免费文献传递   相关文献

Analysis,Synthesis and Biological Activities of Sex Attractant forHolcocerus artemisiae (Lepidoptera: Cossidae)

沙蒿木蠹蛾性诱剂的分析合成与生物活性


采用毛细管气相色谱(GC)和触角电位测定(EAG)技术分析沙蒿木蠹蛾雌蛾性信息素腺体提取物。结果表明:在提取物中存在有顺-5-十二碳烯乙酸酯 (Z5-12∶ Ac)、顺-5-十四碳烯乙酸酯 (Z5-14∶Ac)和顺-5-十二碳烯-1-醇 (Z5-12∶OH) 3种主要成分。通过人工合成得到相应的标准化合物,林间诱蛾试验结果显示: Z5-12∶Ac和Z5-14∶Ac 2种组分的组合具有很高的诱蛾活性,且具有专一性,当加入反-3-顺-5-十二碳二烯乙酸酯 (E3,Z5-12∶Ac) 时诱蛾活性增加。将3种化合物按10∶6∶0.5配成性诱剂制成诱芯(825 μg·个-1),诱蛾效果最佳, 日平均诱蛾7.2头。此性诱剂可用以田间诱捕和虫情监测,为沙蒿木蠹蛾的综合防治提供一条新的方法和技术。

The female sex pheromone gland extract of Holcocerus Artemisiae was separated and indentified by gas spectrometry (GC) and electroantennograms (EAG). The result indicated that (Z)-5-dodecenyl acetate (Z5-12∶ Ac), (Z)-5-tetradecenyl acetate (Z5-14∶ Ac) and (Z)-5-dodecen-1-ol (Z5-12∶ OH) the major pheromone components. These chemicals were artificially synthesized. Results of field trials showed that the mixture of Z5-12∶ Ac and Z5-14∶ Ac had high biological activity and specificity. The addition of (E, Z)-3,5-dodecadicen-1-yl acetate (E3,Z5-12∶ Ac) to the mixture increased attractive. The traps baited with rubber septa impregnated with Z5-12∶ Ac, Z5-14∶ Ac and E3, Z5-14∶ Ac in a 10∶6∶0.5 ratio were the most effective. On an average, 7.2 male moths were caught by a trap baited with 825 μg of the sex attractant per day. The sex attractant can be used for trapping the male and for the insect survey, which would provides a new method and technique for the integrated control of H. artemisiae.


全 文 :! !"" ! ##
$ % % #$ # %
& ’ ( )
&’()*+(, &(-.,) &(*(’,)
./01!"**/1#
&234*
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
$ % % #
F6,pS0Gn:?@N2LH7B0
7gk5 lZ_$ :Dm$ qn56
A51 -Yz’[)JI)F qu %6%7%5X$1 /W&’[)&)F /W 5%%%76X61 cdA6tG&+,;*ls A6 8"%%%5c
! "# KP))3zSA8A9’cx\l?%$0A),9clB?@F6,pSqS0Zl6WOJ’7lmn
