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Effects of Spraying Microelement on Anthocyanin and the Relevant Biosynthesis Enzymes in Prunus persica f. atropurpurea Leaves

微量元素喷施对紫叶桃叶片花色苷含量及其合成酶活性的影响


以3年生紫叶桃为试材,研究分别喷施微量元素Fe,Mn,Zn,B对其叶片花色苷含量及合成酶活性的影响。结果表明: 喷施微量元素初期,各个处理之间叶片花色苷的含量差异不显著。20天后,喷施Mn,Zn的叶片花色苷的含量增加幅度高于其他处理,此结果证明Mn与花色苷含量的正相关关系。喷施Fe处理对紫叶桃叶片中花色苷合成有抑制作用; 喷施不同微量元素处理对花色苷合成过程中几个酶的活性产生不同的影响,在苯丙氨酸解氨酶、查耳酮异构酶、二氢黄酮醇还原酶(DFR)和尿苷二磷酸-葡萄糖-类黄酮糖基转移酶4个酶中,DFR酶活性变化最为显著,Mn,Zn处理后DFR酶的活性显著高于对照,且DFR酶活性的差异出现在花色苷含量的差异之前,证明DFR对花色苷合成有启动作用。

The variation of anthocyanin and the relevant biosynthesis enzymes in Prunus persica f. atropurpurea leaves was investigated after spraying the leaves with microelement (Fe, Mn, Zn, B). The results indicated that the content of anthocyanins in leaves was not significantly different in each treatment in the beginning of treatments. The content of anthocyanin increased after 20 days with spraying Mn and Zn, and the anthocyanins content had extremely significantly positive correlation with Mn content. Fe inhibited synthesis of anthocyanin in P. persica f. atropurpurea leaves. Among phenylalanine ammonialyase(PAL), chalcone isomerase(CHI), dihydroflavonol 4-reductase (DFR) and UDP glucose flavonoid glucosyltransferase(UFGT), the change of DFR activity was more significant than PAL, CHI and UFGT, and the DFR activity after spraying Mn and Zn and the activity was significantly higher than CK. Moreover, the DFR activity increased before the anthocyanin content did, which suggested DFR initiated the synthesis of anthocyanins.


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