全 文 :!23" !6#
Vol.23 No.6
! " # $
ACTA AGRESTIA SINICA
2015$ 11%
Nov. 2015
犱狅犻:10.11733/j.issn.10070435.2015.06.013
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QR
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(ABA)eýbxÙw(6BA)h×`(犞犲狋犻狏犲狉犻犪狕犻狕犪狀犻狅犻犱犲狊)¨ t~lém,ét
U=h×`»¼%täv¾@
,
odï¶ïABAe6BA¾@h×`³oMhpº、MhUŲ、ßÁ
à
(MDA)e¨c]IJlém。ÆÇÈÉ:%tävÍ,×`³oMhpºþ,Ì ABAe6BA¾@ü³
olMhpºvd^lã
,
MhUŲe MDApºÑvdslã;°T¾@!17d,ABAe6BA
¾@l×`BÕc]Ý]
(SOD)、öc]©(CAT)lC~¶q°O¥P(GSH)e¨,Þ/(AsA)
lpº¸!"^HØÙ¾@lB
,
¢öc]
(POD)C~oØÙ¾@lBs。ÈÉ ABAe6BA¾@Â
^+×`l¨t~
,
Ìý¨t~lÂ^A¨c]IJãlÂ^Ù=
。
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:S945.78 [\]^_:A [`aZ:10070435(2015)06122005
犈犳犳犲犮狋狊狅犳犃犫狊犮犻狊犻犮犃犮犻犱犪狀犱6犅犲狀狕狔犾犪犿犻狀狅狆狌狉犻狀犲狅狀狋犺犲犇狉狅狌犵犺狋
犚犲狊犻狊狋犪狀犮犲狅犳犞犲狋犻狏犲狉犻犪狕犻狕犪狀犻狅犻犱犲狊
HUZhiqun1,FENGXueLan1,WUChubin2,ZHOUBiyan1
(1.ColegeofHorticulture,SouthChinaAgriculturalUniversity,Guangzhou,GuangdongProvince510642,China;
2.SouthChinaBotanicalGarden,Guangzhou,GuangdongProvince510650,China)
犃犫狊狋狉犪犮狋:Theeffectsofabscisicacid(ABA)and6benzylaminopurine(6BA)onthedroughtresistanceof
vetiver(犞犲狋犻狏犲狉犻犪狕犻狕犪狀犻狅犻犱犲狊)wereinvestigatedunderacontroledenvironmentgrowthchamber.The
relativewatercontent,relativeconductivity,malondiadehyde(MDA),andantioxidantsystemlevelsof
vetiverleavesweremeasuredindifferentdroughtstresstreatments.Theresultsshowedthattherelative
watercontentofthedroughttreatedleavesdecreased,whiletherelativeconductivityandMDAcontentsof
thedroughttreatedleavesincreased.Activitiesofsuperoxidedismutase(SOD)andcatalase(CAT),con
tentsofreducedglutathione(GSH)andascorbicacid(AsA)intheABAand6BAtreatedleavesweresig
nificantlyhigherthanthoseofuntreatedcontrolsaftertreatedfor17d,whereasperoxidase(POD)activity
waslowerthanthatofuntreatedcontrols.TheseresultssuggestedthatABAand6BAenhancedthe
droughtresistanceofvetiver.Theenhanceddroughtresistancemightberelatedwiththeantioxidantsys
teminducedbyABAand6BA.
犓犲狔狑狅狉犱狊:犞犲狋犻狏犲狉犻犪狕犻狕犪狀狋狅犻犱犲狊;Droughtresistance;ABA;6BA
CDEF
:20140929;GHEF:20150122
IJKL
:
¡É8<®Vµ
(2013B020302005)B
MNOP
:
?A·
(1966),¼,¡6ín
,½,^ {ST§,fgAG@DE,Email:zhqhu@scau.edu.cn;
Authorsforcorrespondence,Email:wcb200811@126.com;zhoubiyan@scau.edu.cn
×`(犞犲狋犻狏犲狉犻犪狕犻狕犪狀狋狅犻犱犲狊),ê[ê£`,
¾á§<×`!|$G`§
,
qÝH:Ðe
àì(·¡5jK5j?
