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Evaluation Method and Screening of Drought Tolerance of Elite Germplasm in the Early Growth Stage of Sudangrass

苏丹草生长早期耐旱性评价方法及种质耐旱性筛选



全 文 :!23" !5#
 Vol.23  No.5
! " # $
ACTA AGRESTIA SINICA
   2015$  9%
  Sep.  2015
犱狅犻:10.11733/j.issn.10070435.2015.05.016
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/ 01,;1.1,23<2,Q 41,56ä1,I781
(1.cô(+0 (+8´†FŽ+0 Å9ÁÂTYC:=Vº,cô300384;
2.+¬()°+˜™0 O,¹-˜™],4¥100193)
45

T˜™B¥ HoaglandáânD{Ó,}PEG8000GKz`a9,ð„H}ÅN(犛狅狉犵犺狌犿狊狌犱犪狀犲狀狊犲):u
&#þýg¥ôNÓ

Ã¥ôH22„}ÅNYù‘Bþýg。lmPJ:Wþý‡EQ‘06222’âREB
PEG8000BvìܨG-0.675MPa。ÉPEG8000BܨG-0.600=-0.700MPa“,22„}ÅNYù‘
•ƒK”P5

ƒKì†ƒKñ‚¤Üùë3egh‚BDo–Ä×rKL。|}þý«p,¦22„}ÅN
Yù‘zG5y,Ò+,‘‚(1™’、‘89105’†‘TS185’Bþýgv›,ˆ‘06222’vã。ƒK”P5=ƒKñ
‚¤Üùë–8ƒKì“”•š›Bƒ@g



K}ÅN;i:u&#þýg¥ô“PEG8000BvìÜ
¨ž•-0.675MPaÞß;•¥ô}ÅN&#Bþýg“>?ƒK”P5=ƒKñ‚¤Üùë2eghî|。
678

}ÅN

Yù‘

þýg

:u&#
;PEG8000
9:;<=
:Q945.78    >?@AB:A     >CD=:10070435(2015)05100706
犈狏犪犾狌犪狋犻狅狀犕犲狋犺狅犱犪狀犱犛犮狉犲犲狀犻狀犵狅犳犇狉狅狌犵犺狋犜狅犾犲狉犪狀犮犲狅犳犈犾犻狋犲
犌犲狉犿狆犾犪狊犿犻狀狋犺犲犈犪狉犾狔犌狉狅狑狋犺犛狋犪犵犲狅犳犛狌犱犪狀犵狉犪狊狊
GUIZhi1,GAOJianming1,YUANQinghua2,
LUOFeng1,PEIZhongyou1,SUNShoujun1
(1.TheKeyLaboratoryofCropGeneticsandBreeding,ColegeofAgronomy,ResourcesandEnvironmentalScience,
TianjinAgriculturalUniversity,Tianjin300384,China;
2.InstituteofAnimalScience,ChineseAcademyofAgriculturalSciences,Beijing100193,China)
犃犫狊狋狉犪犮狋:AsandcultureexperimentwithHoaglandsolutionplusPEG8000wasconductedtoevaluatethe
droughttoleranceof22sudangrassgenotypesintheearlygrowthstages.Theresultsshowedthataweak
droughttolerantgenotype,‘06222’,couldtolerateuptoPEG8000of-0.675MPa.UnderthePEG
8000of-0.600and-0.700MPa,therelativelivability(犚犔),relativedriedmass(犚犇犕)andrelative
plantheight(犚犎)ofsudangrassweresignificantdifferentamongthe22genotypes.Accordingtodrought
toleranceindex(犇犜犐),22studiedgenotypesweregeneralyclassifiedintofivegroups.‘Neinong1’,‘89
105’and‘TS185’genotypeswerethemostdroughttolerancecomparedtoothers.Correlationanalysis
suggestedboth犚犔and犚犇犕 possessedastrongcorrelationwith犚犎.Inconclusion,inevaluatingthe
droughttoleranceofsudangrassgenotype,themaximumconcentrationofPEG8000wasabout-0.675
MPaandtwotraits,犚犔and犚犇犕,couldbeusedtoinvestigatethedroughttoleranceofgenotypes.
犓犲狔狑狅狉犱狊:犛狅狉犵犺狌犿狊狌犱犪狀犲狀狊犲;Germplasm;Droughttolerance;Theearlygrowthstage;PEG8000
EFGH
:20140714;IJGH:20150317
KLMN

