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作 者 ：松毛虫病毒核酸研究课题组

期 刊 ：林业科学 1988年 24卷 1期 页码：28-33

关键词：基因；

摘 要 ：用超速离心方法分离提纯了七株松毛虫质型多角体病毒的包涵体,并对其进行了扫描电镜观察及RNA基因图谱的比较研究。JDS—CPV、JGDP—CPV、JYDPW—CPV、JADP—CPV、CYDPW—CPV五株毒株只形成一条离心沉降带。JHDS—CPV、CGDP—CPV两株均为两条离心沉降带。前五株和后两株的离心分离下带的包涵体为典型的六角形状,其RNA的3%垂直板聚丙烯酰胺、1%琼脂糖凝胶电泳图谱相同,并与家蚕CPV的电泳图谱基本一致。但日本原株JDS—CPV与其复制株的3%盘状PAGE图谱有所差异。而JHDS—CPV、CGDP—CPV株离心分离上带的包涵体为锥形体,其RNA凝胶电泳图谱为13条谱带,既不是家蚕类型,也不属于Payne,C.C.等列出的CPV—RNA基因组12种类型中的任何一种。据此,JHDS—CPV、CGDP—CPV株可能是含有两种CPV的混合株,这在国内外尚未见过报道。

Abstract:Seven strains of cytoplasmic polyhedrosis viruses of Dendrolimus spp. were isolated and pured by sucrose gradient ultracentrifugation and examined under scanning electronic microscope.A comparison study on their RNA genome segments had been made.There was only one sedimental band formed after ultracentrifugation in 5 of them-JDS-CPV,JGDP-CPV,JYDPW-CPV,JADP——CPV,and CYDPW-CPV.But there were two sedimental bands formed in both JHDS-CPV and CGDP-CPV.The shape of the cytoplasmic polyhedral bodies from the sedimental bands of the 5 CPVs and the lower sedimental bands of JHDS-CPV and CGDP-CPV appeared to be typical hexagonal.The RNA genomes extracted from the CPBs in vertical slab 3% PAGE or 1% agarosegel electrophoresis similar to Bombyx type.But there were differences between JDS-CPV in 3% disc PAGE and its replicates.The shape of the CPBs from the upper sedimental band of JHDS-CPV and CGDP-CPV appeared to be pyramidal.The RNA genome of JHDS-CPV and CGDP-CPV had 13 segments in 1% agarose gel electrophoreses and belonged to neither Bombyx type nor anyone of 12 genome types suggested by C.C.and C.F.Rivers.For this,both JHDS-CPV and CGDP-CPV might be a mixture of two strains of CPV,which has not been reported before.

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