Abstract:The results from seedling inoculation on 3 accessions Lycopersicon hirsutum including LA1033, LA2099 and LA1777 proved that all of them gave a high resistance to Phytophthora infestans strains T10203. Moreover, LA2099 and LA1777 also p roduced a high resistance to strain T1020304, which was much more pathogenicity for tomato in China. F1 seeds from crossing these species with tomato lines were so wed in the greenhouse but gave very low germination rate. Significant difference was observed among different combinations, and it mainly depends on the different maternal genetic background and wild accessions.Comparing to direct sowing, mature embryo rescue in MS medium could p rovide more convenient, efficient and easy way to get F1 plants. Fruit setting of F1 pollinated by artificial pollination in the field indicated the great difference which was existed among the combinations. The above identified wild species and the introgression progenies would provide the potential use for tomato breeding resistant to Phytophthora infestans in China.