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作 者 ：陈禅友;;汪汇东;丁　毅

期 刊 ：园艺学报 2005年 32卷 5期 页码：911-913

关键词：豇豆；长豇豆；低温胁迫；可溶性蛋白质；细胞保护酶活性；

Keywords:V igna, Asparagus bean, Chilling stress, Soluble protein, Activities of cell protective enzyme,

摘 要 ：对12个长豇豆品种幼苗进行10℃低温胁迫处理, 室温(20 ±3) ℃作对照, 5 d后测定叶片可溶性蛋白质含量和4种细胞保护酶活性。结果表明: 低温胁迫下, 6个早熟品种可溶性蛋白质含量较相应对照显著升高2.3%～8.1% , 3个晚熟品种显著降低; 8个品种SOD活性显著升高27.82% ～128.52%; 4个早熟品种APX活性显著升高10.53%～39.36% , 3个中晚熟品种略有降低。这3项指标能反映豇豆品种耐寒性, 耐寒性品种3项指标升高, 以SOD活性变化最剧烈。CAT和POD活性变化不适合作为长豇豆耐寒性鉴定指标。

Abstract:The soluble protein content ( SPC) and activities of 4 cell protective enzymes ( SOD, CAT,POD and APX) of 12 asparagus bean cultivars under 10℃chilling stress for 5 days with the control of (20 ±3) ℃ were tested. The results showed that the SPC of leaves of seedlings raised significantly by 2.3% - 8.1%
in 6 early maturing cultivars under chilling stress compared with that of relative control, whereas 3 late maturing cultivars inversed, so did the activities of SOD and APX by 27.82% - 128.52% and 10.53% - 39.36% in 8 cultivars and 4 early maturing cultivars respectively. The three indices were sensitive to chilling stress and were suitable to identify the cold resistance of asparagus bean genotypes. The cold resistant cultivars exerted their SPC and activities of SOD and APX to acclimate chilling stress. The changes of activities of CAT and POD could hardly reflect cold resistance of asparagus bean cultivars.

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