以喇叭沟门和怀柔汤河口2个种源的欧李盆栽苗木为研究对象,测定自然干旱胁迫期间和复水后的各种生理指标变化。结果表明:随干旱胁迫时间延长,2个种源的叶绿素含量、类胡萝卜素含量、MDA含量和SOD活性等指标均发生了明显变化,但除了怀柔汤河口种源的叶绿素含量和喇叭沟门种源的POD活性与对照存在极显著差异外(P<0.01),其他指标与对照均无显著差异(α=0.05); 喇叭沟门种源的电解质外渗率和MDA在处理的第32天出现一个峰值,而怀柔汤河口种源电解质外渗率在处理前期缓慢上升,然后稳定一段时间后下降,MDA含量在处理的第44天出现峰值;不论是叶绿素还是类胡萝卜素含量,喇叭沟门种源都是在前14天急剧上升,然后下降到起始点,而怀柔汤河口种源在处理前期迅速上升,然后在高位浮动,直至处理结束;2个种源的POD活性均随着胁迫时间的延长逐渐上升,胁迫末期达到最大值,但怀柔汤河口种源的POD整体活性水平远远高于喇叭沟门种源; 2个种源SOD活性的最大值均在0.8 U·mg-1min-1 左右,但喇叭沟门种源出现在胁迫的第32天,而怀柔汤河口种源出现在胁迫的第14天,然后下降直到胁迫结束。与喇叭沟门种源相比,怀柔汤河口种源面对短时间的水分胁迫可能具有更强的耐旱性。
In this paper, the seedlings from two provenances, Labagoumen and Huairoutanghekou of Cerasus humilises, were used to investigate their responses to drought stress. Several physiological characteristics were measured during the 50 days drought stress. The results showed that the chlorophyll, carotenoid and MDA content, POD and SOD activity of two provenances all changed during the stress. There were no significant difference between the stress treatment and the control in most of the parameters except for chlorophyll content for Huairoutanghekou provenace and POD activity for Labagoumen provenance. The electrolyte exosmosis percentage and MDA content of Labagoumen provenance increased to a peak in the 32 days, whereas the electrolyte exosmosis percentage of Huairoutanghekou provenance increased gradually in the early stage of the treatment, and then declined after a relative steady stage. MDA content of Huairoutanghekou provenance rose to a peak in the 44 days. The chlorophyll and carotenoid contents of the two provenances increased sharply in the early 14 days. And then the pigments of Labagoumen provenance decreased to the origin level, while the pigment contents for Huairoutanghekou provenance fluctuated in the higher level to the end. POD activity gradually increased, and reached highest value in the end of the treatment for both provenances, however the POD activity level of Huairoutanghekou provenance was greatly higher than that of Labagoumen provenace. The highest value of SOD activity of 2 provenances was about 0.8 U·mg-1min-1, but the peak appeared in the 32 days for Labagoumen provenance and in the 14 days for Huairoutanghekou provenance, and then all decreased until the end of the experiment. Therefore, Huairoutanghekou provenance seemed to have stronger drought resistance than Labagoumen provenance.
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