opqdJ’7BzdZ8 : " :nj1rrGsA!" : 5$; ,nj1r : 5 :pA!" : 5$;=>c641%2?l~@34N2[ZSf:W\}N7*&!" : 5$;,rGsA"6*!" : 5$;,: 5c*GS=
nzE*ftuGS 81$cll0Gn^PY%xlBl
$%&q F6,pSX0GnX})N2XH7B0
’()*+#&8?6 ,-./0#, ,12+#5%%5 : 8!773$%%#4%# : %5%? : %"
def#q$%%# : %5 : 5"l
ghijqCP(lrs_tuvA$%%?A,B%7,5%cl
!"#$%&’&*(%")*+&’& #", -’.$./’0#$ !0)’1’)’+& .2 (+3 !))4#0)#") 2.4
!"#$"$%&’( )&*%+,(,)%A5+6’,.6)+4#q7.&&’,#+c
CDEF@ GHFI/F@5 -J/ K/JLHF@$ C/F@ &DHMHEF@$ -H CDEFN2F6
A5 4 #$%%&’& $( )*+ ,-. /01&-0&*/2,-31 )’*104%+4*,% 5-16&*71+8 9,1’4 %6%7%5X$1 :$*&7+ #$%%&’&*;&1<1-’ :$*&7+*8 5-16&*71+8 ;&1<1-’ 5%%%76X
61 =1-’>4 /+,+1$- $( :$*&7+ ?17&,7&7 ,-. @&7+7 #$-+*$% $( A1-’31, =1-’>4 8"%%%5c
!8&)4#0)q +D2 O2PE02 Q2M 3D2R/P/F2 @0EFS 2MIREA9’cEFS 202I2IRES2<2FU0 E<2IEI2A!" : 5! ; ,cID2 PEV/R 3D2R/P/F2 TH/0/@HPHMIJR2 HF; ,< HF E 5% ;? ;%1" REIH/ N2R2 ID2 P/QI 2OO2ID2 Q2M EIIREE F2N P2ID/S EFS I29+% :.4,&q B$%0$0&*47 ,*+&C171,&XQ2M EIIREF6,pSAB$%0$0&*47 ,*+&C171,&cC|jj
A-23HS/3I2REc,pS(A’/QQHSE2cHl,pSC
A B$%0$0&*47cl1%Ye,A;F6A )*+&C171,
,*&-,*1,cxuvwA@&’,-4C 2,*C,%,c:!?*5‘!
?Ze,-c+ 5%IcAOd)*5#7#c*!?O#
z;*V2?‘6I*\3$H/F"78’W[l
o$,*>,dEC>}~*cd*4Y2j:F6
?k[[3E5H*V2F6+P2q6I*[[}
sBF6dPD*wPDxT0Wjv:q)*wq
)x/0Fj8:/F"P*XECY?:HIJK
TfV2B8[:#>l)k2Pz\8xxz+!2ac*|^BF6,pSe,dF6!?A
;*B)Ry*L4*cZ2AYz=*de8F6[
3HydFDjv*L434*l"’4r{cm
e**l=n%!Idl5l*DEA=*{y*{Y
h*lFt,pS:qac*qlBrZR%AMDEs,0Zl6Y0Gn*l;,$oh$,
587,:o4*l;,:qlBl
^B,pSH:]#yA5 Z !$c*5H1WI
2W5*‘c\,pS0Zl6:DE@AW[l
dC>?*N $%12 8%$W+s*ZT|jjSr
s,0Zl6:DE-’[R%m8*|d,pS
(s,0Zl6:DEprB*dC?*~Zwo,
pSA#$7747 0$7747c2 6 4,pS0Zl6#$0
A!""#$%&’ !" #$ (*)*+,-X)*+,.X/-00$11$ !" #$ (*)*23X
4"5$5$ !" #$ (*)*2,cldC>-5g: ,634,p
SB^&Z}~,pSA%&’’(’ )&*+&$,-(’cA}H/
2*)**6c*{Hl,pSA. ( ,*’($#/,’cA78-59 !"
#$ (*:66)cxFt,pSA. ( 0,11&10#!-&$(’cA;-59
!" #$ (*:66l6N{ST*plwl~#N8B\F6,pS
0Zl6:o,YDE*\0Zl6mnB&??