。
×`×Ié¾ú
å
,
Ùf¦l¼Ae9
;
×`Ùé¦lGHun
~e¨~
,
hQ[!`Ùf¦lr9
,
%Ì¡¢
¢Hôu
、
Â77Äl¼AeQ[!³lGì
Y¿U=
[1]。
¢¾Aåe&ùY¢Ml
×`|G&EQ&=
,
_7Ùf¦l¨~
,
¢oz#
,
xpYð§oB`CAùl=
¨%Ü
,
%FÂ^×`l¨t~h»òh¡
×`ln¢ÿÙQl®¯
。
l D E È É
,
õ ¸ /
(abscisicacid,
ABA)[24]eýbxÙw[5]s!"Â^hs
t
、
%t¡àGävlr9
。ABAsöÂ
!6# ?A·¡:õ¸/eýbxÙwh×`¨t~lém
^Ól¨c]C~e¨c]òlãq
Â^¨~
[3];
ÌýbxÙwsÂ^lC~c
²me9
,
þC~ch¯°ålRS
,
íåèö
c]
,
寰ålHÈ~
[6]。
¢=H ABAeý
bxÙwh×`¨t~lémÅ6
。
§¨
étU=G&l*f×`B¾°½
,
DE+ï
¡ ABA e ! H ý b x Ù w l 6T Z U V W
(6benzylaminopurine,6BA)h×`³oMhp
º
、
MhUŲ
、
ßÁà
(MDA)pºlém,
¡I2JG&qrò¾@ðùsÂ^×`l
¨t~
;
§¨No+%tävÍABAe6BA¾
@>³o¨c]IJãlk]
,
x£¨c]IJ
ãlk]A¨t~l=I
,
¾¢G&qrXÂ
^×`¨~ÂÃ@¢gY
。
1 bcdef
1.1 bc²
°T½)¾G&H³Å4ª()*+)-°T
Z?l
‘Sunshine’×`。±u&Ã|ýò*,
yY`ó
、
xZ×M¤l×`Õ
,
ìfæx¾
15cmlt)*=,gßZò¾jAe`Õê
5:1o©~°,3*U¡ºlgßZò,3*fJ
5,íÚÜe¡ªÀ@,4.>ìétU
(06∶00—18∶00 ¾ 28℃,18∶00—06∶00 ¾
23℃)=G&,1.>»¼Í°T:
¾+±ËßlABAe6BA¾@dÐ,%
t¾@
,
×`Bx2ï¡5,10,15,20,30,50
mg·L-1l(S)ABAe6BA14,FÙ=¾
p0.01%(v/v)Tween20。%t¾@lò¾B
ñxÜ>££»¼Ü¾@
。
ï¡£pABA6BA、¢¤}pÙTween20ývíܾ@l
B¾h-1(CK1),Ì£Ül¾%th-¾@2
(CK2)。ï¡lòï4³e³ô¢£[¾
Ð
。
%t¾@9d(AÍpº»¾12%)Þ©}N
o³olMhUŲ
。
©¢HëÊz°Tù
,44
QY
。
B¤}vwÍlétU=»¼Í4[¾
@
:
³ ï ¡ 10 mg·L-1 ABA,p0.01% l
Tween20,¤Þ%t¾@;³ï¡15mg·L-1
6BA,p0.01%lTween20,%t¾@;%th
-¾@¾³ï¡p0.01%lTween20l,¤
Þ%t¾@
;
víh-
(CK)¾³ïpÙ0.01%
lTween20l,víܾ@。ï¡lò
ï4³e³ô¢£[¾Ð
。
¾@1,5,9,13,
17dNo2,3̳oMhUŲeMhpº,
MDA p º,Õ c ] Ý ] (superoxidedis
mutase,SOD),öc]©(catalase,CAT)eöc
]
(peroxidase,POD)C~,¨ ,Þ/(ascorbic
acid,AsA)eq°O¥P(reducedglutathi
one,GSH)pº。©¢HëÊz°Tù,44
QY
。
1.2 ef
1.2.1 ch¡jf]¢£ ¢AÍxNo9N
oAÍlpº
。
1.2.2 îïðñòeîï¡jf¢£ MhUÅ
²eMhpºNoZhou¡[3]lò。
1.2.3 óôgõö¢£ SODC~No©¢¦
½"(NBT)qò, Giannoplitis¡[7]lò
»¼
。
q50% NBT¾1[C~ÆÌ。
CATC~lNo Chang¡[8]lò»¼。
No1min240nm¾ODIk],o¯¯cdI
3xAk]1.0¾1[C~ÆÌ。
PODC~lNo©¢¹qü]ò,Kraus¡[9]
ò»¼
,
No2min470nm¾ODIk],o¯¯
cdI3xAk]0.01¾1[C~ÆÌ。
1.2.4 AsAeGSH¡f]¢£ u×`³oË
5mL5%l/D¦Âu,4℃10000×gon
15min,²oú5mL,¢HNoAsAeGSH。
AsApºNoÖ-NakanoeAsada[10]lò,GSH
pºNoÖ-Elman[11]lò。
1.2.5 MDA¡f]¢£ Ö- Huang¡[12]l
