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Effects of Abscisic Acid and 6-Benzylaminopurine on the Drought Resistance of Vetiveria zizanioides

脱落酸和细胞分裂素对香根草抗旱性的影响



全 文 :!23" !6#
 Vol.23  No.6
! " # $
ACTA AGRESTIA SINICA
   2015$  11%
  Nov.  2015
犱狅犻:10.11733/j.issn.10070435.2015.06.013
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(1.³Å4ª()*+)-,¡É ¡1510642;2.=><)-³ÅŽ*,¡É ¡1510650)
QR

¾‹¡¡¢^Cõ¸/
(ABA)eýbxÙw(6BA)hŽ×`(犞犲狋犻狏犲狉犻犪狕犻狕犪狀犻狅犻犱犲狊)¨ t~lém,”ét
U=hŽ×`»¼%täv¾@

od¶ïABAe6BA¾@hŽ×`³oMhpŒº、MhUŲ、ßÁ
à
(MDA)e¨c]IJlém。ÆÇÈÉ:%tävÍ,Ž×`³oMhpŒºþœ,Ì ABAe6BA¾@ü³
olMhpŒºv€d^lŒã

MhUŲe MDApºÑv€dslŒã;”°T¾@!17d,ABAe6BA
¾@lŽ×`ŽBÕc]Ý]†
(SOD)、öc]©†(CAT)lC~¶‡q°O¥P(GSH)e¨,Þ/(AsA)
lpº¸!"^HØÙ¾@lŽB

¢öc]†
(POD)C~oØÙ¾@lŽBs。ÈÉ ABAe6BA¾@Â
^+Ž×`l¨t~

Ìý¨t~lÂ^A¨c]IJŒãlÂ^Ù=

STU

Ž×`

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õ¸/

ýbxÙw
VWXYZ
:S945.78    [\]^_:A     [`aZ:10070435(2015)06122005
犈犳犳犲犮狋狊狅犳犃犫狊犮犻狊犻犮犃犮犻犱犪狀犱6犅犲狀狕狔犾犪犿犻狀狅狆狌狉犻狀犲狅狀狋犺犲犇狉狅狌犵犺狋
犚犲狊犻狊狋犪狀犮犲狅犳犞犲狋犻狏犲狉犻犪狕犻狕犪狀犻狅犻犱犲狊
HUZhiqun1,FENGXueLan1,WUChubin2,ZHOUBiyan1
(1.ColegeofHorticulture,SouthChinaAgriculturalUniversity,Guangzhou,GuangdongProvince510642,China;
2.SouthChinaBotanicalGarden,Guangzhou,GuangdongProvince510650,China)
犃犫狊狋狉犪犮狋:Theeffectsofabscisicacid(ABA)and6benzylaminopurine(6BA)onthedroughtresistanceof
vetiver(犞犲狋犻狏犲狉犻犪狕犻狕犪狀犻狅犻犱犲狊)wereinvestigatedunderacontroledenvironmentgrowthchamber.The
relativewatercontent,relativeconductivity,malondiadehyde(MDA),andantioxidantsystemlevelsof
vetiverleavesweremeasuredindifferentdroughtstresstreatments.Theresultsshowedthattherelative
watercontentofthedroughttreatedleavesdecreased,whiletherelativeconductivityandMDAcontentsof
thedroughttreatedleavesincreased.Activitiesofsuperoxidedismutase(SOD)andcatalase(CAT),con
tentsofreducedglutathione(GSH)andascorbicacid(AsA)intheABAand6BAtreatedleavesweresig
nificantlyhigherthanthoseofuntreatedcontrolsaftertreatedfor17d,whereasperoxidase(POD)activity
waslowerthanthatofuntreatedcontrols.TheseresultssuggestedthatABAand6BAenhancedthe
droughtresistanceofvetiver.Theenhanceddroughtresistancemightberelatedwiththeantioxidantsys
teminducedbyABAand6BA.
犓犲狔狑狅狉犱狊:犞犲狋犻狏犲狉犻犪狕犻狕犪狀狋狅犻犱犲狊;Droughtresistance;ABA;6BA
CDEF
:20140929;GHEF:20150122
IJKL

