利用毛细管气相色谱分析榆木蠹蛾处女雌蛾性腺体提取物、化学合成相应的标准化合物并通过触角电位分析及林间诱蛾试验,旨在找到一种对榆木蠹蛾成虫具有强引诱活性的性诱剂。结果表明: 提取物中存在反-3-十四碳烯醇(E3-14: OH)、顺-3-十四碳烯醇(Z3-14: OH)、反-3-十四碳烯醇乙酸酯(E3-14: Ac)、顺-7-十四碳烯醇乙酸酯(Z7-14: Ac)和顺-3-反-5-十四碳烯醇乙酸酯(Z3E5-14: Ac)。榆木蠹蛾雄蛾对Z7-14: Ac产生最强的触角电位(EAG)反应,为4.95 mV,极显著高于其他化合物,其他依次是E3-14: Ac、Z3E5-14: Ac、E3E5-14: Ac 、Z3-14: Ac、腺体提取物、E7-14: Ac,其中乙酸酯化合物EAG值极显著高于其相应的醇(P<0.01)。林间诱蛾活性试验表明: Z7-14: Ac有诱蛾活性,E3-14: Ac和Z3E5-14: Ac有显著的增效作用,Z7-14: Ac、E3-14: Ac和Z3E5-14: Ac按10: 4: 4的比例配成每个含900 μg性诱剂的诱芯具有很好的诱蛾活性,单诱芯日平均诱蛾数达11.02头。
The sex pheromone gland extracts from virgin females of the carpenterworm moth, Holcocerus vicarius were separated by gas chromatography (GC). The corresponding synthetic compounds were studied by electroantennogram (EAG) and trapping test in field to screen a kind of sex attractant with strong attractive activity. The results showed that sex pheromone gland extracts contain (E)-3-tetradecen-1-ol (E3-14: OH), (Z) -3-tetradecen-1-ol (Z3-14: OH), (E) -3-tetradecenyl acetate (E3-14: Ac), (Z) -7-tetradecenyl acetate (Z7-14: Ac) and (Z, E) -3,5-tetradecenyl acetate (Z3E5-14: Ac). The Z7-14: Ac elicited the largest EAG response to a male (4.95 mV), followed by E3-14: Ac, Z3E5-14: Ac, E3E5-14: Ac, Z3-14: Ac, sex pheromone gland extracts and E7-14: Ac. The Z7-14: Ac was significantly (P<0.01) different from other synthetic compounds. The EAG values of these synthetic acetate compounds were significantly (P<0.01) different from corresponding alcohols. In field trials, the Z7-14: Ac had attractive activity. The E3-14: Ac and Z3E5-14: Ac could highly enhance the attractive activity when they were added to Z7-14: Ac. Traps baited with a combination of these three components Z7-14: Ac, E3-14: Ac and Z3E5-14: Ac in a 10: 4: 4 ratio at 900 μg per trap had a strong attractive activity. On average, a trap could catch 11.02 male moths per day.