The tender buds of Phyllodoce caerulea were used as explants for this experiment. The most suitable culture media were screened for shoots regeneration directly from bases of the tender buds, rooting and germplasm preservation in vitro with a uniform design. The results showed that DR+TDZ4.00 mg·L-1was the most suitable for shoots regeneration, and the rate of regeneration was more than 98.5%. MS(modified)+IBA0.05 mg·L-1+NAA0.01 mg·L-1+KT0.10 mg·L-1was the most suitable for rooting, and the rate of rooting was more than 97.8%. N-68+B92.30 mg·L-1+ phloridzin 1.50 mg·L-1was the most suitable for preservation in vitro for 42 months. Stems each with one node were cut from the regenerated shoots and cultured for propagation, and a 35-fold proliferation rate was achieved within 40 days. The method of “deferring growth with dwarfing” was utilized for germplasm preservation in vitro at normal temperature. In vitro culture and germplasm preservation in vitro system of Phyllodoce caerulea had been successfully established.
全 文 :! !"" ! ##
$ % % &$ # %
& ’ ( )
’()*+,)- ’)./-* ’)+)(-*
/012!"*+02#
341 5*
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
$ % % &
AnBLkGCDG6?@X(=H!|E
sty6 u v$ w x6 yz{6 .|O6
662 Y*o-)D/0O Y* 67!%%$@$2 )>o-F)/6())D Mu 67%%$!X
$%&t ++&@v,:0Iq@;BwI@.a>
’()*+#’#682!9C:&!726 ,-./0#- ,12+#6%%6 ; #!883$%%&4%# ; %6!% ; %"
PQR#t$%%8 ; %" ; 6&o
/STUt4&C([qG_()/SghTU6$%%#%"(%"X@Y*o-)DG_()/SghTU6<’%#%%#&Xo
!"#$% &’(#")"*$(+ ,-.*/0 "+% 1/’0#2".0 &’/./’3"*$(+ !" #!$%& (4 ’()**&+&,- ,.-%/*-.
=4 >?@A046 .40 BC?$ (D0 <4E6 3?DEF G4EH?DE6 IA4 34EJ?6
662 !"#$%&’"(& )* +,)-)./*0)(.12$ 3)%’$- 4(,5"%6,&/ 0)(.12$ 67!%%$@
$2 7,*" 89,"(9" :)--"."*3)%&1"$6& 3)%’$- 4(,5"%6,&/ :1$(.912( 67%%$!X
56.*’"7*t ,AC HCEKCL M4KN 0O ;1/--)<)9" 9$"%2-"$ PCLC 4NCK DN CQR1DEHN O0L HA?N CQRCL?SCEH 5 ,AC S0NH N4?HDM1C T41H4LC SCK?D
PCLC NTLCCECK O0L NA00HN LCFCECLDH?0E K?LCTH1J OL0S MDNCN 0O HAC HCEKCL M4KN*L00H?EF DEK FCLSR1DNS RLCNCLUDH?0E ,( 5,&%) P?HA D
4E?O0LS KCN?FE5 ,AC LCN41HN NA0PCK HADH >V W ,>I!2%% SFv.; 6 PDN HAC S0NH N4?HDM1C O0L NA00HN LCFCECLDH?0E*DEK HAC LDHC 0O
LCFCECLDH?0E PDN S0LC HADE &82"X 5 Y’6S0K?O?CKXW )Z-%2%" SFv.; 6 W +--%2%6 SFv.; 6 W [,%26% SFv.; 6 PDN HAC S0NH
N4?HDM1C O0L L00H?EF*DEK HAC LDHC 0O L00H?EF PDN S0LC HADE X 5 +\98 W Z&$27% SFv.; 6 W RA10L?K@?E 62"% SFv.; 6PDN HAC
