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Laboratory Bioassay Experiment of Calliptamus abbreviatus and Myrmeleotettix Palpalis After Dealing with Different Formula of Paranosema locustae and Metarhizium anisopliaeacridum

蝗虫微孢子虫和绿僵菌不同配方饵剂对短星翅蝗和宽须蚁蝗的室内生测研究



全 文 :!24" !1#
 Vol.24  No.1
! " # $
ACTA AGRESTIA SINICA
   2016$  1%
  Jan.  2016
犱狅犻:10.11733/j.issn.10070435.2016.01.023
OPJQPwRSbc~7XTÏ$UVWOw
“XYO2ZÞ²Z[
0 ä1,Åå¶1#,,æç2,,èx3,éžê3,3Í{3,ëåx1
(1.&()Y+!K*+¾¨+,,:;100193;2.&(*,JKÐÑ/01,:;100094;
3.Îëìç-.ÃcÒ,234 Îëìç027000)
78

n¡”íö?×mö
(犘犪狉犪狀狅狊犲犿犪犾狅犮狌狊狋犪犲)6•îÞ(犕犲狋犪狉犺犻狕犻狌犿犪狀犻狊狅狆犾犻犪犲‘犪犮狉犻犱狌犿’)DíöTÜ x
ö

L()ú¡¢£¤-.J±9K*+DÃ^CÒ

DC/ïí
(犆犪犾犾犻狆狋犪犿狌狊犪犫犫狉犲狏犻犪狋狌狊)6ޑðí(犕狔狉犿犲
犾犲狅狋犲狋狋犻狓狆犪犾狆犪犾犻狊)=¾\‘íö?×mö、•îÞ|ñGÎÜ?šÓù_,\‘Ë29Ã,MN28dT¾öë,Æ
×Ñ0µxö

ef…˜

÷äíö?×möÜ C/ïí

¿BíöTäd`n4.44×105 M×m,Ü :f
W65.09%。÷äíö?×möܠޑðí,¿BíöTäd`n4.44×104 M×m,Ü :fW55.64%。?
šädíö?×mö6•îÞÜ -.íö2

å™D¿BíöTäd`=¾n4.44×104 61.11×106 M,Ü:
©LWd100%。C/ï툸,íö?×möDޑðíTÜ:ó`。
9:;

