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作 者 ：李艳;鲁顺保;刘晓燕;江玉梅;李思光;朱笃;

期 刊 ：武汉植物学研究 2010年 28卷 1期 页码：38-42

关键词：华东黄杉；ISSR；遗传多样性；遗传结构；保护策略；

Keywords:Pseudotsuga gaussenii Flous, ISSR, Genetic diversity, Genetic structure, Conservation strategy,

摘 要 ：

采用ISSR分子标记技术分析了5个华东黄杉居群的遗传多样性水平和居群遗传结构。用10个引物对5个居群共76个样品进行扩增，共得到76条清晰的扩增带，其中69个位点为多态性位点。在物种水平上，多态性百分率 (PPB)为 90.79%，Nei’s基因多样性指数 (H)为0.3361，Shannon’s信息多样性指数 (I )为0.4974。在居群水平上，多态性位点百分率在56.58%～85.53%之间，Nei’s基因多样性指数为0.2084～0.2675，Shannon’s信息多样性指数为0.2695～0.4076。物种和居群遗传多样性水平都较高，居群间产生了一定的遗传分化(Gst=0.2366，Φst=26.88%)。由UPGMA聚类分析可知，在5个居群中，湖南阳明山两支与浙江临安先聚为一支，再与安徽休宁聚为一支，最后与江西三清山聚合。鉴于华东黄杉生境的特殊性和人为大量的砍伐，华东黄杉的数量逐渐减少，建议在华东黄杉分布区建立自然保护区，对其生存空间进行长期、有效的保护。

Abstract:Genetic diversity and genetic differentiation of Pseudotsuga gaussenii Flous were analyzed by using intersimple sequence repeat (ISSR) molecular technique.ISSR amplification was conducted with 10 primers for 76 individuals from 5 populations，and 69 polymorphic loci were detected from 76 loci，indicating high genetic variation at species level (PPB=90.79%，H=0.3361，I=0.4974) and at population level (PPB=56.58%-8.53%， H=0.2084-0.2681， I=02695-0.4076).A certain level of genetic differentiation occurred among populations (Gst=0.2366，Φst=26.88%).The program of genetic relationships among populations was constructed based on Nei’s gene distance.Two populations of Yangmingshan and Zhejiang clustered in a clade，then Anhui population and them clustered in a clade，and last Sanqingshan population clustered in a clade.In view of the genetic information available for .P.gaussenii. Flous，the number of .P.gaussenii. Flous has been dropping off.It is suggested that nature reserve should be founded in the distributing areas of P.gaussenii Flous to longtimely and effectively preserve the living space of P.gaussenii Flous.

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