@*\l?%$0*34N2Y&FD[r&<5
U*-]0B\F6,pSZW!6GB0:0G
n@|xU9*wsRAP0Zl6*lFt,p
Sp+p1g=l
) KKLac
!"! S#{0|H
Nb?F6!>K[3{>*qS=}XL-rZP7nwl~@b
?GS5UxV>.p5g95|A=-##$59cAxw
)*q36s:)q66l9??Y.pxd?|yJ’7
GSB05U‘pqS0Zl6?}Wdd?2 > *
&&<4’ld~#5|‘*0!’"qS*d^j
Y+*P+k>d?*P#_^’0Zl6WOxe
wr*-e :mR!bo$BlJ’EP ?: {*Q
:+mXOE*ze @ )6 AcYBjPl
!"# +^+U] 3^$%4)@
KP B9$#’5% ,2*6zSA8‘*%Uh)_}r
$rA;C!c*?: w6z*{?qmi*))3Cw DE
@ )A<6 F G 6H:: FF G 6H33!F E-#"-#%" /-#$I"J5$-c*U
/wFwq7_FV 26 A*M- : F$5*YK AyF$5@ )
:wFo(*wF+ )26 A*dY6 AyF$5@ ):wFo
(*wF+ :K6 AM- )K F$5lr$rxmirFV
w :<6 Al
!"& EF1("#
0WJ’7&N2W\ZN7:\l?%?@
ac,"Ohd2A:666c*R4VW7dwo!l8tn$0 :m*R(:-S-w ): &*\l-
:K*’tn$0:tuvl
!"’ SGVKFC
!‘?@mnx&;f[:WKxB73fN2wHN2B\V>H$x%cP:W\}N7q
-q’ 75X.q{Fg) E83EMF /,D,X=qbug( * @ NOP$*j
og&) !QRSX&qno* /D3A/D:c*/DSX’q+* /D3
A/D:c(qrjp DS/D:/D:SD*DT M j,o$ /D:/#:X)q: @Ar- @ )cAga%./
NJ/D:/D: @ 4DEMDTX*q%}0( P$B#DK*jrjpo1A/D3S/D:/D:c:SX+q26U@2
rG 26UDSB=X,qbug( 5 @ NOP$*jog&) !QRS* V$E83ETA/D:c,/D3%NJX
-qop /D3SD*\ogF3G /+D2S3RX.qr4, /D3/S/#*.5 /+6)! *# Ohd2qF6,pS0Gn:?@N2LH7B0
! "# SGVGHKInkyPGJ"#
AP8n+Fi"w0Gn6O*SN20G
n&rg7kBCg1Bn2kE*P/fr09
Sbo$kE/e+FiABC()F)7DEO
IV6J\c:(’*kn65R-2Gt*%{s
FMzlP&/eCg1kn:+Fi"\blP
gK78‘n{8C;G0rAd ! "q93 !# $%
& !# $% & ’! $%*8y ’( $%c*So6Z_,F:|
D‘Ad ! )q’! $% & ’( $%cIeG0r>c9%
Bd ! F6,pSG0r1A
*+,- ! ./01$2 34 1567 34 ! - "#$%#&’(’"%
&89 : 8;s89 : !9c*FD[rF6HyjvmnlRh
’Iw < hR(*G0rAP,rR/0*AV
!=8 > !=’ %*G0r~<:()w (8 > !88 %lB
’88?$ 89 : ’8s8? : 8@*dmosU‘GSB0:
w‘*SrZGSB0: )( : !’ A "$*)( : !@ A "$*
*;*)( : !’A "$ ;42?t !8 A< A8=(|e?In2
UZ !<(*;;8*?’(*! ;’8*! <(8!,!wn9:Gt
AOhd2*’88!c*mnBn9\GSB078:
5Ul
!$% <=&G
KP .B.. !;=8 b_=>\-‘mn?@’
I*VP CDE$6Er56 + rUr>s20l
’ mnL?@
& "! ’( k )*’)@\h
< FG?@*F6,pS0Zl6WOJ’7
Ug @hxAd ! "*#*$*%c*M-‘Iwq"A!(=?!;c*#A!#=9’@c*$A’(=’9;cx%
A’9=((#c*9Bx"*#*$:M-‘M-‘W
