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作 者 ：包宏 沈漫 夏民洲 胡兹苓

期 刊 ：林业科学 1997年 33卷 5期 页码：447-453

关键词：银杏；杉木；杨树；磷脂酰甘油；分子种；高效液相色谱；

Keywords:Ginkgo biloba, Chinese fir, Poplar, Phosphatiylglycerol, Molecular species, High performance liquid chromatography (HPLC),

摘 要 ：用高效液相色谱分析方法,在碳十八柱上,以乙腈-异丙醇为流动相,分析了银杏、杉木、杨树叶中磷脂酰甘油的分子种组成。分析采用磷脂酰甘油经磷脂酶C水解后,用3,5-二硝基苯甲酰氯衍生,再进行高效液相色谱分离的方法。分子种根据文献的相对保留值、标样的实验保留值、分析样HPLC峰的脂肪酸组成分析而确定。分析结果表明,三种林木叶中磷脂酰甘油均主要由八个分子种组成,在八个分子种的相对含量上,树种间存在明显差异。

Abstract:Molecular species of phosphatidylglycerol in leaves of Ginkgo biloba, Chinese fir and Poplar were analyzed by HPLC on a C₁₈ column with acetonitoile-2 -propanol (80 : 20,v / v) as mobile phase. 8 molccular species of PG were found in these 3 trees, they are (18:3/16:1(3t))PG. (18:3/16:0))PG. (18:2/16:1(3t))PG. (18:2/16:0)PG. (18:1/16:1(3t)PG, (16:0 /16:1(3t)PG, (18:1/16:0)PG, (16:0/16:0)PG, The relative amounts of these 8 molecular species in 3 bees are significantly different: (mol%) Ginkgo biloba, 30.5, 12.6, 1 7.9, 22.4, 0.2, 5.1 , 4.0, 5.3, Chinese fir, 6.4, 16.1, 1 1.5, 33.2, 0.3, 3.0, 19.8, 7.3, Poplar ,10.1, 10.6, 10.6, 14.1, 8.4, 22.3, 12.5, 11.3.

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