íö?×mö

•îÞ

C/ïí

ޑðí

¾6Ý

0µÝ
<=>?@
:S433.2    ABCDE:A     AFG@:10070435(2016)01017107
犔犪犫狅狉犪狋狅狉狔犅犻狅犪狊狊犪狔犈狓狆犲狉犻犿犲狀狋狅犳犆犪犾犾犻狆狋犪犿狌狊犪犫犫狉犲狏犻犪狋狌狊犪狀犱
犕狔狉犿犲犾犲狅狋犲狋狋犻狓犘犪犾狆犪犾犻狊犃犳狋犲狉犇犲犪犾犻狀犵狑犻狋犺犇犻犳犳犲狉犲狀狋犉狅狉犿狌犾犪
狅犳犘犪狉犪狀狅狊犲犿犪犾狅犮狌狊狋犪犲犪狀犱犕犲狋犪狉犺犻狕犻狌犿犪狀犻狊狅狆犾犻犪犲‘犪犮狉犻犱狌犿’
ZHANGJing1,SONGLimei1#,WANGGuangjun2,WANGHuiping3,
JIANGZhongdong3,YUFengchun3,BANLiping1
(1.ColegeofAnimalScience&Technology,ChinaAgriculturalUniversity,Beijing100193,China;
2.InstituteofPlantProtection,CAAS,Beijing100094,China;3.TaipusiqiGrasslandStation,Taipusiqi,InnerMongolia027000,China)
犃犫狊狋狉犪犮狋:Inordertoinvestigatethecontrolefficiencyof犘犪狉犪狀狅狊犲犿犪犾狅犮狌狊狋犪犲and犕犲狋犪狉犺犻狕犻狌犿犪狀犻狊狅狆犾犻犪犲
var.犪犮狉犻犱狌犿onlocust,anexperimentwascarriedoutbycomparingofmortalityanddetectedinfectionaf
terfeeding犆犪犾犾犻狆狋犪犿狌狊犪犫犫狉犲狏犻犪狋狌狊犐犽狅狏狀狀.and犕狔狉犿犲犾犲狅狋犲狋狋犻狓狆犪犾狆犪犾犻狊Zub.with犘.犾狅犮狌狊狋犪犲or犕.
犪狀犻狊狅狆犾犻犪犲var.犪犮狉犻犱狌犿andthemixtureofboth,respectively,for28days.Theexperimentwascarried
outattheMinistryofAgriculturePestInvestigationStationinRangeland.Theresultsshowedthatthe
controlefficiencywas65.09%and55.64%,byonlyusing4.44×105P.locustaeevery犆.犪犫犫狉犲狏犻犪狋狌狊and
4.44×104P.犾狅犮狌狊狋犪犲every犕.犘犪犾狆犪犾犻狊,respectively.Thecontrolefficiencywas100%byutilizingthe
mixtureof4.44×104犘.犾狅犮狌狊狋犪犲and1.11×106犕.犪狀犻狊狅狆犾犻犪犲everygrasshopper.犘.犾狅犮狌狊狋犪犲hadstron
gercontrolefficiencyon犕.犘犪犾狆犪犾犻狊than犆.犪犫犫狉犲狏犻犪狋狌狊.
犓犲狔狑狅狉犱狊:犘犪狉犪狀狅狊犲犿犪犾狅犮狌狊狋犪犲;犕犲狋犪狉犺犻狕犻狌犿犪狀犻狊狅狆犾犻犪犲;犆犪犾犾犻狆狋犪犿狌狊犪犫犫狉犲狏犻犪狋狌狊;犕狔狉犿犲犾犲狅狋犲狋狋犻狓犘犪犾
狆犪犾犻狊;Mortality;Infection
HIJK
:20140925;LMJK:20141219
NOPQ

¬º˜‘)

()

*/©´
(201003079)“Å
5RST

ªñ
(1989),È,¸ ·òÂÉ,šË/09,Ì«ÍÎ-_øù?-9¤/0,Email:zj15191418070@126.com;0S†(1988
-),È,ؤnÚÉ,ÊË,Ì«ÍÎ-_ÐÑÒÓ/0,Email:songlimei2007@163.com;#D»ŠJ@?|},nÆj!ßc
™
;<Ôc™ Authorforcorrespondence,Email:lipingban@cau.edu.cn
  ð÷Sš‰_ŽT(nÜ6-.ÜTÌ«ÀÖ
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[1]。
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(犆犪犾犾犻狆狋犪犿狌狊犪犫犫狉犲狏犻犪狋狌狊Ik
ovnn.)6 Þ ‘ ð í (犕狔狉犿犲犾犲狅狋犲狋狋犻狓 狆犪犾狆犪犾犻狊
Zub.)Ú¡¢£¤¥-.íöHIG[23]。 ùí
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íö?×mö
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×möµ
(Microsporidia),?]mö*(Nosematid
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íö?×möY`KLíöTEDK
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äd11$_E§+LéBC_[19]。íö?×mö
DGíöJÜ cd
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LíöTGOë`ts
T2‹

ˆDt€T"##¾Ýkíö?×mö
LbíöGOë`ÒÓcA
[20],
I‘tsT¤×
`|6-±íöáê?H­‘’n,- ù-.
íöT9K"í¤1
[21]。
•îÞ
(犕犲狋犪狉犺犻狕犻狌犿犪狀犻狊狅狆犾犻犪犲 ‘var.犪犮狉犻
犱狌犿’)¬é—Þû(Eumycota)、mxÞ*û(Asco
mycotina)、¨ Þ Ç (Pyrenomycetes)、Ä ù Þ µ
(Sphaeriales)、^ 6Þ*(Clavicipitetaceae)、•îÞ
¬
(犕犲狋犪狉犺犻狕犻狌犿)[2223],ÚßGJ:Twö—Þ。
•îÞT=9×mŒjIìéÌÌNg