¬­°•ÉÊÁز³
(2011BAD17B01);cô¶ì!+—›ZÁí{âÁØ(TD125017);cô¶°•ÉÊÁز³
(12ZCZDNC00100)´ µ
OPQR

“·
(1973),Ë,Ü3(Þ»,¼½,ǖ½,¾D¿Àó¥Å9ÁÂTY˜™,Email:guizhi73@163.com;ÇÈÅÆ Author
ofcorrespondence,Email:sunshoujun2001@yahoo.com.cn
  Üý5KL():úB¾DE7’Š,EÜý
\áB()9“ƒÉ¦|ÁÒ~m:9®¯îÎáB9“’=
[1]。
d–|Áðñnß

•Š÷ñÝ
ÜýA_)…)RSñ@:

/¨q)…)UC

Y8þýÅ9]GY5»ÓžKÜý`aB\ŒN
Ó

ˆåýYù´†BFk†*¥5KÅ9åýg
;iÁ¿ö

ŒTåýGYB0Î=‡Ã
[2]。
}ÅN
(犛狅狉犵犺狌犿狊狌犱犪狀犲狀狊犲)GßîQŠ$:
ÉT°,N

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(犛狅狉犵犺狌犿狊狌犱犪狀犲狀狊犲× 犛狅狉犵犺狌犿犫犻犮狅犾狅狉)]?
À
[35]。
t¯}ÅNTñŒš›Bþýg

wÜý
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Uk
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WøáTšì

ˆ‰‘ù}ÖL

lmB|#gšÚ

ˆ•_º‚
¦}ÅNB:u&#;iFk|}šïñÖL‘

UVlmƒKŸÇk

UVu#qšå

LJPEG
Rà!Nd;iþýg˜™©./ž¥•C‹Å9
‚
[612]。
t¯ÐYNӕìÕO
(犛狅狉犵犺狌犿)89+©
ÕÅHž¥
[1315],
wŸ¾ð„þýg¥ôNÓ


™ƒ•ð„‡¦PEG8000=áânD{ÓBí¥
¦}ÅN¨×ìÕO89:u&#þýgFkBN
Ó

ÃB¥]ð„BNÓK22„}ÅNYù‘;
iþýgFk

G;ŠŒUk}ÅNþýgB‡Ã˜
™

GYŒT†}ÅNBŽ{XΏš>

1 STYUV
1.1 ]^ST
U}ÅNYù‘ƒ22„。Ò+,TáGY
6„,®öA11„,Ò~5„ (P1)。
1.2 犘犈犌8000¹$0¼g
CUVlm—ó

GY
‘SOR’Bþýgì,ˆ
‘06222’BþýgÚ,E£Œ¥Ð2eGY;i‹
ePEG8000ܨš:u&#BþýUV,}µk
K}ÅN;iþýg¥ôFk“í1BPEG8000
ܨ

UVB¥ÉŽÓ

‡ùG&D

S|dݾ
Á

”^XCM3ü。Ñ¥PEG8000(‚3:.)Å
GKz`a9

¥Ì1.17g·L-1B Hoaglandáâ
n;i¹L

PGE8000Bܨæ0(CK),87,124,153,178,
200=221g·kg-1ƒ7eKL,(Á.,KžB
PEG8000knB!NäÔüG-0.175,-0.275,
-0.375,-0.475,-0.575,-0.675=-0.775
MPa[16]。Œ\UVŒïCš:
¥Y7ïŒS¨ðKUB}ÅNY7;i<
Œ
,¯
ÌB¥{âÆÓjk@*5