¡É8<®Vµ†
(2013B020302005)B
MNOP

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(1966),¼,¡6í‘n…,½‡,^ {ST§,Š‹ŒŽfgAG@DE,Email:zhqhu@scau.edu.cn;‘’“
Authorsforcorrespondence,Email:wcb200811@126.com;zhoubiyan@scau.edu.cn
  Ž×`(犞犲狋犻狏犲狉犻犪狕犻狕犪狀狋狅犻犱犲狊),ê[ê£`,
¾á§<Ž×`!|$G`§Ž

qÝH:Ðe
àì(·¡5jK5j?–

Ž×`×Ié¾ú
å

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Ž×`‡Ùé¦lGHun
~e¨Œ~

hQ[!`Ùf¦lr™9

%Ì¡¢
¢Hôu

Â77Äl¼AeQ[!³€lGì
Y¿U=
[1]。
¢’¾ŒAå€e&ùY¢MlŽ
×`|G&”EQ&=

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¢”oŽz#

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¨%Ü

%FÂ^Ž×`l¨t~h»ò‰h¡Ž
×`ln¢ÿÙQ‹l®¯

‡… l D E È É

õ ¸ /
(abscisicacid,
ABA)[24]eýbxÙw[5]s˜!"Â^Žhs
t

%t¡àGävlr™9
。ABAs˜öÂ
!6# ?A·¡:õ¸/eýbxÙwhŽ×`¨t~lém
^ŽÓŸl¨c]†C~e¨c]òlŒãq
Â^¨~
[3];
ÌýbxÙws˜Â^ŽlC~c
²me9

þœC~ch¯°ålRS

íåèö
c]

倯°ålHÈ~
[6]。
¢=H ABAeý
bxÙwhŽ×`¨t~lémÅ6œ

§¨˜
étU=G&l*fŽ×`ŽB¾°½

DE+ï
¡ ABA e ! H ý b x Ù w l 6T Z U V W
(6benzylaminopurine,6BA)hŽ×`³oMhp
μ

MhUŲ

ßÁà
(MDA)pºlém,˜‹
¡I2JG&qrò¾@ðùs˜Â^Ž×`l
¨t~

§¨‡No+%tävÍABAe6BA¾
@>³o¨c]IJŒãlk]

x£¨c]IJ
Œãlk]A¨t~l=I

¾Ž¢G&qrXÂ
^Ž×`¨~ÂÃ@¢gY

1 bcdef
1.1 bc²‰
°T½)¾G&H³Å4ª()*+)-°T
Z?l
‘Sunshine’Ž×`。±u&È|ýò*,
yY`ó

xZ×M¤lŽ×`Õ

ìfæx›¾
15cmlt)*=,gßZò¾j‹Ae`Õê‡
5:1o©~°,3*U€¡ºlgßZò,3*fJ
5”,íÚ܌e¡ªÀ@,4.>‚ì•étU
(06∶00—18∶00 ¾ 28℃,18∶00—06∶00 ¾
23℃)=G&,1.>»¼˜Í°T:
¾+±ËߙlABAe6BA¾@dÐ,”%
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Ž×`ŽBx2ï¡5,10,15,20,30,50
mg·L-1l(S)ABAe6BA14,Fّ­=¾
p0.01%(v/v)Tween20。%t¾@lˆò¾ŽB
ñx܌>££»¼ÜŒ¾@

˜ï¡£pABA6BA、¢¤}pÙTween20‘­ýví܌¾@l
ŽB¾h-1(CK1),̣܌l¾%th-¾@2
(CK2)。ï¡lˆò˜ï4³‰e³ô¢£[Œ¾
Ð

”%t¾@9d(AÍpŒº»¾12%)Þ©}N
o³olMhUŲ

©¢HëÊz°Tù
,44
QY

ŽB”¤}vwÍlétU=»¼˜Í4[¾
@

³ ‰ ï ¡ 10 mg·L-1 ABA,p0.01% l
Tween20,¤Þ%t¾@;³‰ï¡15mg·L-1
6BA,p0.01%lTween20,%t¾@;%th
-¾@¾³‰ï¡p0.01%lTween20lŒ,¤
Þ%t¾@

víh-
(CK)¾³‰ïpÙ0.01%
lTween20lŒ,ví܌¾@。ï¡lˆò˜
ï4³‰e³ô¢£[Œ¾Ð

”¾@1,5,9,13,
17dNo2,3̳oMhUŲeMhpŒº,
MDA p º,Õ c ]  Ý ] † (superoxidedis
mutase,SOD),öc]©†(catalase,CAT)eöc
]†
(peroxidase,POD)C~,¨ ,Þ/(ascorbic
acid,AsA)e‡q°O¥P(reducedglutathi
one,GSH)pº。©¢HëÊz°Tù,44
QY