7Anop G!!I’; : HIc09$0mnopq;h&
?:U9xS\|eu1‘I+:’"qS0Zl6B )( : !’ A HI*)( : !’ A
"$x )( : !@A "$:U9?IwqA9=!@ J 8=9;cE,*
A(@=’8 J 8=;@cE, xA;?=98 J 8=@! A!8 A9cl
d ’ qS0Zl6WOJ’7:zSA8d
*+,- ’ F6K $253%613,56% 34 40%6L0 K0M 72053%3E0 ,L6EN 0M156$1K
-S\l\8@0Zl6WOJ’7&N2r
g7: O"F3f^YZUAd ;c*-S\ )( : !’ A
"$eHz!: O"F3fA(=’! %Pc*L9d7VS
|*r>s2A, Q 8=8(cX\ )( : !@ A "$3fmB
A;=!; %Pc*M\ )( : !’A HI3fW{A8=(’ %Pcl
\ *;*)( : !’ A "$x )# : !@ A "$^eHW!:
O"F3fA?Iw ;=(’x ;=’( %Pc*23X ’47
Vd0Zl6J’7BM#5g*|d%<5UB
5g *;*)( : !’ A "$Zps:8="Pl;O,
Z*) "}N7: O"Fv[B * ": O"Fv*r>
s2A, Q 8=8(clsr}N7 O"FvG[Bpr:
O"Fv*r>%s2A, Q 8=8!clmos‘T0Z
l6BnjAYnac1rprGs^M:<=/L
$8"m/l
&$& bcI)cDI)1yKKPGLfh
No !^Vqro&?{GSB0XtzSA
8]0:0Zl62?|e@n:3&?6GnG
S=ns2*9B R$9{!0Zl6&?:GS=
n]sB R$< j!&?*tp )( : !’ A HIYZ8
="P*Q^!: *;*)( : !’ A "$ x )# : !@ A "$ ee R$< &
?*o#cGSB08!*ohG0rfzAGS9
^) !8 clMR#\GSB0<={"PlXo
mn‘T )( : !’A "$*)( : !@A "$x *;*)( : !’A
"$ ;&?@n:Gt\F6,pS-S:GSB
0z!lw‘^o ’^YZU*SX ; 4&?t!
w|eZ@‘*"A)w !8 A9AwWOJ’7B ’4&
?8! & ’ ( ) @("
!q!! " #$% &’*"! " #$% &’*!! " #$% ()*"! " #$% ()X"q!* " #$% &’*"* " #$% &’*!* " #$% ()*"* " #$% ()X#q!+ " #$% &’*"+ " #$
% &’*!+ " #$% ()*"+ " #$% ()X$q!, " #$% &’*", " #$% &’*!, " #$% ()*", " #$% ()X%q"!*!* " #-% &’*"!*!* " #-% ()X&q!* "
#-% &’*"* " #-% &’*.* " #-% ()*"* " #-% ()X’q!, " #-% &’*", " #-% &’*!, " #-% ()*", " #-% ()X/0123)1A-4/c5
tuv 6W\r*qSR( #-clyz!wo1r>s2A# rU*$ 7 898*cl:;3< 6 =>*% ? #-9 >@AA;2;<1 B;11;2C @A# 1;C1*$ 7 898*c5
d ! F6,pS-S\W\}N7&qS0Zl6J’7A-4/c:\l?%A/(Fc
4@E5 ! /(F 2;CGH9 ! MNhOJS#{0c;*PCKyP"#!
"#$%! &#’( #))*#+),-. -/ ! % "#$%&’(’"% 01,.2 )3( 1(4 53(*-6-.( #.7 #.#’-20(1 ,. /,(’7 A6 L@’I
N2;31I;<1
Gt&?
OHIGHC@1@H< HA P3@1CQ(E
( R O > /
GS;9
NH13B I3B;C )3JEK1
ftuGS9
>3@BS I;3<
123G )3G1J2;C
fzAGS9
>3@BS IHC1
123G )3G1J2;C
N)#
N)-
N)!