Á.1GYBÿ
Yp³

}ûPK}B{pG”ñ40。
¦&D‡§

ªØ1mm),¥®…KòS,/
Ü

xD750gëlœ‘ñ(12cm×12cm×5cm)
+

 lWXB^Xn150mL(,G&Dv*üK
ëB90%),ŸZ2w“ÑÒæC–W。
xŠkp³BY7–WÿY

ߨ1cm。
ÿYSá̦_º+;i®¯±}{T

¦!
8c=!15cä  Hoaglandáân2ü,”ü50
mL。:u#“”ck“]x”eœ‘ñBùë,ï
5Bùë¥óÕKäÍ

!21c“ÀÁ”P{p,¦ðn{Bñ‚¤z
˜šÃ|}¿wÅ*B›ºÕÄ

Œx+“5j
뜝ì¨



¦Ò/יCÌjëi{ùë

xL–õÅGœBjkõ

”P{B¬»G

+

©QJ—BEÎòh

©5”P

×51®´©

©®‡TkU
,1/2}‚/ÂGðn´Þ)。
1.3 ¸¹3þÿ
WXYù‘Bþýg¥ôÑ¥0(CK)、2e
PEG8000ܨ(185=205g·kg-1),!NäzÆ
G-0.175,-0.600=-0.700MPa,”^XCM2
ü

Ò~80sUVNӃX

1.4 ¸¹33phijk
UVƒÀÁ3egh,îƒK”P5(relative
livability,犚犔)、ƒKñ‚¤eùë(relativedried
mass,犚犇犕)=ƒKì(relativeplantheight,
犚犎),ÒÁ.ªÂCš:
ƒKõ=100%×u^XBjkõ/K}Bjkõ
•uPEG8000ܨšU‘Bþýg¥þ
ý«p
(droughttoleranceindex,犇犜犐)Pó,Á.C
š

˦1ghBƒKõ¬»<
(Zscore),¯ ̌2
e]3eghB¬»<õƒ ÕÅþý«p。
]p½^X–B¥SPSS11.5.0;i。
8001
!5# “ ·!:}ÅN:u&#þýg¥ôNӆYùþýg§Œ
\1 ]^022½¶æ(„…ST
Table1 Tested22sudangrassgenotypes
‡EQ
Genotype
Y7…†
Seedprovider
yQ
Type
‡EQ
Genotype
Y7…†
Seedprovider
yQ
Type
SS2 a }ÅNTáGY(mÞ+E) 10-17 c }ÅN®öA
Enroma a }ÅNTáGY 1539 c }ÅN®öA
Brasero a }ÅNTáGY 85-46 c }ÅN®öA
06-222 b }ÅN®öA 89-103 c }ÅN®öA
07-41 b }ÅN®öA 89-104 c }ÅN®öA
07-54 b }ÅN®öA 89-105 c }ÅN®öA
SOR b }ÅN®öA 2680 c N±}ÅN
TS185 b }ÅN®öA 86-394 c ìÕ×}ÅNNöÌ?
Z}2™ b }ÅNTáGY 10-98 c ìÕ×}ÅNNöÌ?
·RÚ2™ c }ÅNTáGY 81-25 c ìÕ×}ÅNNöÌ?
‚(1™ c }ÅNTáGY 1540 c óN×}ÅNNöÌ?
  ò(Note):a©**Ð(L˜Y7ªe(PacificSeedsAustralia);bcô(+0(TianjinAgriculturalUniversity);c+¬()°+˜™0



O,¹-˜™]
(InstituteofAnimalScience,ChineseAcademyofAgriculturalScience)
2 lmY;n
2.1 ¶æ(b¢·H3pû‡ˆ犘犈犌8000¹$
¾0qŠ
C×1]ó,þý‡EQ‘SOR’=Wþý‡E
Q
‘06222’B3eghõ–d!NäBÊÚ(PEG
8000ܨBeì)ˆÊÚ,wÌƚʶ¨š¸。


