$ -../01 2. 13/ 415/44 26 %&" 768 (&" 701*9*1:
V ; 7c-.urrI 73rrI 7;eGrrI <;ed}Uvv
)*+,; -../01 2. 13/ 415/44 26 03=252>3:== 7 0261/61 768 571*2 1?//6 03=252>3:== 7 768 03=252>3:== < 0261/61
V $SBT*;RT4U (&"d4fGRT4
#4={?@*;T44 (&"d4*AjBy)*
$P!;T4 (&"d4’^|lmn*<’‘Uu
]*,Gt’+h-.U|l>__cg/(V.
fgh-.T4#4*(&"gVrGUDFd4Nlfd*o-.Pl!*(&"fNl1fDFd4
\7j{j2rG.og
!"# OPQ7*RSTQ
rrI;eV_01fb3>_UEGC"g
V ;BT*fT4UR ’@ 1*;T4rrI 7={
?@*! ’$ A ’B1;T4’YZ|l*+T44y
)gc*! ’B A $B 1;T44.fjBy)*<’
bUu]*$P!^|lmn*t’-.|lU>_
_c*.jj{|-.urrI 7,FUiam+
XZ3$*j]b,FUia3$@Jg
rrI 7;eGrrI <;ed-mh_0r
rD:SxDUyz^_*Ygh{zP=USx
DdugrrI 7;eGrrI <;ed-2b*
SxDU{z^_2bgSxDhU?:4/2
y*tKb+f $RbMCa4UXvijvv*,
olbb3.o9c/|q*’CCCQgV ;SBT*-
.#4;T4UrrI 7;eGrrI <;ed-
^@JQlmn*f:,Gt’+hT4U={>
_0c*T4U! $@ A $B1q-jBbUu]Z,
6t’KsT4*.j?h-.uSxDU{z,
FUkim+0c*R
V ! 7c-.uS‘YZI‘S‘YZI;eGrrI ";ed}Uvv
#$%&! ’(()*+ ,( +-) .+/).. ,0 *"/,+)0,$1 *,0+)0+ "01 /"+$, 2)+3))0 *"/,+)0,$1 "01 *-4,/,5-644 " *,0+)0+
oKsT4Gu]4*ABNy)g
1frf:S‘YZImh!wls$Zi$
h5Ufv"E$sUb**drrI!wU9o
b+*/VFX6\{#g5lfb3.o:SA
s:m+5*fQgFSmh!w"E+*$%d4
\*,oDfhd9\{97".4)08,/" !" #$ &*9::;Qg
V !BT*GrrI ";e^j*T4R :1;T4
4S‘YZI={?@*;ek‘U6qUu]*.
jM_U-.+ZaS‘YZIU3FL! : < 9=
1;T4YZl‘*,T4l‘U>__c*f%V
t’T4*+hT4l‘>__+La!,T4^
0jBUQlmn*fET4={?@*<’bUu
]*+hT4TU_bP?L$P!t’T4US
‘YZI|l*ofvT4[\Ql6??mn*.
j{|-.uS‘YZI,FUiaXZ3$*o
]biaU3$&G1si4g
S‘YZI;eGrrI;ed}U={VG
1f&i7cU+4AL*/+_0rfb3d4
U>19>$44)8)0. !" #$ &*9::!QgV ! SBT*fG
RT4#4*,Zt’KsT4^(7lb!l‘
{lbUmn*Gu]U={mn^h**AjBy
)L+hT4fT4R 9?1Gu]={^_*h!
^lbUmn*R
!"# UVWIu
V =BT*Gu]^d*;T4U:;+;e^
7l‘!lbUmn*zV|lGlbUi4gh*
+’‘Uu]gR#;T4:;+={d0?@*
;T4a4*AjBy)L:#;T44y)0
c*,Gt’+hT4U={>__c*f%Vt’
T4L$P!;T4’^|lmn*+h-.|l
>__cgT4! @1*,T4‘t’T4Z,Gt
’+hT4U:;+;eXEVu]U 9AB@*BA:C
Z 9A=:5*T4! 9=1XEVu]U 9A@?*=AD@Z
:A!5*T4! ?= 1XEVu]U 9A=C*?A:: Z
?AD:5*.j.ss[{|T’fd*:;+fT
4U:‘!#u^\QghU?:4‘D_dh|4
7j@k2EG.o*7EVfT4:#g
V = 7c-.u:;+;eUvv
#$%&= ’(()*+ ,( +-) .+/).. ,0 5/,4$0) *,0+)0+
!"$ sal.XY%Z$N
i 9BT2gh7c-.Q/43R&4C"
a4U^L4gfP,-.Q*/4U
M\cof BA@ < BACa4*h| ;RC"G IHEd
4a4.fs:^_UL^LL:;+;eG
M*oG IHEd4a4.fBNUL^Lgt’-
.Q*
P,-.^h*h| ;RC"G IHEd4a4.f
s:^_UL^L*V ?/BT-.T4R# GHE
d4W>*oT4!# IHEd4W>*.j GHEd
4G IHEd4SAs:U+h.o*+Vv;{.
?? & ’ ( ) !="
oUi4.fy)L:;+;eG
.fN1UL^LL.jJl-.^_UgH2
$*:;+.ss[{|T’fdf^_Qg{|
n7j@k22.2>U.ogj@mC*/4f
P,T4Zt’T4HIQSA^jU&4_J*
i/,(jPQLP,-.,gh^_{,F1f
U&4t’*H@5;m+SA^jU&4K@g
5 ! [\]^_‘abcsal.XY%Z$N!