ò
。
©¢.²o^/oMò
,
¢x¯¯Ðx2
HG&532nme600nm¾No ODI,MDAp
º
(mM)¾(OD532OD600)/(1.55×105)。
1.3 lm
°TÆÇ¢]ã¸×:;_È6
,
©¢
SPSS16.0Zwx££¤dÐG&qrX¾@(<
=h-
)
ñù0.05ãlaê!"~()¥«
_YéaNT
)。
2
dX
2.1 犃犅犃{6犅犃³´lmµ@õ
¾±ËßlABAe6BA¾@ldÐ,P
¦No%t9d>R£¤dÐABAe6BA¾@
1221
! " # $ !23"
BlMhUŲ
。
0û1(A)F6,10mg·L-1
ABA¾@BlMhUŲo5mg·L-1¾@B
s57.5%,oØÙïABAl%t¾@B(CK2)s
69.1%,!"sHidо@lB(犘<0.05),
%F
,
>ç°T=
,
±¢10mg·L-1lABA»¼
¾@
。15mg·L-16BA¾@BMhUŲx2
o5mg·L-1e10mg·L-1¾@Bs48.7e
33.3%,o%t¾@B(CK2)s63.6%,!"s
Hidо@lB
(犘<0.05),>ç°T=,
15mg·L-1dл¼¾@。
W1 ()µ犃犅犃(犃){6犅犃(犅)lm/±"¤|¢Ë/¶@AB
Fig.1 EffectsofABA(A)and6BA(B)withdifferentconcentrationsontherelativecontentsofvetiverleaves
Ô
:CK1:víÜh-,CK2:%th-;£¤Í±È6aê!"(犘<0.05)
Note:CK1:normalwatertreatment.CK2:droughttreatment.Differentlettersindicatesignificantdifferencesatthe0.05level
2.2 8%&犃犅犃{6犅犃lm/ #k?
{±"¤|¢Ë/k?@AB
0û2F6,%t¾@1~9dAÍxpº
ÍÎßî
,9d>AÍxpºsH10%,ÍÎÞ
ÐþÖ
。
%tvwÍ
,
F¾@AÍpºMÇ
,
¢³oMhpºÙaê
,
R ABAe6BA¾@
lBMhpº^H%th-B
。
¾@
17d,ABAe6BA¾@BlMhpºx2
oØ Ù ï ` ¾ @ l % t B ^ 119.4% e
72.2%,aêåæ!"ã(犘<0.05),È É
ABAe6BA¾@Bxa¾UÐo%t¾
@BÑ
。
W2 8lm/ #k? (犃){±"¤|¢Ë/k? (犅)@AB
Fig.2 Effectsofdroughtstressonsoilwatercontents(A)andtherelativewatercontentsofvetiverleaves(B)
2.3 犃犅犃{6犅犃lm/±"¤|¢Ë/¶
{ 犕犇犃k?@AB
%tvwÍ
,
×`³oMhUŲe
MDApº]²^Ã。°T¾@!17d,
ABAe6BA¾@BlMhUŲx2o%
t¾@Bs71.8%e32.0%,MDApºÑ
x2s38.1%e25.4%,aêåæ!"ã
(犘<0.05)。ÆÇÈÉ ABAe6BA¾@ç
þs%tPQlMhUŲe MDApº
(law
(
û3)。
2221
!6# ?A·¡:õ¸/eýbxÙwh×`¨t~lém
W3 犃犅犃{6犅犃lm/±"¤|¢Ë/¶ (犃){ 犕犇犃(犅)k?@AB
Fig.3 EffectsofABAand6BAontherelativeconductivity(A)andMDAcontents(B)ofvetiverleaves
2.4 犃犅犃{6犅犃lm/±"¤|¢ £¤¥
É@AB
sû4Asu,Ê\%tUÐlËü,%t¾@l
×`³oSODC~jkÍÎ,Ì6BAeABA¾@
BlSODC~òxvAh-Mllã。
xäv#
,
×`³olCATC~k]
。
xäv9d>,%t¾@l×`³oCAT
C~jkÍÎ
,
̳ï¡6BAeABA>þC
~vd^lã
,
AvíÜlh-Ml
。
°
T¾@!17d,ABAe6BA¾@BlCATC
~x2oØÙï`l%t¾@B^50.0%e
38.5%(û4B),aêåæ!"ã(犘<0.05)。
0û4CF6,AÍ%t9d,PODC~k]
£(
,
Ê%tUÐlËC~£²^
,6BAe
ABA¾@lPODC~¸!"sH%t¾@l³o(犘
<0.05),ÌvíÜlh-Ñòxvdslã。
2.5 犃犅犃{6犅犃lm/犃狊犃{犌犛犎k?@AB