þý‡EQ
‘SOR’•”e!NäšB3eg
hõ–ì¦ÌÆBKžõ

‰d–!NäBÊÚ

K
žõ’“BDo)*

•!Näv*“
(-0.775
MPa),þý‡EQ‘SOR’B犚犔,犚犎 =犚犇犕 zÆ
G55.1%,34.8%=26.7%;ˆWþý‡EQ‘06
222’–G0。Wþý‡EQ‘06222’•!NäG
-0.675MPa“,犚犔,犚犎 =犚犇犕 zÆG19.0%,
9.2%=11.8%。ÐPJþý‡EQ]âREBv
Ú!NäÚ¦-0.775MPa(221g·kg-1),ˆWþ
ý‡EQ]âREBvÚ!Nä•-0.679MPa
Þß
(201g·kg-1)。
¿×1ø|}Õ,¥WXܨBPEG^X}
ÅN‘

É!NäG-0.600~-0.700MPa(185g
·kg-1~205g·kg-1)“,3egh“BDoJ—,P
JTUV]Œ¥BPEG8000BܨŸG@X。
2.2 ¾3p0|63
K22e‡EQ•2ePEG8000ܨšBÙ
ghõ;iHƒ@z{
(Pearsonƒ@)。lmP
J

]áKBƒ@Ap–ÄÅH×rKL

w
犚犔8犚犇犕 •犘犈犌 ܨG-0.700MPa“Bƒ@
ApšÚ

P2)。`\…~,犚犔=犚犇犕 8犚犎 “
–”•š›Bƒ@g

îÐ2eghËÌH犚犎 B
*¤zȶ



•K}ÅNBþýg;i¥ô
“ø>?犚犔=犚犇犕 Ð2egh,ï5H.Åë
ø‡T‚W67¥ôlm

:1 ‡ˆ¹$犘犈犌jk¾¶æ(3¿3p0qŠ
Fig.1 Changesofthreetraitsofsudangrassunder
differentPEGconcentrationtreatments
ò
(Note):a,‘SOR’—RL;b,‘SOR’—RH;c,‘SOR’—RDM;
d,‘06-222’—RL;e,‘06-222’—RH;f,‘06-222’—RDM
2.3 ¶æ(„…ST¸¹30þÿ
NDz{PJ

É PEG ܨG-0.600=
-0.700MPa“,22„ìÕYù‘B犚犔,犚犎
=犚犇犕 BDo–Ä×rKL(犘<0.01),
;WX}ÅNYù‘BþýgWX

P3)。
ëP4|ì,•2ePEGܨš,‘‚(1™’B
犚犔–Gvì,ˆ‘06222’–GvÚ;犘犈犌ܨG
-0.700MPa“,]‡EQB犚犔B–õÈÜ
¨G-0.600MPa“ÊÚH14.1%。K¦犚犎,
9001
! " # $ !23"
‘8546’•2eܨš–Gvì,ˆ‘06222’–
GvÚ
;犘犈犌ܨG-0.700MPa“,]‡E
QBL–õÈܨG-0.600 MPa“ÊÚH
18.2%。•2 e ^ X Ü ¨ š,‘‚ ( 1 ™’=
‘1539’B 犚犇犕 zÆvì,ˆ‘06222’–Gv
Ú