1.2 €ef
1.2.1 ch¡jf]¢£ ¢A͌xNo9N
oAÍlpŒº

1.2.2 îïðñòeîï¡jf¢£ MhUÅ
²eMhpŒºNo‡Zhou¡[3]lˆò。
1.2.3 óôgõöŠ¢£ SODC~No©¢¦
½"(NBT)‡qò,‡ Giannoplitis¡[7]lˆò
»¼

˜‡q50% NBT¾1[†C~ÆÌ。
CATC~lNo‡ Chang¡[8]lˆò»¼。
No1minŸ240nm¾ODIk],o¯¯cdI
3xAk]1.0¾1[†C~ÆÌ。
PODC~lNo©¢¹qü]ò,‡Kraus¡[9]
ˆò»¼

No2minŸ470nm¾ODIk],o¯¯
cdI3xAk]0.01¾1[†C~ÆÌ。
1.2.4 AsAeGSH¡f]¢£ uŽ×`³oˀ
5mL5%l•Ž/‘­D¦Âu,4℃10000×gon
15min,—²­oú•5mL,¢HNoAsAeGSH。
AsApºNoÖ-NakanoeAsada[10]lˆò,GSH
pºNoÖ-Elman[11]lˆò。
1.2.5 MDA¡f]¢£ Ö- Huang¡[12]lˆ
ò

©¢.²o^/oMò

¢x¯¯Ðx2
HG&532nme600nm¾No ODI,MDAp
º
(mM)¾(OD532OD600)/(1.55×105)。
1.3 ‚ƒlm
°TÆÇ¢ƒ]ã¸×‡:;_È6

©¢
SPSS16.0Zwx££¤dÐG&qrX¾@(<
=h-

ñù”0.05Œã—laê!"~()¥«
_YéaNT
)。
2 …†dX„
2.1 犃犅犃{6犅犃³´lmµ@õ‹
¾±ËߙlABAe6BA¾@ldÐ,œP
¦ƒNo%t9d>R£¤dÐABAe6BA¾@
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ŽBlMhUŲ

0û1(A)F6,10mg·L-1
ABA¾@ŽBlMhUŲo5mg·L-1¾@ŽB
s57.5%,oØÙïABAl%t¾@ŽB(CK2)s
69.1%,!"sHžidо@lŽB(犘<0.05),
%F

”>ç°T=

±¢10mg·L-1lABA»¼
¾@
。15mg·L-16BA¾@ŽBMhUŲx2
o5mg·L-1e10mg·L-1¾@ŽBs48.7e
33.3%,o%t¾@ŽB(CK2)s63.6%,!"s
Hžidо@lŽB
(犘<0.05),”>ç°T=,˜
15mg·L-1dл¼¾@。
W1 ()µ犃犅犃(犃){6犅犃(犅)lm/±"¤|¢Ë/¶ŒŠ@AB
Fig.1 EffectsofABA(A)and6BA(B)withdifferentconcentrationsontherelativecontentsofvetiverleaves
Ô
:CK1:ví܌h-,CK2:%th-;£¤Í±È6aê!"(犘<0.05)
Note:CK1:normalwatertreatment.CK2:droughttreatment.Differentlettersindicatesignificantdifferencesatthe0.05level
2.2 8ƒ%&犃犅犃{6犅犃lm/ #k“?
{±"¤|¢Ë/k“?@AB
0û2F6,%t¾@1~9dA͌xpº
ÍÎßî
,9d>A͌xpºsH10%,ÍÎÞ
ÐþÖ

%tvwÍ

F¾@AÍpŒºMÇ

¢³oMhpŒºÙaê

R ABAe6BA¾@
lŽBMhpŒº^H%th-ŽB

”¾@
17d,ABAe6BA¾@ŽBlMhpŒºx2
oØ Ù ï ` ¾ @ l % t Ž B ^ 119.4% e
72.2%,aêåæ!"Œã(犘<0.05),È É
ABAe6BA¾@ŽBŒxa¾UÐo%t¾
@ŽBÑ

W2 8ƒlm/ #k“? (犃){±"¤|¢Ë/k“? (犅)@AB
Fig.2 Effectsofdroughtstressonsoilwatercontents(A)andtherelativewatercontentsofvetiverleaves(B)
2.3 犃犅犃{6犅犃lm/±"¤|¢Ë/¶ŒŠ
{ 犕犇犃k?@AB
”%tvwÍ