S0NH N4?HDM1C O0L RLCNCLUDH?0E ,( 5,&%) O0L !$ S0EHAN 5 ’HCSN CDTA P?HA 0EC E0KC PCLC T4H OL0S HAC LCFCECLDHCK NA00HN DEK T41H4LCK
O0L RL0RDFDH?0E*DEK D 7"\O01K RL01?OCLDH?0E LDHC PDN DTA?CUCK P?HA?E !% KDJN 5 ,AC SCHA0K 0OVKCOCLL?EF FL0PHA P?HA KPDLO?EFZ
PDN 4H?1?@CK O0L FCLSR1DNS RLCNCLUDH?0E ,( 5,&%) DH E0LSD1 HCSRCLDH4LC 5 =( 5,&%) T41H4LC DEK FCLSR1DNS RLCNCLUDH?0E ,( 5,&%)
NJNHCS 0O ;1/--)<)9" 9$"%2-"$ ADK MCCE N4TTCNNO411J CNHDM1?NACK5
8/- 9(’%.t ;1/--)<)9" 9$"%2-"$@FCLSR1DNS RLCNCLUDH?0E ,( 5,&%)@4E?O0LS KCN?FE@RA10L?K@?E
+ + &6 ;1/--)<)9" 9$"%2-"$X*’ : 2 (
6*L?TDTCDCX++&B+tBUHde*7QA8o
YI#u0oY<7lgUVI#u0<,6!X
6’nIGZ
_*_xTi*5%@<#T7Q>*4(*u_{
#T7t*u0xllFN64)F*$%%"XoT3
/{<{uXxV,{U*uc9F**{i(*n
w]I\x"\/J:$#1tuBo++&bV
Wu0cOt5%Wzop#qr64)*$%%9Xo
3zacHWz+,*$M0{c^1ge*1d<
za$M0{7Q8G_I#cPQ 6 8%% ] $ !%%
S#<{uXEx~!&E?oT3vs|f$
b*GHF++xMrwO%&u"*L%gDO8
IF#6loTX*$I#-:%R/g.#Hv*
b=XR/lkg%u0mndbPxx:0I
q*[K#3#$oHv*u?Qde/pl#-d
4#*gD;BwIz6xzl^o*[#b8;W
deIJoU3H(#3Ivu0#dbPxu%
)*6./0F*$%%8@
’(o
6 DE{lM
626 ccvCDV’( " %U
PQ $ 7"% S#<{uXE1C++&:rvI$
KLU?wd68#(;XAC1S1%on1S1
%}Mj 62% TSva?*D #"X2;3\ 9% N*{
m "X5Di#A3hX6%2"XX7^YZ 6% S?E*
N6w\\ 8AoDN6h4virqwz*/#
By6c/vJKsz\luSubYDo
62$ -t},./0et1V23 [ >Vu/
0de/*Xj $% Fv.; 6*hi: &2" Fv.; 6*R^ "2#*
H46$9 _ $X‘*nj 6 9%% 1Q*nM4# 6$ AvK; 6*
zS-$z%i ,>Ix/Mi )--65y:L45
q4-.z{u $2%% ] !2$% SFv.; 6x %26% ] %27%
!"v#$ %o> &’(#q4B{ )*++ !"v#$ %v[(/
s%&|z*SA*+Z ,*)+ !"v#$ %vz*O?
@*;$ ,-.[?*
#$ %v[S/s6/VKq’*upI++&fdb
Px\#yz[K3*|C_VKq’]z*6/
SA#loXouPz<++&]/SA#,4x
z*O*CD;BwIa6lLW*%23+X*LD
4%+6%+)X;Br6r %X*HIz6|vSub^u
1+omUP &’(x /00q4d4p?bz*O#
VW*HvXL;<;#SAYvde/o
5 ! "!#3!#$4+cXOF$V!
%&’(! "!#3!#$4)&*+,-. &/0 12321. 02.45/
w:
#56578
Ti 9:;<=>8 ?6!"v#$ %X
!% !)
% )*++ +*%+
) )*)- +*%-
1 )*-+ +*)+
, )*@- +*)-
- 1*++ +*1+
A 1*)- +*%+
@ 1*-+ +*%-
3 1*@- +*)+
2 ,*++ +*)-
%+ ,*)+ +*1+
! !%* !) z{u &’(*/00 #,1q4o !% :BC !) :>5
;=B;5B<>:
1DiG%?) rox %?, %+rzXude/*Xj
%- "v#$ %*hi 2*+ "v#$ %*FK -*@*H46)1 L )XM*
nMj4 % +++ 7N*nM4# 3 OvC$ %ozS|Hq
4# /00G/P0x Q00*HvS& R&+*% !"v#$ %6S
AX*5bXTs:L45t1v/Miq4z{I
l$ +*%+ S +*)+ !"v#$ %G+*+- S +*%- !"v#$ %G
+*+% S +*+1 !"v#$ %o
\de.xx5sNVK61%9*=‘#A$~
~o$de/
iN~q ) vN 1 vHvSde/
++/.O*CD;BwIa*HIz6|vA
^u -+*LD 4%+6%+1X;Br6r )X*HvmU /00G
/P0x Q00q4d4p?b/.O#VW*XL;
<5++&qdA/.#de/o
5 $ "!#3!#64+cXOF$V!
