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作 者 ：常珂玮, 沈君君, 李宗芸, 韩永华

期 刊 ：植物科学学报 2012年 30卷 4期 页码：402-406

关键词：西瓜；野生西瓜；比较基因组原位杂交；

Keywords:Citrullus lanatus, Wild Citrullus, Comparative genomic in situ hybridization,

摘 要 ：物种间亲缘关系的研究是杂交育种的理论基础,野生西瓜在西瓜育种中具有重要作用,然而目前对西瓜属物种间亲缘关系的研究十分有限,而且对西瓜属物种的分类问题还存在分歧。比较基因组原位杂交是分析物种间亲缘关系的有效手段,本研究以西瓜基因组DNA作探针, 分别对缺须西瓜、热迷西瓜、药西瓜和诺丹西瓜有丝分裂中期染色体进行了比较基因组原位杂交分析,揭示了西瓜属物种间的亲缘关系,同时对分类地位尚存在争议的诺丹西瓜的归属问题进行了分析,发现诺丹西瓜和甜瓜之间具有非常近的亲缘关系,本研究结果为西瓜与近缘种间的远缘杂交提供了重要的理论依据。

Abstract:Studies on the phylogenetic relationship among species are the theoretical basis of hybridization breeding.Wild Citrullus species play important roles in Citrullus lanatus breeding.However,research on relationships among C.lanatus and its close relatives are limited.Moreover,there are disagreements on the taxonomy of the genus Citrullus.Comparative genomic in situ hybridization is an effective method to analyze phylogenetic relationships.The mitotic metaphase chromosomes of Citrullus ecirrhosus,Citrullus rehmii, Citrullus colocynthis and Citrullus naudinianus were hybridized with labeled genome DNA from C.lanatus.The phylogenetic relationship was revealed among species and a close relationship between C.naudinianus and Cucumis melo was found by comparative genomic in situ hybridization with labeled genome DNA from C.melo.This research will provide a theoretical basis for hybridization breeding between watermelon and its close relatives.

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