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作 者 ：韩锁义;张恒友;杨玛丽;赵团结;盖钧镒;喻德跃

期 刊 ：作物学报 2007年 33卷 12期 页码：2059-2062

关键词：大豆；60Coγ射线；EMS；突变体；

Keywords:Soybean, 60Coγrays, EMS, Mutant,

摘 要 ：应用⁶⁰Co γ射线和甲基磺酸乙酯(EMS)分别对“南农86-4”大豆种子进行诱变，并构建大豆突变体库。结果在M₃代分别获得40份和145份叶、茎、花、种子、子叶等性状变异的材料。在两种方式诱变的M₃代都获得蛋白质和油含量变化的材料，尤其在EMS诱变的M₃代中获得47份蛋白质含量比对照高5个百分点以上的材料，5份蛋白质和油总含量比对照高5个百分点以上的材料。这些突变体可以作为新的种质资源，构建的突变体库也有助于大豆功能基因组研究的开展。

Abstract:Soybean mutant population was constructed by treating the seeds of soybean “Nannong 86-4” with ⁶⁰Co γ rays and EMS respectively. In M₃ generations, 40 mutants of leaf, stalk, flower, seed and cotyledon were obtained by treatment of ⁶⁰Co γ rays, and 145 mutants by EMS. In addition, the mutants of protein and oil contents were obtained; there were 47 mutants with more than 5 percentages on protein content and 5 mutants with more than 5 percentages on total protein and oil content compared with control. These mutants will serve as new germplasm in breeding. The mutant population will be useful to study functional genomics in soybean.

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