ß_§‡
Ph°9Ÿ

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<=KLED

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UV&ü
ýÀ]^

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H.ep1B
[24],
Ý."#Ì̾6

•îÞTH
ILés:

D“þ!K€Ú

Hl´µu

Ð
Ñáê?
[25]。
íö?×mö6•îÞÚµvÜ íö°±Ý
×

ŠdÝ,-T9K(Î
[26],
å™ÜJHý­

•îÞC#Ü:…†

§BgØ€ö,†A

í
ö?×mö§BLíöGO%Ÿ#J:+b

§B
SÜí¢Tÿ°

·n

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Ü:T/0tƒ

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KÜ -.íöTÝnÒâ

1 UVWXY
1.1 jP¸Af™L
ö”

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^CқœTC/ïí6ޑðí

Ë2‹DÍÖ

ÛA13~26℃,‘Ò12h,¿ù
30B。L#Gv!"24h,Äd。
1.2 ºj9,
íö?×möç”

&()Y+›œTíö
?×mö×4á

×m»`1.84×109 M·mL-1)。
•îÞç”

&()*+,9KÜ /01
›œ•îÞÞrIMI330189(×m»`1.2×109
M.g-1)。
1.3 TÏ7XÛµfèeXY
Ïd–JMNPÄmTŸ25cm,Þ19cm,s
7cmT†¸=ýù‹Díö。¿V–Ò#ý(g1)
R©=’3V,=¾>L3Mù&cn3M•*,¿
M•*J30BBö。Böç”éDÃÒIä3?
T²³

ÉMTC/ïí6ޑðí

ŒjÐBö
!"24h,·_\‘24hT‹$#ýÓù。Óù
_

‹$_xìí
(犕犲犱犻犮犪犵狅狊犪狋犻狏犪L.),¿á\‘
1“¾6öëT^²,_¯ö%,-20℃Ռ。
1.4 ²³PQfXY
f|ùBö%N

L"Â60mm /H&î1
mL°ôQ,÷B/I_,ÞáÍ&J4’!>Tü
bŒ=ì

Í1.5mL5!ø&Äd。ÏdIJ
ëP

L40±‘+…?(œDD5Mõ¾,²ßR
©¿Mõ¾×më

2×më=9

íö?×mö=9FÏ
:09,ï×m;19,
õ¾&ué1M×m;29,õ¾&J1~5M×m;
39,õ¾&J6~100M×m;49,õ¾&JYé
100 M × m;5 9,õ ¾ & × m † ¯ ’ ´ (Y
é200M)[27]。
271
!1# ª ñ?:íö?×mö6•îÞ6@–Ò#ÜDC/ïí6ޑðíTË29Ã/0
`1 ZÞ²2TV7X
Table1 Baitformulaoflaboratorybioassayexperiment
³{
Numbers
Óù
Treatments
^)
Wheatbran/g
•îÞ
犕.犪狀犻狊狅狆犾犻犪犲
1.2×109M·g-1
&dç
Oil/μL
?×mö
犘.犾狅犮狌狊狋犪犲/
1.84×109M·mL-1
C DÒControl 2 - 200 -

•îÞ
(108M×m)
犕.犪狀犻狊狅狆犾犻犪犲(108spores)
2 0.1 200 -
P1
íö?×mö
(4×106M×m)
犘.犾狅犮狌狊狋犪犲(4×106spores)
2 - 200 2
P2
íö?×mö
(4×107M×m)
犘.犾狅犮狌狊狋犪犲(4×107spores)
2 - 200 20
P1+M
íö?×mö
(4×106M×m)
+•îÞ(108M×m)
犘.犾狅犮狌狊狋犪犲(4×106spores)+
犕.犪狀犻狊狅狆犾犻犪犲(108spores)
2 0.1 200 2
P2+M
íö?×mö
(4×107M×m)
+•îÞ(108M×m)
犘.犾狅犮狌狊狋犪犲(4×107spores)
+犕.犪狀犻狊狅狆犾犻犪犲(108spores)
2 0.1 200 20
  •îÞ7×Ñ0µÝ。"ޓD¹2>1ª
ìí