Ž×`³oMhUŲe
MDApº]—²^Ã。”°T¾@!17d,
ABAe6BA¾@ŽBlMhUŲx2o%
t¾@ŽBs71.8%e32.0%,MDApºÑ
x2s38.1%e25.4%,aêåæ!"Œã
(犘<0.05)。ÆÇÈÉ ABAe6BA¾@Šç
þœs%tPQlMhUŲe MDApº
(law

û3)。
2221
!6# ?A·¡:õ¸/eýbxÙwhŽ×`¨t~lém
W3 犃犅犃{6犅犃lm/±"¤|¢Ë/¶ŒŠ (犃){ 犕犇犃(犅)k?@AB
Fig.3 EffectsofABAand6BAontherelativeconductivity(A)andMDAcontents(B)ofvetiverleaves
2.4 犃犅犃{6犅犃lm/±"¤|¢Ÿ £¤¥
É@AB
sû4Asu,Ê\%tUÐlËü,%t¾@l
Ž×`³oSODC~jkÍÎ,Ì6BAeABA¾@
ŽBlSODC~òxv€”Ah-MllŒã。
Œxäv‡#

Ž×`³olCATC~k]


Œxäv9d>,%t¾@lŽ×`³oCAT
C~jkÍÎ

̳‰ï¡6BAeABA>þ†C
~v€d^lŒã

Aví܌lh-Ml

”°
T¾@!17d,ABAe6BA¾@ŽBlCATC
~x2oØÙï`l%t¾@ŽB^50.0%e
38.5%(û4B),aêåæ!"Œã(犘<0.05)。
0û4CF6,”AÍ%t‡9d,PODC~k]
£(

Ê%tUÐlËžC~£š²^
,6BAe
ABA¾@lPODC~¸!"sH%t¾@l³o(犘
<0.05),Ìví܌lh-Ñòxv€”dslŒã。
2.5 犃犅犃{6犅犃lm/犃狊犃{犌犛犎k?@AB
%t¾@‡5d,AsApºk]£(;%t¾@
5d>,6BA¾@lŽ×`ŽB AsApºûü—
²
;ABAe%t¾@lAsApºjkË;°T¾
@!17d,ABAe6BA¾@ŽBlAsApºx2
o%th-^40.6%e56.3%(û5A),aêåæ
!"΋
(犘<0.05)。
§°TÆÇÈÉ

F¾@Ž×`l GSH pº
¸Ê%tävlËÚÌ]aS£¤ÞÐlk]

%
t¾@17d,6BAe ABA¾@lŽB GSH pº
x2o%th-lŽB^33.3%e41.0%(û5
B),aêåæ!"Œã(犘<0.05)。
W4 犃犅犃{6犅犃lm/±"¤|¢
犛犗犇(犃),犆犃犜(犅){犘犗犇(犆)¥É@AB
Fig.4 EffectsofABAand6BAontheSOD(A),CAT
(B)andPOD(C)activitiesofvetiverleaves
3221
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W5 犃犅犃{6犅犃lm/±"¤|¢Ÿ·¸° (犃){¹Ûn=º»¼ (犅)k?@AB
Fig.5 EffectsofABAand6BAontheAsA(A)andGSH (B)contentsofvetiverleaves
3 ™šd…š
  &ävLúlc]ävAC~cÝGe²m
lãä™æ®,Ù=
[13]。
ví&Í

