利用我国长江流域大面积种植的高衣分、高产品种泗棉3号和西班牙陆地棉栽培品种Carmen，构建RIL作图群体，在3个环境中进行产量及其构成因素的QTLs标记和定位，研究了泗棉3号高产特性的分子机理。用2 523对SSR引物，进行双亲的多态性检测，其中62对（2.46%）有多态性，它们共产生65个稳定的多态性位点。通过复合区间作图，共检测到1个单株果节数、1个铃重、2个籽指、1个衣指和1个百粒籽棉重等8个产量构成因素的QTLs。单标记分析还在多环境中检测到17个产量构成因素的QTLs。与这些QTLs紧密连锁的分子标记可以用于对产量及其构成因素的分子标记辅助选择。
Molecular tagging of QTLs for yield and its components is a base of great significant to MAS for high yield breeding in cotton. Simian 3 have been one of the cotton cultivars most extensively grown in Yangtze River cotton-growing valley with high yield and high lint percentage, while Carmen is a Spanish cotton cultivar. QTL tagging of yield and its components in the RIL population from a cross between Simian 3×Carmen were conducted using 65 SSR markers having polymorphism between parents screened from a total of 2 523 pairs of SSR primers. The results showed that 1 QTL for fruit nodes per plant and 1 for boll size, 2 for seed index, 1 for lint index and 1 for seed-cotton weight of 100 seeds were detected in RIL population acress more than two environments based on composite interval mapping and marker multiple regression methods. Using the method of single-marker analysis, 17 QTLs controlling the yield and its components were screened in more than two environments. The molecular markers tightly linked with the QTLs controlling high yield and its components in this paper can be used for MAS in high-yield breeding program.
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