É犘犈犌ܨG-0.700MPa“,]‡EQ
B犚犇犕 B–õÈܨG-0.600MPa“ÊÚH
6.2%。
\2 ó;©ª¾¶æ(b¢·H3¿3p0|63
Table2 Correlationof3traitsofsudangrassatseedlingstageunderdroughtstress
PEGܨ
PEGconcentration/MPa
ƒK”P5
Relativelivability/%
ƒKì
Relativeplantheight/cm
ƒKì
Relativeplantheight/cm
-0.600 0.785
-0.700 0.708
ƒKñ‚¤Üùë
Relativedriedmass/g
-0.600 0.746 0.680
-0.700 0.396 0.662
  ò(Note):犘<0.05;犘<0.01
\3 2¿犘犈犌¹$jk¾¶æ(„…3¿3p0UÀ;n(犉-)
Table3 VarianceanalysisofthreetraitsofsudangrassgermplasmunderdroughtstressusingtwoPEGconcentrations
no…†
Sourceofvariation
®ë¨
DF/e
ƒK”P5
Relativelivability/%
ƒKì
Relativeplantheight/cm
ƒKñ‚¤Üùë
Relativedrymass/g
GY
Varieties
21 20.21910672 26.36878391 18.7992174
ܨ
Concentration
1 25.49212002 99.77401013 13.45257931
GY×^X
Varieties×Concentration
21 0.764183061 1.374308399 1.851619437
  ò(Note):犘<0.05;犘<0.01
  P4G2ePEGܨš22e}ÅN‡EQB
þýg

}-0.6MPaš1‘Bþý«p(犇犜犐
0.6)GM{¬,}-0.7MPaš1‘Bþý«p
(犇犜犐0.7)GK{¬,ôL]‡EQBÛIC:
×

×2)。¿×2|}~,|}þýgBDo|
}¦22„‘*\‚zG›、š›、+、šã8ã5
y

!ŠyB犇犜犐0.6õ=犇犜犐0.7õzÆG1.7
~2.6=1.3~2.1,˒‘‚(1™’、‘89105’†
‘TS185’ƒ3e‡EQ,Ò+,‘‚(1™’B犇犜犐
0.6=犇犜犐0.7ÝJ—ì¦Ò~2e‡EQ;!Û
yB犇犜犐0.6õ=犇犜犐0.7õzÆG0~1.4=
-0.3~1.5,˒‘89103’!ƒ12e‡EQ;!Ù
yB犇犜犐0.6õ= 犇犜犐0.7õzÆG-1.1~
-0.5=-1.0~0.1,˒‘1098’,‘1540’=‘07
54’!3e‡EQ;!_yB犇犜犐0.6õ=犇犜犐0.7
õzÆG-3.4~-2.1=-2.7~1.2,˒‘SS2’,
‘0741’=‘1017’ƒ3e‡EQ;!ÈysøËÌ
‘06222’,Ò犇犜犐0.6õ=犇犜犐0.7õ–G-5.2。
õÕòVB5
,6eTáGY+ø‘SS2’ z•!_
y