%&’($ "!#3!#64)&*+,-. &/0 12321. 02.45/
w:
#56578
T i 9:;<=>8?6!"v#$ %X
!% !) !1
% +*%+ +*+- +*+%
) +*%- +*+A +*+)
1 +*)+ +*+@ +*+1
, +*%+ +*+3 +*+%
- +*%- +*+2 +*+)
A +*)+ +*%+ +*+1
@ +*%+ +*%% +*+%
3 +*%- +*%) +*+)
2 +*)+ +*%1 +*+1
%+ +*%+ +*%- +*+%
!!%*!)*!1 z{u /00*/P0x Q00#,1q4o!%*!) :BC
!1 :>5 ;=B;5B<>:
Px6
-M2/.deoPI}IJ9RM4#x6^o
%*- 2r[9G: [ QUA3u/0de/*Xj
1+ "v#$ %*H4u6)+ L )XM*nMj4 3++ 7N*n4
# 3 OvC$ %*}zS|Hq4#USi P2 x/ML
)v.a>b/sm%Sj#HSJmn:0I
qo5y:L45*P2 q4Il$ %*)+ S )*1+ !"v
#$ %b‘*+Z )*1+ !"v#$ %vHSJ1#SA9:
?(:f*B{ %*)+ !"v#$ %vA6{s&/M6
7o.a>q4Il$ +*-+ S %*-+ !"v#$ %b‘*+
Z %*-+ !"v#$ %vSAsP)S;B1#*B{ +*-
!"v#$ %Iq ,I%[\~j/MoUSi P2 x/
ML)v.a>+Z;Fq4HvS&v*/0+
+&:0A/MOs +*3.[?*4A$ %)I%#
IqZg
>q4#;Wp?x@B/MO*CD;BwIa*
HIz6^u -+e*LD 4%)6%))X;Br6r 1X*H
vmUUSi P2 x.a>#q4d4p?b/M
O#VW*XL;<5v,:0Iq#de/o
5 6 "!$3!$$4+cXOF$V!
%&’(6 "!$3!$
$4)&*+,-. &/0 12321. 02.45/
w:
#56578
Ti 9:;<=>8 ?6!"v#$ %X
!% !)
% %*)+ +*-+
) %*1+ +*A+
1 %*,+ +*@+
, %*-+ +*3+
- %*A+ +*2+
A %*@+ %*++
@ %*3+ %*%+
3 %*2+ %*)+
2 )*++ %*1+
%+ )*%+ %*,+
%% )*)+ %*-+
%) )*1+ +*-+
! !%*!) z{u P2 x.a>,1q4o !% :BC !) :>5
;=B;5B<>:
6$%&’()* +,-&.%XwJo
! =){)2
/"! +0OP};s?pq 1+2g 344<=r>?
@-A},./0et1Vvw :L4b^o
6r 5Xf;BwIwJzlz6\6r 6X45*T?
lg&7o.oT?lgz9 ! #;1q5ut
"! 7 5"/8@$Xq5/J1zb;1Nt# 7 9:"9*
XNuT?lg#NlN*^ Nno ! 7 # ; $!% I
J91*>c‘CIu ! 7 9:"96 ; <"!
B{ /"88 *.v@A ![S/s%&|z*SA*+Z
5"88 *.v@A !vYvO?@6
A@-)oTX*[1*> 1+2 5"88 *.v@A !mnL
>:L6oj^u :8 eXo[S|v8zS 1+2
5"88 *.v@A !# +0de/1*de 5= q*%:/s
6/F1jO"}*^ \dej #8 qjO"}-M
uSA69 !BX@dej 6=q]/SA4-Mj /"8
C*[1*~AS!,a*z*Os 9<"=>[1*$C
Ic‘-.?*~?4%:L ! >QFoTX*+
+&[S/sP|]/SA#;WYvde/ut
+0 D 1+2 5"88 *.v@A !o
5 " #$%3$%!4GH$VHIISX=)!