ŽÓŸC
~c²mIJsüC~cdÐ鬔Þ7æóŒã
ñÍ

0SODs²moÜXsZ(Oò2 ),üñÝ]G
7öc]©
(H2O2),Ì H2O2 sWCAT e¨,Þ
/

°O¥PÍ&IJ²m
[14];
ÌlŽ¾HŒ
Þ

C~clG7±²Õö²me9ÌÅ*C~c
–—

üŽÓŸúGc]äv
[15]。
c]ävlú
«çËüèöc]’¢

Ìåèöc]ݏ MDA
eC~cçüGåû™-Qlæó

åû~Ë
»ÌPúòIÎlG@¾q

ß}Å*Žüý
[1617]。
§°TÆÇÈÉ

%tävüŽ×`³oM
hpŒºÍÎ

åèöc]ݏ MDApº²^,M
hUŲ(

åû~Ë

ŽB™ææó

ABAs˜öé¬iÏ=ø、qrӟs‘~
„e˜¶ë¨/lpº

üŽ”ävvwÍ
倈|lŒxãä

vیl@o~
[1819];ABA
‡sÂ^ӟ¨c]IJlŒã
[2021]。
F^

C
ABA¾@s˜Â^Î(犜狉犻狋犻犮狌犿犪犲狊狋犻狏狌犿)l¨
t~
[22]。
§°TÆÇÈÉ

”%tävÍ
,ABA¾
@þˎ×`³oMhpŒºlÍÎ

<˳oM
hUŲe MDApºl²^,v€ål@o~,%
ÌÂ^Ž×`l¨t~
。SODðp[!bZl†,
iÖAø]Õc¼oÜXsZlXn

Qæ²mC
~cl’¢

žC~AŽl¨Œ~O«M=

CATð²m H2O2 lQ‹åæ†,ec H2O2 xy
¾O2 e H2O,ŒÌüŽë™ H2O2 lêó,žC
~AŽl¨Œ~O«M=
。AsAðŽýbQ
‹l¨c]X

is˜‡q Oò.2 ,²mòZXsZ
(·OH)¶ H2O2。GSHðŽÓŸl]òJQ‹
l¨c]X

§DEÈÉABA¾@Â^+%tä
v͎×` SOD e CAT lC~,˜¶ AsA e
GSHlpº,ITŠ!HŽÓŸ²mC~cl¨
c]IJ
。POD`!H¨c]IJ,s˜² H2O2
‡q7 H2O。ßáG¡[23]DEÈÉ,ŒxävÍ
ABA¾@Â^PODlC~。¢§DEÆÇ!6,
ABA¾@ØÙÂ^Ž×`ŽB”%tävÍl
PODC~,IAßáGlDEÆÇ£¤,ž=lq
%Ù?»ò‰x£

ýbxÙws~»ýbxÙ

<Ë·”

ÙDE
ÈÉ

ýbxÙw`s˜Â^Žl¨Œ~
[6]。
Reguera¡[24]lDEÈÉ,%tävs˜öÂ^
Œ3
(犗狉狔狕犪狊犪狋犻狏犪)ýbxÙwlŒãüŽB”ä
vvwÍv€d^Œãlb¤]eò°7qÂ
^¨t~

¡¢^CýbxÙws˜Â^Üî
(犘犺犪狊犲狅犾狌狊狏狌犾犵犪狉犻狊)l¨t~[25],ýbxÙw‡s
˜öÂ^¨c]†lC~

þœåèöc]qÂ
^Žl¨t~
[26]。
§DE`ÈÉ
,6BA¾@Â
^Ž×`l¨t~

IA‡…lDEÆÇò*
;6
BA‡Â^¨c]IJ= SOD,CAT C~,˜¶
AsAeGSHpº,¢ØÙÂ^PODlC~。
l—F{
,ABAe6BAs˜Â^Ž×`l¨
t~
,ABAÂ^Ž×`¨t~AžÂ^¨c]IJ
=lSOD,CATC~,˜¶ AsA e GSH pºÙ
=

Á“s˜’¾w”l¨tXn¢HŽ×`lŒ
Aå€eGìY¿U

›[[1] )‡ã,c£ì,ô,¡.Ž×`”A͈AŒAå€=
l’¢
[J].5j?@,1996,16(3):265270
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pendentandabscisicacidindependentpathwaysduringabiotic
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!6# ?A·¡:õ¸/eýbxÙwhŽ×`¨t~lém
[3] ZhouB,GuoZ,LiuZ.Effectsofabscisicacidonantioxidant
systemsof犛狋狔犾狅狊犪狀狋犺犲狊犵狌犻犪狀犲狀狊犻狊underchilingstress[J].
CropScience,2005,45(2):599605
[4] DiasMC,OliveiraH,CostaA,犲狋犪犾.Improvingelmsper
formanceunderdroughtstress:Thepretreatmentwithabscisic
acid[J].EnvironmentalandExperimentalBotany,2014,100:
6473
[5] GuptaS,RashotteAM.Expressionpatternsandregulationof
SICRF3andSICRF5inresponsetocytokininandabioticstres
sesintomato(犛狅犾犪狀狌犿犾狔犮狅狆犲狉狊犻犮狌犿)[J].JournalofPlant
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
,2000,17(2):121126
[7] GiannoplitisCN,RiesSK.Superoxidedismutase:I.Occur
renceinhigherplants[J].PlantPhysiology,1977,59(2):309
314
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