þýgšã

Ò~5eGY–Ħ02y+,P
AHš›Bþýg

3 ~Yl
  Üa=€õá[17]vGìÕ5ŠYé@åýâ
çC›BÅ9

þýg—r즐Õ

$7=
O¦ŠY1QBéÂåýÅ9

ҟåýB¾DÞ
E5ҌÚyK

ìKz*¥i5BRg

ˆW•
¦Ò¼A@Ä8l˚ß

ÅGìÕO89


NqŒÐŠRg

T˜™B¥ÉŽUV

}
犘犈犌8000GKz`a9,림B:uEÅKL‰
KzÖLU{

Eˆ8*õUVƒÈ

âŸïñ;
WX}ÅN‡EQBKz*¥i5

•K89:u&#Bþýg;i¥ô“

Ñ
¥@íBPEGܨ×@CD。Åܨ‡Ú,` a
K‹pUV‘

]GY

B67W*

=jõ“
BDoWJ—

sVÓðµ¥ô‘Bþýâç

ÅPEGܨ‡*,‹pUV‘B{5ÃÚ,E
ÎUC

Ýù}JÕÍ^Bip½

vžqWâ
»µ¥ôÒþýg

£s

CmÑ¥œŠBPEG
ܨ;i^X

sWâ|/;UV‘Bþý
g

CmÑ¥BPEGܨ§¨‡‹,s.Åë(*
ˆù}Sá

0101
!5# “ ·!:}ÅN:u&#þýg¥ôNӆYùþýg§Œ
\4 ¶æ(„…ST0¸¹3
Table4 Droughttoleranceofsudangrassgermplasm
‡EQ
Genotype
-0.600MPa -0.700MPa
犚犔±SD/% 犚犎±SD/% 犚犇犕±SD/% 犚犔±SD/% 犚犎±SD/% 犚犇犕±SD/%
þýg
Droughttolerance
‚(1™ 98.8±1.8 50.0±1.6 46.7±1.5 91.3±1.8 40.5±0.1 39.7±0.3 ›
89105 94.4±7.9 57.0±3.1 41.7±0.1 86.1±11.8 50.1±1.9 39.8±0.0 ›
TS185 88.5±5.4 49.3±7.4 45.5±4.2 74.4±7.3 34.9±0.3 39.7±4.4 ›
89103 87.2±10.9 44.9±1.6 41.6±1.2 78.2±9.1 37.8±0.3 39.5±3.3 š›
1539 77.6±9.3 48.8±0.1 44.7±0.6 57.9±0.0 41.1±1.5 43.1±0.1 š›
86394 90.0±10.1 57.4±0.7 41.3±0.3 87.1±2.0 41.0±5.6 32.8±2.0 š›
8125 85.9±9.1 36.5±0.1 40.4±2.6 59.0±14.5 29.1±1.3 39.3±0.0 š›
8546 75.0±5.6 61.3±5.5 38.8±4.2 68.4±7.4 52.3±11.1 41.0±8.2 š›
Enroma 78.8±12.4 46.2±4.3 44.4±5.0 58.8±12.4 36.7±4.1 36.4±4.0 š›
SOR 82.9±1.9 49.3±1.1 38.8±0.8 77.6±5.6 42.8±0.7 34.3±2.1 š›
89104 80.6±13.6 47.2±4.3 37.2±4.4 84.7±5.9 39.0±0.6 32.2±1.7 š›
Z}2™ 83.8±5.3 52.3±2.4 37.3±0.1 73.8±8.8 37.0±1.3 33.5±0.4 š›
Brasero 65.3±9.8 50.3±2.0 39.5±0.3 55.6±0.0 42.2±9.1 40.0±4.3 š›
·RÚ2™ 78.6±10.1 52.0±0.7 40.3±1.3 70.0±6.1 35.0±2.8 30.2±3.6 š›
2680 85.9±5.4 58.7±4.0 31.9±0.5 79.5±3.6 47.5±3.4 30.6±3.8 š›
1098 64.7±8.3 41.7±1.8 36.1±2.6 42.6±12.7 40.8±4.2 37.2±4.2 +
1540 76.3±3.7 42.6±0.1 27.8±1.6 81.6±7.4 37.0±5.1 28.5±0.9 +
0754 55.9±10.0 38.9±2.4 38.8±0.7 36.8±6.2 32.3±4.7 35.2±3.8 +
SS2 53.8±1.8 37.0±6.9 28.3±3.2 47.5±0.0 32.8±0.5 31.1±2.0 šã
0741 36.5±1.9 33.1±3.8 28.6±0.7 16.2±7.6 25.0±2.2 35.2±1.4 šã
1017 36.8±2.1 24.2±7.2 25.0±4.7 27.9±12.7 23.5±2.8 26.8±6.7 šã
06222 18.4±3.7 13.8±1.2 17.9±2.6 15.8±0.0 12.5±3.0 14.6±2.1 ã
L–õ±¬»D
Mean±SD
72.5±20.7 45.1±11.3 36.9±7.4 62.3±22.7 36.9±8.9 34.6±6.3 -
noAp
/% 28.5 25.1 20.0 36.5 24.3 18.2 -
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