&’() " #$%3$%!4*+,-./0 123,4+ 5235 67’+ ’+1 /23*75
z6S
1),BE*,%E C(F,
Ti GBCE()- H6*.v@A !X
"! "/
!H>
! /"88 8"!= 6:"=
/ /"/= 8"/8 6="=
: /"=8 8":8 6<"8
5 /"6= 8"!8 <8"8
= :"88 8"!= "=
# :"/= 8"/= <5"=
6 :"=8 8":8 <6"8
< :"6= 8"!8 9:"8
9 5"88 8"/8 9="=
!8 5"/8 8"/= <9"8
! "!*"/ z{u 1+2x 344#,1q4@! ude #8q#z
*O*!8 e:;>o "! B%F "/ B), C(%C,%E)BE&(% (’ 1+2 B%F 344
),-I,CE&J,KL@ ! &- F&’’,),%E&BE&(% )BE, B’E,) #8 FBL- CMKE&JBE&(%*B%F EN,
BJ,)B., (’ E,% O(EEK,-P
/ "/ FB QR OP};s?pqef-<=r>
?@jO1e5Vvw :L4b^o6r =Xf;
BwIwJzlz6\6r 6X45*T?lg&7o
.oT?lgz9 ! #;1q5t"/ 7 8"8=*": 7
8"8!@$Xq5/J1zb;1Nt# 7 96"/*Nn
o ! 7 # ; $!% IJ91*>c‘CIu ! 7 96"/
6 ; 8"<#!X*0 9#"::9> ? 9<"8#!>o[;1q53
L>:L*n[S/s]/#SAMj !"8 C*[1
v*A/MCS#A/?*_\A3~&zS
3S48"8= *.v@A ! D T448"8! *.v@A ! D U18"!8 *.v
@A !#EtQRde/<*de :=q@A/sLV6
/.j*5= q\@A/sP|%9 : ? = I|K.
69 !OX*%#6/Pe.*A<4s !"= C*[1*.
xA#OJG%H;=+*/.Os 96"<>o$C
Ic‘-.?*~?4%:L ! >QFo4(*+
+&;W/.de/utQR6EtXD 3S48"8= *.v
@A ! D T448"8! *.v@A ! D U18"!8 *.v@A !o
5 8 #$%3$%94GH$VHIISX=)!
&’() 8 #$%3$%94*+,-./0 123,4+ 5235 67’+ ’+1 /23*75
z6S
1),BE*,%E C(F,
Ti GBCE()-H6*.v@A !X
"! "/ ":
!H>
! 8"!8 8"89 8"8! 98"8
/ 8"!= 8"!= 8"8: 6<"8
: 8"/8 8"8< 8"8! 9/"8
5 8"!8 8"!: 8"8: = 8"!= 8"86 8"8/ 9:"8
# 8"/8 8"!/ 8"8: <:"=
6 8"!8 8"8# 8"8/ 95"=
< 8"!= 8"!! 8"8! <#"=
9 8"/8 8"8= 8"8/ 9#"=
!8 8"!8 8"!8 8"8! <<"8
! "!*"/*": z{u 344*3S4x T44#,1q4@!ude 5=
q#/.O*!8 e:;>o "!*"/ B%F ": B), C(%C,%E)BE&(% (’ 344*
3S4*B%F T44 ),-I,CE&J,KL@! &- )((E&%. )BE, B’E,) 5= FBL- CMKE&JBE&(%*B%F
EN, BJ,)B., (’ E,% O(EEK,-P
/ ": 0ec!67vmVq8 Tmn8[(/
sP|z*#SA@-)*a_/.v4/l3@
exyz[K3FTi*C_(damnucRM*
n/.#A-Mj /"8 C*[1v*$+zzl+1
.Ldee) / ? :P[a?Ai*A3/4rHx
5|8zS :"=8 *.v@A !V4:6%g{(x1S#2
,1%x-MX#/.de/<*Hvmn(-M2
/.de69 !CXo58 qu !IRM4#*HeRM
6^:;s :=6[1oGHRMA^#RS4<
>@B/Miq46[:MirFXoTX*++&
]/ucRMde/u QR6EtXD 3S48"8= *.v
@A ! D T448"8! *.v@A ! D U18"!8 *.v@A ! D V4:
:"=8 *.v@A !o
/"5 C1gDE n/.AMj /"8 C*v*xd
ee<_9*$m% : *.v@A !y/f7^<\8A
1()#hi*_\u&f /86y/fc/Z#~
k]x-[965 W!Xc5#/,<*DBn-#j3
WMpB[IFIH*F4I\$ 6=>*H4Il
$6!< ; /XX*HqG_nM < N*Hq<^Y&Z
@ !8 *&%*!8q\>8WM*Hq8^57cw6 !