Bö>LìíS

z`FM

УìíŸë

ËۓD4d,öNgÓ¤98ºw•pÞPn0µ
•îÞ

^²0µöë

1.5 lmno
1JëÏ=•©LExcel20036SAS8.2Õ
Ö&\‘

(1)¹Ö¾6Ý%=∑vnd¾6öë/cöë
×100%
(2)0µ»ë%=[(∑09¾ë×öë)/Ý
s9¾ë×cöë]×100%
(3)¾6Ý%=¾6öë/cöë×100%
(4)0µÝ%=0µöë/¾6öë×100%
(5)˜‡¾6Ý%=(Óù¾6Ý%-DÒ¾6
Ý%)/(100-DÒ¾6Ý%)×100%
jSëÏ©j\‘u‡*RÒ}ÚÛsin-1,U
dÝu…†ØëâLSDâ¸tÓù%L5%QR
STغ…†

2 pqW>r
2.1 c~7Xno2UVWOw“XYO28犱Þ
[\]^
~ë11˜,LBCT28d&,P2äÓùTC
/ïí¹Ö¾6ÝséDÒä

UhP2ÓùDC
/ïíJÜ :f
。P1ÓùTC/ïíLBCT
28d&¾6óIDÒàQ,UhP1DC/ïí
îJh…Î:
。M,P1+M 6P2+M ÓùTC/
ïíT¹Ö¾6ÝóIˆQ

òBö=¾L!8d,
!7d6!8dàú¾6,Uh•îÞDé×Ø、C
# ùC/ïí:ft`

~ë21˜,LBCT28d&,P2äÓùTÞ
‘ðí¹Ö¾6ÝLÜ ô#…†séP1äÓ
ù

I!21dj_óéˆ@,UhP2ÓùDޑð
íô#Ü:HéP1äÓù,Iñ™Ÿ#Ü :f
ˆ@
。MäÓùTޑðíT¹Ö¾6ÝL!3d
j_©séDÒä
、P1ä6P2äÓù,òޑðí
BöL!6dàú¾6,P1+Mä6P2+MäÓù
Tޑðí©L!7dàú¾6,ò.3MÓùä
T¹Ö¾6ÝóIˆQ

Uh•îÞDC#

×ØÜ
 Þ‘ðí:ft`

»BC•îÞd`As

îJ
WdùKTBC:f

2.2 c~7X$UVWOw“XYO2Œ¤_€
DBC28áTC/ïí¾6öë\‘ò^²,
_¯ö%Æ(×

efgh

g2),Óù M ä,P1
+Mä6P2+MäTC/ïíT¾6Ý6˜‡¾
6Ý©…†séP1äÓù6DÒä(犘<0.05),U
h•îÞ|‘?ääT:ft`

nP2äÓù
éXÛã%

Uh÷óädíö?×mö ùC/
ïíTäd`̓n¿90B4×107 M×m。
371
! " # $ !24"
=1 c~7Xno2UVWO28犱Þ·¸[\Õ
Fig.1 Accumulativemortalityof犆.犪犫犫狉犲狏犻犪狋狌狊underdifferenttreatmentsin28days
=2 c~7Xno2“XYO28犱Þ·¸[\Õ
Fig.2 Accumulativemortalityof犕.狆犪犾狆犪犾犻狊underdifferenttreatmentsin28days
  èg2Lã,DéC/ïí0µ.Kxö,4
MÓùäTíö?×mö0µÝ6íö?×mö0
µ»ë©ï…†Øº