"#$%! &’(()*#+,’- ’- .’/) 012.,’*’3,4#* ,-5)6). ’7 !"#$%&% ’&()*)%"% $2 +1) .+()..
T4
&’()*+(,*
&4C"
-./0121304)2 0,5(6
(2(4*’12.*( (61/+1/0/
!"#;e
!"# 41,*(,*
-%"d4
-%" )4*080*.
$%"d4
$%" )4*080*.
rrI );e
9:21’1;:.22 )
41,*(,*
*+,T4
<)=9%>
*’()*+(,*
!"#;e !"# 41,*(,* ?@ABC> >>
-%"d4 -%" )4*080*. ?@DAC DDE! ?@CFF GGF!
$%"d4 $%" )4*080*. H ?@EG A>C H ?@BA> ?F? H ?@GC> AI?
rrI ) ;e 9:21’1;:.22 ) 41,*(,* ?@>BF IF> ?@EDC B>F ?@ADD ?>E ?@GDG GAF
:;+;e -’120,( 41,*(,* ?@AEF GEA ?@C?D B>>! ?@AFG DI> H ?@DEE >E?! ?@?GF EED
t’T4
"1J3:*
*’()*+(,*
!"#;e !"# 41,*(,* A@GCG EB?
-%"d4 -%" )4*080*. ?@BCI ABC ?@>EC D>A
$%"d4 $%" )4*080*. H ?@FIB D>? H ?@GE> GI? H ?@AGA ?D?
rrI ) ;e 9:21’1;:.22 ) 41,*(,* ?@>F> DFA ?@EEA ICB ?@BAG G>? ?@DEF EEB
:;+;e -’120,( 41,*(,* ?@CFI D>C! ?@CFB FEA! ?@G?D C>C H ?@?E> BI? ?@BDF GE>
!!k!"?@?E K
> 5AGBA
ft’G,+hT4Q*/4,9hQlW.
)67ckFQW>[_^d4 *+,‘t’T4
> 5!*;T4U -%"d4jB‘Uu]LGQ2p
S‘YZI3F -.R#;T4US‘YZI;
e‘Uu]L>QW2{|T’fd:;+;e
,T4‘t’T4Z,Gt’?hT4U! FE1:
;+;eXEVu]U F@AG*E@DAZ I@B5g
<=ijKsUP,T4g+u/4UhM
C‘^ MCZrrD,Fia*+VKsUt’T4
+uf,Ft*Uia*mCG}’+4^d/4
SA0>U},+4g+/4f GE 5 U >??
++12ILH F <)=9%> T4QfrrI )ZS‘YZI
;e^jBQlmn*i.j/4SVij2s:
^_UiagA<=ijrrIVSxDh|@I
n5$3DUEGkF*f,-.Q*1frfQg
:rrIGrrDn5453=*e+*K1oU
rrI,jjU*rrI;eQl9~0q*G??EQg
c=x<=ij/4m+SA0>U},P4*+
jUT4i4A<*5V!+J}is#U‘,P
-.SAOM N , -
E+? K G??A K/4U&=74G|C$O K-7&’($*G??A9FQk
D H IK
OA?*T ? K G??C K]uPX-.u/4oKb33rrI-b
74Uvv K&’()<=*GF9>QkGIE H >??K
h{_*. /*u 0*q KG??B K1f&4GH KG% K0tIk0t
I>’c)L%& K
4(;*lAc*l}(*q KG??E K/42>F[G1j2>7o K"
iF2>*G??E9>QkGF H GBK
+A.*{0_*_}#*q K G??C K2&/4&s#k3d4:gh
8=5;eU={3f^L4 K56)v*>E9EQkA>F H A>AK
lAc*Vs.*7@? K G??D K/4J^D[<=
lAc*Vs.*4B$*q K G??E K/4[dLXXY3[QXS7
4<= K-7hZ*G??E9FQk>E H >AK
~ 0*yj‘*4;8*q K G??E K,-.u0[5&r{Z[rf
:rrI;ee=={Uvv K-$"’c))v*>A9FQk>?
H >BK
IJK*z9?*’@d*q KG??D K/4:a;
{ N*X=> K G??> K/4U&f)74G6’K4 KJK"’*
G??>9IQkF? H FFK
{(>*?H@ K G??B K1f&4)GHC#k! >% KJKk‘qa
DL%& K
c/|*A-B*\Cb K FIII KS‘YZINld4\"j|UK
4 K{),v*9BQkF H BK
L_M*P+c KG??E K/4Uk3CZGpnGH$O KF.x"’
c))v*GA9FQkFD H G?K
!)/2(,M18) L &*N),(8 O*-1;18) L -K FII>@ #5);*)*01, *1 /)20,0*. )/
+1,0*1’(5 P. -$QQ 16.3(, (81280,3 ’()4*01,/ 0, P)’2(. *:.2)M105/K -2),*
-:./01213.*FBGkAGI H A>B@
R022(M(,/ =*S),4)+; R*8), !1,*)3J !*"# $% K FIIB@ %T1,(*/J27J’
501605(* ),5 1T1,( J2*’)8012(*UV :)8( /0+02)’ (77(4* 1, +UW<#
)44J+J2)*01, 17 ),*01605),* 3(,(/ 0, <041*0),) ;2J+ P)30,07120) LK -2),*
-:./01213.*F?AkF??D H F?FB@
!"#$% &’()
>G! ># }$~qk*+,Zt’-.u/4&474Uvv