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作 者 ：程立宝;秦智伟;刘宏宇;丁国华;周秀艳

期 刊 ：园艺学报 2005年 32卷 5期 页码：840-843

关键词：黄瓜；基因；雌性系；激素；时空表达；

Keywords:Cucumber, Gene, Gynoecium, Hormone, Periods and sites expression,

摘 要 ：在cs-acs1g基因克隆的基础上进一步研究了此基因不同发育阶段表达状况, 结果表明: 没有经过生长素处理, cs-acs1g基因在苗期1～5片叶中不表达, 与利用乙烯利、赤霉素等处理的材料效果相同,使用一定浓度生长素处理之后, 此基因在两叶期开始表达, 并且随着处理的次数增加而增加; cs-acs1g基因在黄瓜不同部位表达状况不同, 在根尖、茎尖生长点表达强, 在果实和腋芽中表达很弱。因此, 黄瓜cs-acs1g基因存在不同时空表达的差异。

Abstract:Research on expression of different periods and sites was based on gene cloning. The result showed that cs-acs1g gene did not exp ress during 1 - 5 leaves without treatment of hormone, as the same time, it showed the same consequence under treatment of ETH and GA, cs-acs1g gene would exp ress at the period of two leaves with treatment of certain concentration of IAA, the intensity was accumulated with increasing frequent of IAA; cs-acs1g gene would transcript differently at various sites, the intensity which the root tips and stem and tip‘s represent was distinctlymore strong than that of fruit and axillary buds. therefore,it existed discrepancy at different periods and various sites.

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