•îÞ6íö?×mö@2
0µ6=¾0µC/ïíT0µÄAˆQ

Uh•
îÞ6íö?×möLC/ïíN2L§6+Lˆ
öcd

DBC28dTޑðí¾6öë\‘ò^²,
_¯ö%Æ(×

efgh

g3),D҈¸,5
GÓùÒâDޑðíT¾6Ý©Wd…†QR
(犘<0.05),Uh.}Ü Òâ©DޑðíJÜ
 :f

‘&
,Mä,P1+Mä6P2+MäÓùT
ޑðíT¾6Ý6˜‡¾6Ý©…†séP16
P2äÓù(犘<0.05),Uh•îÞDޑðíTÜ
 :ft`

èg3Lã,P2+MäÓùTޑðííö?
×möT0µ»ë34.48%,…†€éP2ÓùäT
66.15%(犘<0.05),n‘XÓù©öWd…†Q
R

ghޑðí@20µ•îÞ6íö?×mö
2

•îÞ0µÄAîJUV

íö?×möT0µ
ÄA„œóI

471
!1# ª ñ?:íö?×mö6•îÞ6@–Ò#ÜDC/ïí6ޑðíTË29Ã/0
`2 c~7Xno2UVWO2[\Õw9,Œ¤Ö_
Table2 Mortalityandpathogensinfectionof犆.犪犫犫狉犲狏犻犪狋狌狊usingdifferentformuals
³{
Numbers
¾6Ý
Mortality/%
˜‡
¾6Ý
Correctedmortality/%
•îÞ0µÝ
犕.犪狀犻狊狅狆犾犻犪犲
infection/%
?×mö
0µÝ
犘.犾狅犮狌狊狋犪犲
infection/%
?×mö0µ»ë
犘.犾狅犮狌狊狋犪犲
infection
index/%
C 68.89b - - - -
M 100.00a 100.00a 63.84a - -
P1 70.41b 39.12b - 60.35a 35.16a
P2 83.57ab 65.09ab - 49.27a 35.58a
P1+M 100.00a 100.00a 66.62a 56.91a 24.10a
P2+M 100.00a 100.00a 52.87a 59.77a 24.51a
  ü:@j6@uU;Çg˜Øº…†(犘<0.05),œ@
Note:Differentsmallettersinthesamecolumnshowsignificantdifferenceatthe0.05level,thesameasbelow
`3 c~7Xno2“XYO2[\Õw9,Œ¤Ö_
Table3 Mortalityandpathogensinfectionof犕.狆犪犾狆犪犾犻狊subjectedtodifferentformuals
³{
Numbers
¾6Ý
Mortality%
˜‡¾6Ý
Corrected
mortality%
•îÞ0µÝ
犕.犪狀犻狊狅狆犾犻犪犲
infection%
?×mö0µÝ
犘.犾狅犮狌狊狋犪犲
infection%
?×mö0µ»ë
犘.犾狅犮狌狊狋犪犲
infectionindex%
C 35.56c - - - -
M 100.00a 100.00a 61.14a - -
P1 74.77b 55.64b - 75.39a 35.46b
P2 69.02b 48.89b - 70.02ab 66.15a
P1+M 100.00a 100.00a 65.86a 66.66ab 36.50b
P2+M 100.00a 100.00a 65.67a 54.54b 34.48b
2.3 OPJQP2¾035­
ÕÛDBC_+ÉTC/ïí6ޑðí2
GíöTíö?×mö0µÝ

íö?×mö0
µ»ë\‘¸t

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Table4 Theinfectionoflive犆.犪犫犫狉犲狏犻犪狋狌狊and犕.狆犪犾狆犪犾犻狊with犘.犾狅犮狌狊狋犪犲afterexperiments
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Numbers
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Species
BC_Éöë
Livegrasshoppers
afterexperiments
?×mö0µÝ
犘.犾狅犮狌狊狋犪犲infection%
?×mö0µ»ë
犘.犾狅犮狌狊狋犪犲
infectionindex%
íö?×mö
(4×106M×m)
犘.犾狅犮狌狊狋犪犲
(4×106spores)
C/ïí
犆.犪犫犫狉犲狏犻犪狋狌狊
15 59.72a 22.78b
ޑðí
犕.狆犪犾狆犪犾犻狊
12 61.11a 45.56a
íö?×mö
(4×106M×m)
犘.犾狅犮狌狊狋犪犲
(4×106spores)
C/ïí
犆.犪犫犫狉犲狏犻犪狋狌狊
7 6.67b 1.33b
ޑðí
犕.狆犪犾狆犪犾犻狊
14 69.44a 45.56a
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”•AB
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