%t¾@5d,AsApºk]£(;%t¾@
5d>,6BA¾@l×`B AsApºûü
²
;ABAe%t¾@lAsApºjkË;°T¾
@!17d,ABAe6BA¾@BlAsApºx2
o%th-^40.6%e56.3%(û5A),aêåæ
!"ã
(犘<0.05)。
§°TÆÇÈÉ
,
F¾@×`l GSH pº
¸Ê%tävlËÚÌ]aS£¤ÞÐlk]
,
%
t¾@17d,6BAe ABA¾@lB GSH pº
x2o%th-lB^33.3%e41.0%(û5
B),aêåæ!"ã(犘<0.05)。
W4 犃犅犃{6犅犃lm/±"¤|¢
犛犗犇(犃),犆犃犜(犅){犘犗犇(犆)¥É@AB
Fig.4 EffectsofABAand6BAontheSOD(A),CAT
(B)andPOD(C)activitiesofvetiverleaves
3221
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W5 犃犅犃{6犅犃lm/±"¤|¢·¸° (犃){¹Ûn=º»¼ (犅)k?@AB
Fig.5 EffectsofABAand6BAontheAsA(A)andGSH (B)contentsofvetiverleaves
3 d
&ävLúlc]ävAC~cÝGe²m
lãäæ®,Ù=
[13]。
ví&Í
,
ÓC
~c²mIJsüC~cdÐé¬Þ7æóã
ñÍ
,
0SODs²moÜXsZ(Oò2 ),üñÝ]G
7öc]©
(H2O2),Ì H2O2 sWCAT e¨,Þ
/
—
°O¥PÍ&IJ²m
[14];
Ìl¾H
Þ
,
C~clG7±²Õö²me9ÌÅ*C~c
,
üÓúGc]äv
[15]。
c]ävlú
«çËüèöc]¢
,
Ìåèöc]Ý MDA
eC~cçüGåû-Qlæó
,
åû~Ë
»ÌPúòIÎlG@¾q
,
ß}Å*üý
[1617]。
§°TÆÇÈÉ
,
%tävü×`³oM
hpºÍÎ
,
åèöc]Ý MDApº²^,M
hUŲ(
,
åû~Ë
,
Bææó
。
ABAsöé¬iÏ=ø、qrÓs~
e¶ë¨/lpº
,
üävvwÍ
å|lxãä
,
vål@o~
[1819];ABA
sÂ^Ó¨c]IJlã
[2021]。
F^
,
C
ABA¾@sÂ^Î(犜狉犻狋犻犮狌犿犪犲狊狋犻狏狌犿)l¨
t~
[22]。
§°TÆÇÈÉ
,
%tävÍ
,ABA¾
@þË×`³oMhpºlÍÎ
,
<˳oM
hUŲe MDApºl²^,vål@o~,%
ÌÂ^×`l¨t~
。SODðp[!bZl,
iÖAø]Õc¼oÜXsZlXn
,
Qæ²mC
~cl¢
,
C~Al¨~O«M=
。
CATð²m H2O2 lQåæ,ec H2O2 xy
¾O2 e H2O,Ìüë H2O2 lêó,C
~Al¨~O«M=
。AsAðýbQ
l¨c]X
,
isq Oò.2 ,²mòZXsZ
(·OH)¶ H2O2。GSHðÓl]òJQ
l¨c]X
。
§DEÈÉABA¾@Â^+%tä
vÍ×` SOD e CAT lC~,¶ AsA e
GSHlpº,IT!HÓ²mC~cl¨
c]IJ
。POD`!H¨c]IJ,s² H2O2
q7 H2O。ßáG¡[23]DEÈÉ,xävÍ
ABA¾@Â^PODlC~。¢§DEÆÇ!6,
ABA¾@ØÙÂ^×`B%tävÍl
PODC~,IAßáGlDEÆÇ£¤,=lq
%Ù?»òx£
。
ýbxÙws~»ýbxÙ
、
<Ë·
,
ÙDE
ÈÉ
,
ýbxÙw`sÂ^l¨~
[6]。
Reguera¡[24]lDEÈÉ,%tävsöÂ^
3
(犗狉狔狕犪狊犪狋犻狏犪)ýbxÙwlãüBä
vvwÍvd^ãlb¤]eò°7qÂ
^¨t~
,
¡¢^CýbxÙwsÂ^Üî
(犘犺犪狊犲狅犾狌狊狏狌犾犵犪狉犻狊)l¨t~[25],ýbxÙws
öÂ^¨c]lC~
,
þåèöc]qÂ
^l¨t~
[26]。
§DE`ÈÉ
,6BA¾@Â
^×`l¨t~
,
IA
lDEÆÇò*
;6
BAÂ^¨c]IJ= SOD,CAT C~,¶
AsAeGSHpº,¢ØÙÂ^PODlC~。
lF{
,ABAe6BAsÂ^×`l¨
t~
,ABAÂ^×`¨t~AÂ^¨c]IJ
=lSOD,CATC~,¶ AsA e GSH pºÙ
=
,
Ás¾wl¨tXn¢H×`l
AåeGìY¿U
。
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