Abstract:With three elements design of universal quadratic rotation combination, the models are established about the effects of N, P and K fertilizer on the relative growth rate of culm output of Phyllostachys pubescens stand. By testing, the quadratic regression medel has better simulation effect than others. The analysis of the principal effect of fertilizer shows that N, P and K have different effects on the increase of the output of P.pubescens stand, and the action order is: N>K>P in this test. 2-factor interaction analysis shows that there is a positive interaction between N and K to certain extent, but there is no significant interaction between N and P, as well P and K. By computersimulating, the optimum fertilization combination is designed (within the confidence limits of 95%), i.e.urea 325.5~413.5kg/hm2, superphosphate 20.5~295.5kg/hm2 and potassium chloride 198~267kg/hm2. Finally, the consequent economic benefit is analyzed.