/5! & ’ ( ) 5="
AorwOs !"#[169 $%Xo
&’( )*+"OP};s?pq ,! 65FGV=H<
=r>?@2r[9G:Vvw :L4b^o6r
+Xf;BwIwJzlz6\6r -X45*T?lg
&7o.oVWU#6f71*XxT?lgz9 !
#;1q5ut"$ . &’/*"& . $’(*A3Y&lg*z
b # . $’/$*Nno ! . # 0 $!% IJ91*>c‘C
Iu ! . 1’"(26 0 &’2(34 &X*0 1’"&! ( 5 1’"-" (o[
1*> ,!&’/1 67v84$ 9.a>$’(1 67v84$mnL>
:L*pqrstAHSJ|v8zS ,! &’/1 67v
84$x.a> $’(1 67v84$# )*+"de/1mnI
q*Iq $& I%HSJ1#SA:;/MO1u
1’"/*^ \Iqj 2&I%\*U3:;/MOu
1’"(#[?*$CIc‘-.?*~?4%:L :>
QHoTX*++&HSJ#;W:0Iqde/
ut)*+" 9 ,!&’/1 67v84$ 9.a> $’(1 67v84$oC
DXde/*$+HIJ?*Iqv‘Ms 2&I%[
1*SA#OJG,1F;Ns&)*69 $3Xo
f 2&I%#Iq\*AHSJ5|8[S/s
P|]/SAde/ ;< 9 =;> 2’11 67v84 $1m
nN#Iqde*de 21q\*SA[q5+tD
E5u&t_*^ \dej +(q*Tj2,/M3
J*HSJ1#Sj^\/M}-MuA*YvOs
$11#*~A}SG/MVW*OJx%H;’9:9
+69 $?XobIq\/s]/#SAmn/.:L
%:*de &(q$A/sx(s;6/.j*21q
\.M4s $’1 @6 [1*?’Iq]#/.,4
P*~/.Os !!#[1o
5 ! "#$3#$$4GH$VHIISX=)!
%&’(! "#$3#$$4)*+,-./ 012+3* 4124 56&* &*0 .12)64
z6S
=A3BC63DC @E%3
Ti FB@CEAGH667v84 $X
"$ "&
!H#
$ $’&1 1’!1 $’//
& $’/1 $’21 $’&2
/ $’21 1’+1 $’/1
2 $’(1 $’$1 $’&1
( $’+1 1’(1 $’&(
+ $’-1 1’"1 $’$(
- $’"1 $’/1 $’1+
" $’!1 1’(1 $’$/
! &’11 $’11 $’1/
$1 &’$1 $’(1 1’!&
$$ &’&1 1’-1 $’11
$& &’/1 $’&1 1’!1
!"$*"& z{u ,! x.a>#,1q4@! uIq $&I%#
/MO*0/MM4U/M\"M4#?>*$1 e:;>o "$ BD%
"& BA3 @ED@3DCABCIED E? ,! BD% JKLEAI%MID A3GJ3@CIN3LO@! IG 7AEPID7 ABC3 B?C3A
$& 6EDCKG JA3G3ANBCIED*CKBC IG CK3 ABCIE E? 7AEPCK CE CECBL L3D7CK*BD% CK3
BN3AB73 E? C3D QECCL3GR
5 7 AnB0hXH!s|EWJKLjk)2=)!
