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作 者 ：李玉红;陈　鹏;唐爱均;王　绮;关志华;程智慧

期 刊 ：园艺学报 2005年 32卷 5期 页码：908-910

关键词：菜豆；种子老化；酶；丙二醛；POD同工酶；SDS-PAGE；

Keywords:Kidney bean, Seeds aging, Enzyme, MDA, POD, SDS-PAGE,

摘 要 ：对不同含水量的菜豆种子( 2.1%、3.4%、3.8%、6.9%和9.0% ) 老化过程中的生理特性进行了研究。结果表明, 菜豆种子在含水量为318%时, 发芽率, 保护酶系统( SOD、POD和CAT) 活性和脱氢酶活性明显高于其它含水量的种子, 而脂氧合酶(LOX) 活性和丙二醛(MDA) 含量显著低于其它含水量的种子, 表现出较高的生活力。此结果表明适度降低菜豆种子的含水量可以提高其抗老化劣变能力;不同生活力的菜豆种子POD同工酶酶谱表现不同, 而SDS2PAGE蛋白质谱带则基本相同。

Abstract:Kidney bean seeds of different moisture contents ( 2.1% , 3.4% , 3.8% , 6.9% and 9.0% ) were used to investigate the physiological characteristics in aging course. The results showed that the germination percent and the activity of SOD, POD, CAT and dehydrogenase were higher while LOX activity and MDA content were lower in 3.8%moisture content than othermoisture content seeds. It showed more vigor than other seeds. Reducing the moisture content of kidney bean seeds could increase the ability of anti-aging. The isozymes of POD were different, while the protein bandswere almost the same in kidney bean seeds of different vigor.

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