N)$
N)*
N)T
N)+
N)U
N),
OVA’;03<;c
*88
8
8
8
8
*88
*88
*88
*88
8
8
*88
8
8
8
!*8
!*8
!*8
!*8
8
8
8
*88
8
8
8
*8
8
8
8
8
8
8
*88
8
8
8
*8
8
8
8
8
8
8
*88
8
8
8
*8
8
8
8
8
8
8
$,8
$$,
*!#
$TU
8
8988 6 8988;
8988 6 8988;
8988 6 8988;
8988 6 8988;
8988 6 8988;
*9U! 6 89-UP
*9!* 6 89#*)
T9!- 6 89$#3
*9T, 6 89!UP
8988 6 8988;
8
8
8
8
8
U
+
#8
U
8
)(q!* " #-% ()XRq!* " #$% ()XOq!* " #-% &’X>q"!*!* " #-% ()X"q!, " #$% ()lOVqCg1 W;12HB;JI ;1K;2 5 +w NK; C3I; P;BHM5
oB-vwRh80rGS9:tu- 6W\r*yz!wo1r>s2A# rU*$ 7 898*c*+wlNK; D313 @< 1K; 13PB; 32; I;3< 6 => HA
9 8 bc1yS#{0c;*PCGHKKP"#
"#$%8 &#’( #))*#+),-. $9 ’0*(1 :,)3 )3( 7,//(*(.)
*#),-. -/ 1(4 53(*-6-.( #.7 #.#’-20(1 ,. /,(’7
A6 L@’ I
N2;31I;<1
Zl6&?|e
X31@H HA C;0 GK;2HIH<;C
( R >
GS;9
NH13B I3B;C )3JEK1
ftuGS9
>3@BS 123G
)3G1J2;C I;3<
N2 #
N2 -
N2 !
N2 $
N2 *
N2 T
N2 +
N2 U
#8
#8
#8
#8
#8
#8
#8
#8
U
+
T
*
$
+
T
*
#
#
#
#
#
89*
89*
89*
!!T
$,T
*+,
--!
U*
*#8
T8U
-!!
$9#, 6 89-+ )
*9,8 6 89*T P
T9U, 6 89$* 3
-9T* 6 89-U D
#98# 6 89## ;
T98+ 6 89*- P
+9-$ 6 89!! 3
-9++ 6 89-8 D
?:|eSwcx #8 % T‘:GS=ngbSw*R
#8e*&X (%R%>w #8 %T %89*‘GS=nzEl
8;< VEVGJmSKZ[
&U9\GSB0Zps:78*GtB0Gn:U
9CA*GS9C3Ao !cln9w# T*8(E:tu
GS9zA*$#T8(E: U9++ v*$!!8(EGt:
$9Uv*G09r>s2l23# T*8(Ex# !88(E
|U-*(E:GtGS9[*|r>!s2l^l^
:*PB,)$*xG;*l2,:Gtn9d
U-*(E|{-^*Xi-<)ZG;x$*:=n
+^&/0Zl6l
! mJL[J
bDE~AP))3zSA8AFOcx\l?
%$0A/(FclB?@*_(F6,pS0Zl6
,8#! ,# Ohd2qF6,pS0Gn:?@N2LH7B0
9 ! QUGLUbcVEKSGVKXYGJE
"#$%! &’($)*+ ,- (#.)+ /#’012 $3 4#*3560
#(,’62+ ,- #22*#/2#62 A6 !"#$%&*’(()
’ I
*+,-./,#.
Zl6&?n9
012-$, 13 2,4 56,+1/1#,27!$
8 9 0
GS;9
*1.-: #&/;,+ 13
/-:,2 <-&$6.
ftuGS9
0-":= /,-#
.+-5 <-5.&+,2
*> ?