%&’(7 813.122+-* &*&692+2 .12)64 -, /+:.-5.-5&3&4+-* &*0 :-*21.;&4+-* !" #!$%& +* ;&.+-)2 24&312 -, ’()**&+&,- ,.-%/*-.
dewx
STLCTA3
GCB73G
T?lg
<37A3GGIED
3UTBCIED
‘0b1
VB6JL3
GIM36&X
jLSO^
WTLCIJL3 @EAA3LBCIED
@E3??I@I3DC6’X
{|zO8
<3GI%TBL GCBD%BA%
%3NIBCIED6(X
OL>
SK3@X
NBLT36)*X
>t>
SAICI@BL NBLT3
/sSAz* VKEECG A373D3ABCIED ! . (2’" 9 !’/1"$ $1 1’!2! + /’&$" -/’2& )61’1$*$*"X. $$’&+
qdA#/.
9 $ ++&dbde2v,:0Iq
FI7R$
3R Z3A6JLBG6 JA3G3ANBCIED +, -+*./@? R ZAEPCK GICTBCIED A3L3BG3% ?AE6 JA3G3ANBCIEDR
< "#{=#
:L>s*de/ ;< 9 =;> 2’11 67v84 $b+
+&[S/sP|]/SA#YvBq;-*++
&dbdeC_6/[S/sP|]/lo*yz
[K3-*,4PGz*O
84 $@<5{++/.*$de/
]/ucPxC_(da*$/.de/#/J1
/2$! -#
#2,1%x-M*x!?QPRM4#*z
]/ucPx#UzoU]*7?Su%s_S{
u0v,Iqlq#)**F_C_BHz6G89
*G>‘GBHp5)nMFla6f<&F*,$$-@
./0123*,$$4@+5678 !" #$ 9*,$$#@:0;<=< !" #$ 9*
,$$)Xo0VWb++v,IqC_VU*L)
/MZ#la6:e2F*,$$-@
?@A>de/<|^S&/Mix-$z%i*[^
S&USi BC,"!$ %&v’( )x.a> )"#$ %&v’( )b
m%e1Sj#HSJmn:0Iq*=vla4
$+H?Iq++&v,s -,I%[1oYZb
N#Iq#HSJmndex/.:L>stN#
Iq\HSJsPTj/M*Sj2sP/M-M
rA~/MVW*OJx%H;’9:9+*A#/
.,4x/.Ot@Iq]P!
#o0VWpq>stur1c]P++&
bM0{c++L%gDGzu*HAxv,
:0Iq%XK#$mqro
s t , -
:e2*c=g 9 ,$$-" Txj~s:0A)f/MaIq23yz
[K3VW 9 FG))*!)6-Xt--) ( --A"
lLW 9 )C>$" ;BwI2^7la$:LwI#gD 9 gD^)
))*!6-Xt!A! ( !4,"
./0*G*q*" Qq’:#q’deUP,x
M 9 u0/6)Yk*--6)Xt)!!"09 La’:#q’deUP,x
M 9 u0/6)Yk*--6,Xt!$$"D9 _e\#q’deUP,xM 9
u0/6)Yk*--6,Xt-!,"59 eYg;d#q’dexP,
xM 9 u0/6)Yk*--6)Xt)!A"79 La’:#qdPx2v,:
0Iq[9 9 FG))*!#6-XtA$! ( A$A"M 9 u0/6)Yk*-!6#Xt>C>"
f<&*"d)*N+_*F 9,$$-" )l{5O.8;D8==6~:0rIxB
Hz6#*5jk 9 FG))*!)6AXt>,>"
n +*N < 9 ,$$-" <70vR_<,t!X" 9 >?t
4 )*"ur*{u& 9 ,$$#" <7M0{T7u0P_9Y 9 4
&t4&$H9^_*->4 ( ->>"
4 ) 9 ,$$A" M0{c’:2(g%UVu0#cOkYxI#
PQ 9N>F)))*,>6-Xt!C! ( -$A"
+5678 E F*+;;078G0 :9 ,$$#" H= I
5
./0123 B9 ,$$4" H= I
6!Xt-$- ( -$4"
!"#$% a b)
--) & ’ ( ) -#"