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Transcriptome Characterization for Camellia Sect. Oleifera Based on the 592 499 ESTs

基于油茶组59万条EST序列的转录组学初步分析


To investigate gene diversity and expression pattern in Camellia Sect. Oleifera, 454 GS FLX platform and Titanium regent were used to produce expressed sequence tags (ESTs) from flower buds of 3 Camellia species. A total of 5 920 499 ESTs were generated. Clustering those sequences twice by Cap3 produced 15 733 contigs and 20 606 singlets in C. oleifera , 19 397 contigs and 26 882 singlets in C. chekiangoleosa , 14 779 contigs and 25 389 singlets in C. brevistyla . The GC gradients in 3 transcriptomes basically followed a normal distribution. In additional all contigs and singlets were functionally categorized into GO(Gene Ontology) categories respectively.


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