*> ’
*> @
*> A
*> B
?((
’((
B((
)((
? (((
C(
?’(
@((
A)(
C((
B
?(
’B
’(
B(
D@
?’@
BB@
B)’
BED
(F)D G (F?’<
?FAC G (F?);
CFB) G (FCE-
CFE@ G (FBD-
CFEE G (FAB-
^<$3!&?O,*d @h&?A!B H ?A I 8<*!B
H ?AI 8!B H ?AI 8< ’&?ogUW!:GSB0lX45
Umnw,pS(: "#$$%$ C:wo,pS
AL-55"MM" &’ () N*?E)@cx}~,pSA}H/2*
?EE(c:0Zl6GS5UmnaOSgl|Lw
CA*#)+#+&,%$c:{,pS0Zl6A1%2?$ !@
H ?AI 8H ?AI 8r>0o4Ogl|ArBU*b5U&$!‘F
6,pS0WOJ’7d))3zSA8AOLcd
B1%2?:M-‘0*9s?@]0w @42?:*NZjBAP))
3zSA8 H\l?%$0$PAOLPQ80cx))
3zSA8 HV8$PAOLPRSc2lBmos?@
$0l
F6?k[5U‘pY !B H ?’ I 8<*!B H ?A I
8<*-@*!B H ?’I 8< @4}N7t ?( I C I (FB@20
Gnn2GtA)’B!$yh
H ?c*GS=nzE*ft
uGS DF’cll0Gn^PY,)7$x%0*wF6,pS:jN*lJ\opq:acx
lBljodEC?k:,pSZ C( 34*9B
*#)+#+&,%$ C:,pSLZ ?( 34*F6,pS0
Zl6:2??@SZ%B-mEC *#)+#+&,%$ C
,pS0Zl6:m/$0VwPs,0Gn*l
F6,pSLVBp>l
;~*l0Gn:28N2L5gw58A=*
{y*{Yh*{y;7$x*lF6,pSA;:
qac*qlBJ\BKKl9fP;vdBZA
BMN^v*(zw;*JA*lF6,pS:=
n*c^YNIcP})z\V2:Yh*MNJ
K*HI=zxwx=zs2l
d e , -
Od) N ?E)E N 4Y,pS?k&F6,pSH7)DE N 4Y&
’(l*AAcqD? H D@N
}H/*S0Zl6DE N &’(
)*’CAAcq@?C H @’?N
Ohd*IV6 N ’(((F {,pS-S\qS0Zl6J’7&N
2W\}N7:?HI3f N &’()*@CABcq?’@ H ?’C N
Ohd*IV6 N ’((?F {,pS0Gn:N2&&’()*@DAAcqD? H DAN
L-5"MM" 8* *1#"#" L* O&$:",:/,.." O* &’ () N ?E)@ N S,4 56,+1/1#,
<1/51#,#.2 13 .6, Q&+15,-# $1-. /1.6*"#$$%$ +#$$%$ N T L6,/ Q<1:*Eq
?E? H ’((N
011:"..:, U Q**-$,2.-> 8*R<#"$6. R QN ?EDC-N *+-55"#$ <-+5,#.,+%1+/2
-#> 825,# <-+5,#.,+%1+/2 %".6 2,4 -..+-<.-#.2 "# V1+.6 0-W1.-N Q#X"+1#
Q#.1/1:*Bq’BC H ’CEN
011:"..:, U Q* U1,:132 Y* S1:1/1# T 0N ?EDC;N A !* -cP@*BP
.,.+->,<->",#P?P1: -<,.-., 2,4 -..+-<.-#. 31+ <-+5,#.,+%1+/ /1.6*
.,/#0#12$’%$ ,#3/0/(&AZ,15.,+-*L122">-,cN T L6,/ Q<1:*
’*@EE H A?( N
[-#$ \ !*S&# T K*]6-1 L K*&’ () N ’((BF S,4 56,+1/1#, <1/51#,#.2 13
.6, 2-#>.61+# <-+55,#.,+%1+/* *#)+#+&,%$ 4/55#54(&+#)%$ N T L6,/
Q<1:*@?A?cq@E H ADN
*1#"#" L*L-22-#" O*R-22-+>1 Z* &’ () N ?E)CF S.&>= 13 3,/-:, 2,4
56,+1/1#, 13 :,15-+> /1.6* !&%6&,( 52,/0( !N "21:-."1# -#>
">,#."3"<-."1#13 .6+,, <1/51#,#.2N T L6,/ Q<1:*?’ACcq?BAB H ?C?BN
]6-#$ T **K-# \*R,#$ ^ ]*’((?F S,4 56,+1/1#, 13 .6, <-+5,#.,+%1+/*
*#)+#+&,%$ /0$%)(,/$A!,5">15.,+-qL122">-,cN ] V-.&+31+2<6*BCA’@ H A’EN
!"#$% ./0,
(?? & ’ ( ) AB"